Conversion of ³H-Testosterone to Dihydrotestosterone in Human Hypertrophic Prostatic Tissue

Summary

The aim of the study was to develop a simple method for the determination of the conversion of testosterone to 5α-dihydrotestosterone (5α-DHT) after incubation of human hypertrophic prostatic tissue with ³H-testosterone. The mean conversion rate of ^3H-testosterone to 5α-DHT in hypertrophic prostatic tissue was found to be higher than in normal and carcinomatous tissue. The results indicate that androgen metabolism in the hypertrophic prostatic gland is enhanced.

Zusammenfassung

Das Ziel der Untersuchung war die Entwicklung einer einfachen Methode der Bestimmung der Umwandlung von Testosteron in 5α-Dihydrotestosteron (5α-DHT) nach der Inkubation menschlichen hypertrophischen Prostatagewebes mit ³H-Testosteron. Der durchschnittliche Konversionsindex des ^3H-Testosterons in 5α-DHT in hypertrophischem Prostatagewebe war höher als in normalem oder karzinomatösem Gewebe. Die Ergebnisse weisen darauf hin, daß der Androgenstoffwechsel in der hypertrophischen Prostatadrüse erhöht ist.

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