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Tierarztl Prax Ausg K Kleintiere Heimtiere 2006; 34(03): 201-207
DOI: 10.1055/s-0037-1622534
Andere Tierarten
Schattauer GmbH

Das Immunsystem der marinen Säugetiere

Teil 2: Morphologie, Immunphänotypisierung und Pathologie der lymphatischen OrganeThe immune system of marine mammalsPart 2: Morphology, immunophenotyping and pathology of lymphoid organs
A. Beineke
1   Aus dem Institut für Pathologie (Direktor: Prof. Dr. W. Baumgärtner, Ph. D.) der Stiftung Tierärztliche Hochschule Hannover, und dem
,
U. Siebert
2   Forschungs- und Technologiezentrum Westküste, Hafentoern, Büsum
,
W. Baumgärtner
1   Aus dem Institut für Pathologie (Direktor: Prof. Dr. W. Baumgärtner, Ph. D.) der Stiftung Tierärztliche Hochschule Hannover, und dem
› Institutsangaben
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Publikationsverlauf

Eingegangen: 20. Juli 2005

akzeptiert: 28. Juli 2005

Publikationsdatum:
05. Januar 2018 (online)

    Lizenzen und Reprints

Zusammenfassung

Die Morphologie der primären und sekundären lymphatischen Organe von Walen und Delphinen zeigt weitreichende Homologien zu denen terrestrischer Säugetiere. Es finden sich jedoch artspezifische anatomische Besonderheiten, wie beispielsweise die komplexen lymphoepithelialen Drüsen im Kehlkopf der Cetaceen (Waltiere), die eine Anpassung an den marinen Lebensraum darstellen. Weiterhin müssen physiologische Veränderungen, wie die altersabhängige Thymusatrophie und Zystenbildungen in der “analen Tonsille” der Waltiere, bei der Interpretation pathologischer Befunde dieser lymphatischen Einrichtungen mit berücksichtigt werden. Der Einsatz von spezifischen Leukozytenmarkern ermöglicht die nähere Charakterisierung von immunologischen und entzündlichen Reaktionen auf zellulärer Ebene. Mittels Durchflusszytometrie und Immunhistologie können so verschiedene T-und B-Zell-Subpopulationen sowie antigenpräsentierende Zellen der Wale und Delphine phänotypisiert werden. Für diese Tierarten liegen jedoch bisher nur wenige Berichte über kreuzreagierende oder speziesspezifische Antikörper vor. Pathologische Veränderungen in lymphatischen Organen finden sich infolge von verschiedenen neoplastischen und infektiösen Erkrankungen. In diesem Zusammenhang kommt es im Verlauf der verheerenden Morbillivirus-Seuchenzüge unter anderem zur generalisierten lymphatischen Depletion der infizierten Meeressäuger. Weiterhin führen chronische Erkrankungen und Abmagerung zum Verlust von Immunzellen und damit zu einer verminderten Resistenz gegenüber opportunistischen Infektionen. Neuere Studien geben vermehrt Hinweise auf die immuntoxische Wirkung verschiedener Schadstoffe in der Umwelt und die daraus resultierende Immunsuppression bei Walen, Delphinen und Robben.

Summary

The majority of primary and secondary lymphoid organs of whales and dolphins are homologous to those organs of terrestrial mammals. However, some unique anatomical structures, such as the complex lymp-hoepithelial laryngeal glands in cetaceans represent an adaptation to the marine environment. Additionally, physiologic changes, such as age-related thymic atrophy and cystic degeneration of the “anal tonsil” of whales have to be considered in the interpretation of pathologic lesions in these lymphoid structures. Specific leukocyte-markers enable the detailed characterization of cellular responses during immunological and inflammatory processes. Using immunological methods, such as flow cytometry and immunohistochemistry, different T and B cell subsets as well as antigen-presenting cells can be detected in whales and dolphins. However, onlya few reports mention the usefulness of cross-reacting or species-specific antibodies in these animals. Pathological lesions in lymphoid organs can be observed during several infectious and neoplastic diseases in marine mammals. Regarding this, systemic morbillivirus infections lead to devastating mass mortalities associated with severe lymphoid depletions in cetaceans and pinnipeds worldwide. Furthermore, chronic diseases and starvation are associated with a loss of functional lymphoid cells and decreased resistance against opportunistic infections. Additionally, there is growing evidence for the immunotoxic effect of different environmental contaminants and their immunosuppressive potentials in whales, dolphins and seals.

