The 33-kDa Platelet α-granule Membrane Protein (GMP-33) Is an N-terminal Proteolytic Fragment of Thrombospondin

Conchi Damas; Tom Vink; Karel H. Nieuwenhuis; Jan J. Sixma

doi:10.1055/s-0037-1616152

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2001; 86(03): 887-893
DOI: 10.1055/s-0037-1616152

Review Articles

Schattauer GmbH

The 33-kDa Platelet α-granule Membrane Protein (GMP-33) Is an N-terminal Proteolytic Fragment of Thrombospondin

Conchi Damas

¹Department of Haematology and Graduate School for Biomembranes, University of Utrecht, The Netherlands

,

Tom Vink

¹Department of Haematology and Graduate School for Biomembranes, University of Utrecht, The Netherlands

,

Karel H. Nieuwenhuis

¹Department of Haematology and Graduate School for Biomembranes, University of Utrecht, The Netherlands

,

Jan J. Sixma

¹Department of Haematology and Graduate School for Biomembranes, University of Utrecht, The Netherlands

› Author Affiliations

Abstract

Summary

GMP-33 is a platelet membrane associated protein that is recognised by RUU-SP 1.77, an antibody raised against activated platelets. GMP-33 is predominantly associated with the membrane of platelet α-granules and it is translocated to the plasma membrane upon platelet activation (Metzelaar et al. Blood 1992; 79: 372-9). In this study we have isolated the protein by immunoaffinity chromatography. The N-terminus was sequenced and was identical to the N-terminal sequence of human thrombospondin. The protein was N-glycosylated and bound to heparin as would be expected of the N-terminal part of thrombospondin. RUU-SP 1.77 reacted only with reduced thrombospondin. Plasmin and trypsin digestion of thrombospondin yielded fragments of approximately the same size as GMP 33 that reacted with RUU-SP 1.77 after reduction. No evidence for alternative splicing was found. We postulate that GMP 33 is an N-terminal proteolytic fragment of thrombospondin that is membrane associated.

Keywords

Thrombospondin - platelet α-granule - platelet activation - platelet membrane

Full Text

References

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