The Extraction from the Blood of Phosphate Compounds by Canine Bone

S. P. F Hughes; H. W. Wahner; P. J. Kelly; D. R. Davies

doi:10.1055/s-0037-1620890

Nuklearmedizin - NuclearMedicine, Inhaltsverzeichnis

Nuklearmedizin 1979; 18(03): 137-141
DOI: 10.1055/s-0037-1620890

Originalarbeiten – Original Articles

Schattauer GmbH

The Extraction from the Blood of Phosphate Compounds by Canine Bone

Die Extraktion von Phosphatverbindungen aus dem Blut durch das Knochengewebe von Hunden

S. P. F Hughes

¹ From the Mayo Clinic and Mayo Foundation, Rochester, Min. U.S.A.

,

H. W. Wahner

¹ From the Mayo Clinic and Mayo Foundation, Rochester, Min. U.S.A.

,

P. J. Kelly

¹ From the Mayo Clinic and Mayo Foundation, Rochester, Min. U.S.A.

,

D. R. Davies

¹ From the Mayo Clinic and Mayo Foundation, Rochester, Min. U.S.A.

› Institutsangaben

Abstract

Summary

Uptake by bone of ^99mTc-labeled pyrophosphate (PP^j) and ethane-1-hydroxy-1,1-diphosphonate (EHDP) involves passage through capillaries in the Haversian system, extracellular fluid space, and membrane of osteoblasts before adsorption onto available apatite surfaces. Passage through capillaries was studied by the outflow-dilution technique in a canine tibia model in mongrel dogs. slCr-labeled albumin (nondiffusible reference tracer), sucrose (diffusible reference tracer), PPⁱ, and EHDP were injected into the isolated nutrient tibial artery. Blood was collected from the ipsilateral femoral vein every 15 sec for 2 min. E^max, the apparent extraction at the time of the peaks of the dilution curves, is influenced little by back diffusion; for PP, E^max (mean ± SD) = 0.42 ± 0.08 (N = 4); for EHDP, E^max = 0.27 ± 0.05 (N - 10). Net extraction (apparent fractional retention by (pone at 2 min) was 0.36 ± 0.1 for PP. E^max for 8sSr chloride and 18F sodium in our laboratory is 0.69 ± 0.11 (N = 14) and 0.70 ± 0.008 (N = 9), respectively. Permeabilities (P) were calculated by PS = -Fs loge (1-E^max), where Fs = plasma flow and S = capillary surface; the ratio of P for EHDP to P for sucrose was 0.71 which is similar to the ratio of diffusion coefficients, 0.78. The data suggest that EHDP and probably also PP^j pass through the capillaries by passive diffusion. As expected, the extraction through capillary walls for EHDP and PP^j was lower than that for 8SSr and 18F because of their larger molecular size.

Zusammenfassung

Die Akkumulation von ^99mTc-markiertem Pyrophosphat (PP^j) und Diphosphonat (EHDP) ist abhängig von der Passage des Radiopharmakons durch die Kapillaren des Haversschen Systems im Knochen, die Passage durch den extrazellulären Raum und durch die Osteoblastenmembrane, bevor es an der Apatitoberfläche absorbiert werden kann. In diesem Report wird über die Passage des ^99mTc-markierten Radiopharmakons durch die Kapillaren des Haversschen Systems berichtet. Das Problem wurde mit Hilfe der Ausfluß-Verdünnungs-Technik (outflow-dilution technique) und einem schon früher beschriebenen isolierten Tibiapräparat vom Hund studiert. ⁵¹Cr-markiertes Albumin diente als nicht-diffundierende und Saccharose als diffundierende Referenzmarkierung. PP^j und EHDP wurden in die isolierte Tibiaarterie injiziert und der venöse Ausfluß gesammelt. Es wurden sowohl die Extraktionsraten als auch die Permeabilität bestimmt. Früher veröffentlichte Formeln, die für dieses experimentelle Modell entwickelt wurden, fanden Verwendung. Die Resultate zeigen, daß EHDP und wahrscheinlich auch PP^j durch die Kapillaren via passive Diffusion gelangen. Die Passage durch die Kapillaren war langsamer für EHDP und PP^j, verglichen mit 87mSr oder 18F, bedingt durch die größeren Moleküle der "Tc-markier- ten Radiopharmaka.

Volltext

Referenzen

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