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Nuklearmedizin 1978; 17(02): 66-69
DOI: 10.1055/s-0037-1620672
Originalarbeiten – Original Articles
Schattauer GmbH

Production of Lyophilized Albumin Macroaggregates and Labelling with 99mTc

Die Herstellung von lyophilisierten Albumin Makromolekülen und Markierung mit 99mTc
V. Jovanović
1   From the Boris Kidrič Institute of Nuclear Sciences, Vinča-Belgrade, Yugoslavia
,
J. Čvorić
1   From the Boris Kidrič Institute of Nuclear Sciences, Vinča-Belgrade, Yugoslavia
,
O. Jovanović
1   From the Boris Kidrič Institute of Nuclear Sciences, Vinča-Belgrade, Yugoslavia
,
Č. Teofilovski
1   From the Boris Kidrič Institute of Nuclear Sciences, Vinča-Belgrade, Yugoslavia
› Author Affiliations
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Publication History

Received: 09 January 1978

Publication Date:
07 January 2018 (online)

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Optimal conditions of HSA aggregation in acetate buffer (pH = 4.6) were investigated. With the aim of obtaining a higher percentage of fixation in the lungs, the aggregates were rinsed three times with acetate buffer. A MAA suspension was lyophilized with SnCl22H2O (10 μg/ml) solution. The lyophilized MAA was then labelled by adding pertechnetate of the desired activity in a volume of up to 5 ml. The radiochemical purity of 99mTc-MAA, greater than 95%, was determined by the methods of thin layer chromatography and low-voltage electrophoresis. All MAAs were uniform in shape whereas the size varied between 20 and 100 μm.

Biodistribution studies have shown the maximum radioactivity to be deposited in the animals' lungs immediately after application of 99mTc-MAA.

Es wurden die zur Aggregation von HSA in Azetatpuffer (pH 4.6) nötigen Optimalbedingungen untersucht. Zwecks Erreichung eines höheren Fixierungsgrades in der Lunge wurden die Aggregate dreimal mit Azetatpuffer gewaschen. Die MAA-Suspension wurde mit einer Lösung von SnCl22H2O (10 μg/ml) lyophilisiert.

Das Markierungsverfahren für lyophilisierte MAAs besteht in der Zugabe von Pertechnetat in der gewünschten Aktivität in einem Volumen bis zu 5 ml.

Die radiochemische Reinheit vom 99mTc-MAA ist höher als 95 % und wurde mittels Dünnschicht-Chromatographie und Niederspannungs-Elektrophorese bestimmt. Die MAAs waren uniform in ihrer Form während die Größe zwischen 20–100 μm schwankte.

Durch Untersuchungen der Bioverteilung wurde festgestellt, daß die maximale Aktivität in den Lungen der Versuchstiere unmittelbar nach der Applikation von 99mTc-MAA deponiert wird.

 

  • References

  • 1 Spencer R, Rad M, Seaton A, Little W. Lung scanning in pulmonary embolism. Brit. J. Radiol 1968; 41: 885.
    CrossrefPubMedGoogle Scholar
  • 2 Bruce Sodee D. Advances in medical scanning. Nucleonics 1964; 22: 47.
    Google Scholar
  • 3 Taplin G. V. Preparation of colloidal suspensions of human serum albumin 131I for estimating liver blood flow and reticuloendothelial system functions in man. Report UCLA 481-1961.
    Google Scholar
  • 4 Jovanovic V. Chemical and biological properties of 131I-albu-min macroaggregates. Nucl.-Med. (Stuttg.) 1974; XII: 328.
    PubMedGoogle Scholar
  • 5 Charamza O. Preparation and control of 99mTc-albumin macroaggregates for scanning of lungs. Ceskoslov. farm 1973; 22: 380.
    Google Scholar
  • 6 Gwyther M, Field O. Aggregated 99mTc-labelled albumin for scintiscanning. Int. J. appl. Radiat 1966; 17: 485.
    CrossrefPubMedGoogle Scholar
  • 7 Cragin D, Webber M, Victery K. et al. Technique for the rapid reparation of lung scan particles using 99mTc-sulfur and human serum. J. nucl. Med 1969; 10: 621.
    PubMedGoogle Scholar
  • 8 Rhodes A. Lung scanning with 99Tc-microspheres. Radiology 1971; 99: 613.
    CrossrefPubMedGoogle Scholar
  • 9 Davis M. A. 99mTc-iron hydroxide aggregates. Evaluation of a new lung scanning agent. Radiology 1970; 85: 347.
    Google Scholar
  • 10 Licińska I, Lucka B, Pszona B, Sadowska B, Wagner M. Biological and chemical investigations of 13II-albu-min macroaggregates used for lung scintigraphy. Nucl.-Med. (Stuttg.) 1971; XI: 51.
    Google Scholar
  • 11 Zolle I, Oniciu L, Höfer R. Contribution to the study of the mechanism of labelling human serum albumin (HSA) with technetium-99m. Int. J. appl. Radiat 1973; 24: 621.
    CrossrefPubMedGoogle Scholar
  • 12 Zolle I, Rhodes B, Wagner H. N. Preparation of metabolizable radioactive human microspheres for studies of the circulation. Int. J. appl. Radiat 1970; 21: 155.
    CrossrefPubMedGoogle Scholar