Hepatic Thrombopoietin mRNA Is Increased in Acute Inflammation

Eva-Maria Wolber; Joachim Fandrey; Urszula Frackowski; Wolfgang Jelkmann

doi:10.1055/s-0037-1616745

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2001; 86(06): 1421-1424
DOI: 10.1055/s-0037-1616745

Review Article

Schattauer GmbH

Hepatic Thrombopoietin mRNA Is Increased in Acute Inflammation

Eva-Maria Wolber

¹Institute of Physiology, Medical University of Luebeck, Luebeck, Germany

,

Joachim Fandrey

²Institute of Physiology, University of Essen, Essen, Germany

,

Urszula Frackowski

¹Institute of Physiology, Medical University of Luebeck, Luebeck, Germany

,

Wolfgang Jelkmann

¹Institute of Physiology, Medical University of Luebeck, Luebeck, Germany

› Institutsangaben

Abstract

Summary

The plasma concentration of thrombopoietin (TPO) in general is inversely related to the mass of platelets and megakaryocytes. However, reactive thrombocytosis of inflammatory disease is accompanied by elevated TPO levels. To investigate whether the rate of TPO mRNA expression is altered during acute inflammation, rats were injected with bacterial lipopolysaccharide (LPS). After 6 h, total RNA from liver and kidney was reverse transcribed and analyzed by competitive PCR for TPO and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). LPS-treated rats showed a significant increase in hepatic TPO mRNA concentration. The ratio of TPO to GAPDH mRNA was 3.5 ± 0.6% in the livers of control rats and 8.3 ± 2.0% in the livers of LPS-treated rats (mean ± SD). Thus, reactive thrombocytosis of inflammatory disease might result from an increase in hepatic TPO production. Since platelets are involved in the immune reaction, reactive thrombocytosis may be a mechanism of host defense.

Keywords

Thrombopoietin - inflammation - reactive thrombocytosis - acute phase reaction - LPS

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