Human Endothelial Cells Maintain Anti-Aggregatory Activity for Platelets during Apoptosis

Wei Xu; Emmanuel J. Favaloro; Heather Medbury; Hans Zoellner

doi:10.1055/s-0037-1615768

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2001; 85(05): 915-923
DOI: 10.1055/s-0037-1615768

Review Article

Schattauer GmbH

Human Endothelial Cells Maintain Anti-Aggregatory Activity for Platelets during Apoptosis

Authors

Wei Xu

¹Department of Oral Pathology and Oral Medicine, University of Sydney, Westmead Hospital Dental Clinical School, Westmead Hospital, NSW, Australia
Emmanuel J. Favaloro

²Department of Haematology, Westmead Hospital, Australia
Heather Medbury

³Department of Surgery, Westmead Hospital, Australia
Hans Zoellner

¹Department of Oral Pathology and Oral Medicine, University of Sydney, Westmead Hospital Dental Clinical School, Westmead Hospital, NSW, Australia

Abstract

Summary

Despite evidence of elevated levels of tissue factor and platelet binding by apoptotic endothelial cells, microthrombi do not appear to be associated with apoptotic endothelium and this suggests maintained anti-aggregatory activity for platelets. We report that anti-aggregatory activity is maintained by apoptotic endothelium obtained by serum and or matrix deprivation, which we propose as models for apoptotic endothelial cells released during microvascular remodelling and traumatic detachment respectively. Both apoptotic and non-apoptotic endothelium had strong anti-aggregatory activity for platelets stimulated with either ADP or thrombin. Inhibition experiments using L-NAME and indo-methacin indicated a role for nitric oxide and prostacyclin in this activity. Experiments with latex beads further confirmed that inhibited platelet aggregation by endothelium was not merely a non-specific phenomenon. These data support the idea that EC maintain active anti-thrombotic activity during apoptosis, consistent with maintained urokinase levels and canalicular fragmentation reported elsewhere.

Keywords

Endothelium - apoptosis - platelets - aggregation

Full Text

References

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