Perikardiales malignes Mesotheliom beim Beagle

Zusammenfassung

Gegenstand und Ziel: Bei einer neunjährigen Beaglehündin, die nach kurzer Krankheitsdauer (35 Tage) wegen eines rezidivierenden serohämorrhagischen Thoraxergusses euthanasiert werden musste, fand sich bei der Sektion eine Tumorbildung am parietalen Perikard. Ziel der Arbeit war, die Neoplasie ihrem Herkunftsgewebe zuzuordnen.

Material und Methode: Das perikardiale Tumorgewebe wurde nach verschiedenen Methoden gefärbt und histologisch untersucht. Zudem erfolgten immunhistochemische Untersuchungen mit Markern wie Zytokeratin, Vimentin, Thrombomodulin und Calretinin sowie eine elektronenmikroskopische Untersuchung.

Ergebnisse: Das in multiplen Plaques am Perikard lokalisierte Tumorgewebe war histologisch durch vom Deckepithel ausgehende, zelldichte Proliferationen epitheloider, mitosenreicher Tumorzellen in tubulopapillärer Wuchsform charakterisiert. Die Tumorzellen zeigten starke zytoplasmatische Positivität für den Pan-ZytokeratinAK Klon AE1/3, reagierten jedoch sowohl auf Zytokeratin 5 und 6 als auch auf Calretinin-AK negativ. Dagegen wiesen sie eine starke zytoplasmatische Positivität für den AK Thrombomodulin auf. Mehrheitlich waren die Tumorzellen vimentinnegativ, nur das Zytoplasma einzelner Zellen in isolierten Tumorzellfoci reagierte positiv auf Vimentin. Starke zytoplasmatische Positivität ließ sich jedoch für Bindegewebszellen und elastische Gefäßmembranen mit dem Vimentin-AK darstellen. Elektronenmikroskopisch fielen zahlreiche dicht stehende, lange und dünne, von der freien Zelloberfläche ausgehende und in interzelluläre Lumina hineinragende Mikrovilli auf.

Schlussfolgerung: Die Befunde sprechen dafür, dass es sich bei den Tumorzellen um neoplastisches Mesothel handelt, der perikardiale Tumor also ein malignes Mesotheliom darstellt.

Summary

Objective: A nine-year-old female beagle dog was euthanized aftera short period of illness (35 days) due to recurrent sero-hemorrhagic effusions in the thorax. Upon pathological examination, a tumor in the parietal pericardium was observed. The aim of the study was to determine the tissue origin of the tumor.

Material and methods: The pericardial tumor tissue was stained by various methods and histologically examined. In addition, immunohistochemical examinations with the markers cytokeratin, vimentin, thrombomodulin and calretinin, and electronmicroscopic examinations were carried out.

Results: The tumor tissue occurred as multiple plaques on the pericardium and was histologically characterized by cell-dense tubulo-papillary extrusions of mitotic-rich epitheloid cells arising from the mesothelium. The tumor cells showed strong positive reaction to the pancytokeratin antibody (clone AE1/3) and thrombomodulin, and were negative to the antibodies cytokeratin 5 and 6 as well as calretinin. Most tumor cells did not show a reaction against the vimentin antibody, with the exception of a positive reaction in the cytoplasm ofa few cells in isolated tumor foci. The strong positive cytoplasmatic reaction against vimentin antibody is indicative of connetive tissue or the elastic membrane of blood vessels. Electronmicroscopic examinations showed numerous, densly occurring, long and thin microvilli originating at the open cell surface of the tumor cells which extended into the intercellular lumina.

Conclusions: Histology, immunohistology and electronmicroscopic examinations confirm the tumor cells to be neoplastic mesothelium and we conclude the tumor to be a malignant mesothelioma.

Schlüsselwörter

Beagle - Perikard - malignes Mesotheliom - Pathologie - Immunhistochemie - Elektronenmikroskopie

Key words

Beagle dog - Pericard - Malignant mesothelioma - Pathology - Immunohistochemistry - Electron microscopy

Eingegangen: 23.12.2004; akzeptiert: 08.03.2005

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