Radioautographic Observations on Transfer of Thymidine-3H in the Trophoblastic Giant Cells in the Early Pregnancy of Rats

G. Blumen; L. Kulay Jr.; N. Marques de Castro; D. Delascio

doi:10.1055/s-0037-1621372

Nuklearmedizin - NuclearMedicine, Table of Contents

Nuklearmedizin 1968; 07(02): 195-202
DOI: 10.1055/s-0037-1621372

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

Radioautographic Observations on Transfer of Thymidine-³H in the Trophoblastic Giant Cells in the Early Pregnancy of Rats

Observations autoradiographiques sur le transfer de la ³H-thymidine dans les cellules géantes trophoblastiques des rats au début de la graviditéRadioautographische Betrachtungen über den Transfer von ³H-Thymidin in die Trophoblasten-Riesenzellen während der frühen Schwangerschaft der Ratte

G. Blumen

¹Departments of Morphology and Obstetrics, Escola Paulista de Medicina, São Paulo, Brasil

,

L. Kulay Jr.

¹Departments of Morphology and Obstetrics, Escola Paulista de Medicina, São Paulo, Brasil

,

N. Marques de Castro

¹Departments of Morphology and Obstetrics, Escola Paulista de Medicina, São Paulo, Brasil

,

D. Delascio

¹Departments of Morphology and Obstetrics, Escola Paulista de Medicina, São Paulo, Brasil

› Author Affiliations

Abstract

Summary

The authors tried to relate the dose of injected ³H-thymidine with the incorporation by secondary trophoblastic giant cells from the 8th to the 11th day of the rat’s pregnancy. It appeared that the labelling of these cells during this period depends on the amount of the labelled compound administered.

Employant ³H-thymidine du 8éme au 11éme jour d’enceinte en rates, les auteurs ont cherché la relation des quantités injectées avec l’incorporation par les cellules géantes trophoblastiques secondaires. Il semble qui la marcation de cettes cellules dans de période la est en dépendance de la quantité d’isotope injecté.

Verfasser versuchten, durch Gaben von ³H-Thymidin vom 8. bis zum 11. Schwangerschaftstage bei der Ratte die injizierten Mengen mit der Einverleibung durch die sekundären Trophoblastriesenzellen in Zusammenhang zu bringen. Allem Anschein nach hängt die Markierung solcher Zellen in dieser Zeitspanne von der Menge der zugeführten markierten Verbindung ab.

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References

References
1 Alden R.H.. Implantation of the rat egg. III. Origin and development of primary trophoblast giant cells. Amer. J. Anat. 83: 143 1948;
2 Brasseur L., Issac-Mathy M., DeMeyer R.. Comparative study of the glycogen content of fetal liver and the placenta in pregnant rats. Ann. Endocr. (Paris) 19: 431 1958;
3 Bridgman J.. A morphological study of the development of the placenta of the rat. J. Morph. 83: 61 1948;
4 Bulmer D., Dickson A.D.. Observations on carbohydrate material in the rat placenta. J. Anat. 94: 46 1960;
5 Deane H.W., Rubin B.L., Driks E.C., Lobel B.L., Leipsner G.. Trophoblastic giant cells in placenta of rats and mice and their probable role in steroid-hormon production. Endocrinology 70: 407 1962;
6 Dickson A.D., Bulmer D.. Observation on the placental giant cells of the rat. J. Anat. 94: 419 1960;
7 Doniach I., Pelc S.R.. Autoradiograph technique. Brit. J. Radiol. 23: 184 1950;
8 Duval M.. Le placenta des rongeurs. III. Le placenta de la souris et du rat. J. Anat. 27 24, 344 515 1891;
9 Hagerman D.P., Villee C.A.. Transport functions of the placenta. Physiol. Rev. 40: 313 1960;
10 Jackson H.. Antifertility substances. Pharmacol. Rev. 11: 135 1959;
11 Jollie W.P.. Radioautographic observations on variations in desoxyribonucleic acid synthesis in rat placenta with increasing gestational age. Amer. J. Anat. 114: 161 1964;
12 Koransky W., Ullberg S.. Autoradiographic investigations of ¹⁴C-labelled thalidomide and glutethimide in pregnant mice. Proc. Soc. exp. Biol. Med. 116: 512 1964;
13 Koremblit E., F.A. Uranga Imaz (h) del M., Ciarniello C.. Influencias de la nutricion sobre el estado gravido puerperal y el reciénnascido. Obst, y Ginec. Lat-Amer. 5 /6: 218 1967;
14 Koren Z., Shafrir E.. Placental transfer of the free fatty acids in the pregnant rats. Proc. Soc. exp. Biol. Med. 116: 411 1964;
15 Koren Z., Pfeifer Y., Sulman F.G.. Distribution and placental transfer of C¹⁴-serotonin in pregnant rats. Amer. J. Obstet. Gynec. 95: 290 1966;
16 Kulay Jr L. Contribuição para o estudo histoquímico de polissacarídeos, lipídeos e proteínas da placenta de rata (Rattus norvegicus albinus), durante o período functional. Matern. e Inf. 25: 493 1966;
17 Leathern J.H.. Hormonal and nutritonal influences on male reprodutive system. Anat. Rec. 118: 323 1954;
18 Morrison S.D.. The nitrogen balance of pregnant rats. J. Physiol. 133: 167 1956;
19 Padykula H.A., Richardson D.. A correlated histochemical and biochemical study of glycogen storage in the rat placenta. Am. J. Anat. 112: 215 1963;
20 Quigley H., Phillips L.L., McKay D.G.. Transport of radioactive sodium across the rat placenta in experimental toxemia of pregnancy. Amer. J. Obstet. Gynec. 91: 377 1965;
21 Shipley R.A., Chudzick E.B., Curtiss C., Price J.W.. Effect of lipotrophic deficient diets and steroid hormones on the pregnant rat. Metabolism 2: 165 1953;
22 Taylor J.H., Woods P.S., Hughes W.L.. The organization and duplications of chromosomes as revelad by autoradiographic study using tritium labelled thymidine. Proc. Nat. Acad. Sci. 43: 122 1957;
23 Tremblay P.C., Sybulski G., Maughan B.. Role of the placenta in fetal malnutrition. Amer. J. Obstet. Gynec. 91: 597 1965;
24 Wegener K., Hollweg S., Maurer W.. Autoradiographische Bestimmung der DNS-Verdopplungszeit und anderer Teil-Phasen des Zell-Zyklus bei fetalen Zellarten der Ratte. Z. Zellforsch. 63: 309 1964;
25 Widdas W.F.. Transport mechanisms in the foetus. Brit. med. Bull. 17: 107 1961;
26 Wislocki G.B., Deane H.W., Dempsey E.W.. The histochemistry of the rodent’s placenta. Amer. J. Anat. 78: 281 1946;
27 Zunker H.O., Phillips L.L., McKay D.G.. Potassium transport in kidney and placenta in normal pregnancy and in experimental toxemia. Amer. J. Obstet. Gynec. 91: 369 1965;