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Thromb Haemost 2001; 85(05): 908-914
DOI: 10.1055/s-0037-1615767
Review Article
Schattauer GmbH

Increased ICAM-1 and PECAM-1 Transcription Levels in the Heart of Apo-E Deficient Mice in Comparison to Wild Type (C57BL6).

Kazem Zibara
1   Thrombosis Research Institute, London, UK
,
Kamel Chettab
2   INSERM U331/Faculté de Médecine RTH Laënnec, Lyon, France
,
Brigitte McGregor
2   INSERM U331/Faculté de Médecine RTH Laënnec, Lyon, France
,
Robin Poston
3   Department of Experimental Pathology, UMDS, Guy‘s Hospital, London, UK
,
John McGregor
2   INSERM U331/Faculté de Médecine RTH Laënnec, Lyon, France
› Author Affiliations
Further Information

Publication History

Received 26 June 2001

Accepted after resubmission 14 December 2000

Publication Date:
11 December 2017 (online)

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Summary

Adhesion molecules and chemoattractants are thought to play a critical role in the homing of leukocytes to sites of vascular lesions. Apo-E deficiency in mice creates an atherosclerotic model that mimics vascular lesions in man. Little is known on the effect of Apo-E deficiency on expression of adhesion molecules in the hearts of these animals. In this study, male C57BL6 and Apo-E deficient mice were fed a chow diet over periods of time (0 to 20 weeks). The transcription levels of major adhesion molecules (ICAM-1, PECAM-1), present in the heart, were followed by northern blots. Immunohistochemistry was used to localize these adhesion molecules in the heart. Results show a significant increase in gene transcription levels of ICAM-1 and PECAM-1 in Apo-E animals, but not wild type, at 16 and 20 weeks of chow diet. Such increase in levels of transcription was not observed in younger Apo-E and C57BL6 animals (0, 6 weeks of diet). ICAM-1 and PECAM-1 were strongly expressed in the endocardium and heart microvessels. In contrast, VCAM-1 was poorly stained, with only an occasional expression on the endocardium and arterioles. Enhanced gene expression levels of heart ICAM-1 and PECAM-1 observed in Apo-E deficient mice, but not in control animals, appears to induce the initial stages of an inflammatory reaction. Such observations, not previously reported, may induce heart vascular remodeling.

Keywords

Atherosclerosis - adhesion molecules - apolipoprotein E - knockout mice - heart - northern
 

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