Schlüsselwörter

Meeressäugetiere - lymphatische Organe - Immunphänotypisierung - lymphatische Depletion

Key words

Marine mammals - lymphoid organs - immunophenotype - lymphoid depletion
 

  • Literatur

  • 1 Beineke A, Siebert U, McLachlan M, Bruhn R, Thron K, Failing K, Müller G, Baumgärtner W. Investigations of the potential influence of environmental contaminants on the thymus and spleen of harbor porpoises (Phocoena phocoena). Environ Sci Technol 2005; 39: 3933-8.
    CrossrefPubMedGoogle Scholar
  • 2 Beineke A, Siebert U, Wünschmann A, Stott JL, Prengel I, Kremmer E, Baumgärtner W. Immunohistochemical investigation of the cross-reactivity of selected cell markers from various species for characterization of lymphatic tissues in the harbour porpoise (Phocoena phocoena). J Comp Pathol 2001; 05: 311-7.
    PubMedGoogle Scholar
  • 3 Bernier J, De Guise S, Martineau D, Beland P, Beaudet M, Fournier M. Purification of functional T lymphocytes from splenocytes of the beluga whales (Delphinapterus leucas). Dev Comp Immunol 2000; 24: 653-62.
    CrossrefPubMedGoogle Scholar
  • 4 Clark LS, Turner JP, Cowan DF. Involution of lymphoid organs in bottlenose dolphins (Tursiops truncatus) from the western Gulf of Mexico: implications for life in an aquatic environment. Anat Rec A Discov Mol Cell Evol Biol 2005; 282: 67-73.
    PubMedGoogle Scholar
  • 5 Cowan DF. Involution and cystic transformation of the thymus in the bottlenose dolphin, Tursiops truncatus. Vet Pathol 1994; 31: 648-53.
    CrossrefPubMedGoogle Scholar
  • 6 Cowan DF, Smith TL. Morphology of complex lymphoepithelial organs of the anal canal (“anal tonsil”) in the bottlenose dolphin, Tursiops truncatus. J Morphol 1995; 223: 263-8.
    CrossrefPubMedGoogle Scholar
  • 7 Cowan DF, Smith TL. Morphology of the lymphoid organs of the bottlenose dolphin, Tursiops truncatus. J Anat 1999; 194: 505-17.
    CrossrefPubMedGoogle Scholar
  • 8 De Guise S, Bernier J, Martineau D, Beland P, Fournier M. Phenotyping of beluga whale blood lymphocytes using monoclonal antibodies. Dev Comp Immunol 1997; 21: 425-33.
    CrossrefPubMedGoogle Scholar
  • 9 De Guise S, Erickson K, Blanchard M, DiMolfetto L, Lepper HD, Wang J, Stott JL, Ferrick DA. Monoclonal antibodies to lymphocyte surface antigens for cetacean homologues to CD2, CD19 and CD21. Vet Immunol Immunopathol 2002; 04: 209-21.
    PubMedGoogle Scholar
  • 10 De Guise S, Erickson K, Blanchard M, Dimolfetto L, Lepper H, Wang J, Stott JL, Ferrick DS. Characterization of a monoclonal antibody that recognizes a lymphocyte surface antigen for the cetacean homologue to CD45R. Immunology 1998; 94: 207-12.
    CrossrefPubMedGoogle Scholar
  • 11 De Guise S, Erickson K, Blanchard M, Dimolfetto L, Lepper HD, Stott JL, Ferrick DA. Characterization of F21.A, a monoclonal antibody that recognizes a leukocyte surface antigen for killer whale homologue to β-2 integrin. Vet Immunol Immunopathol 2004; 97: 195-206.
    CrossrefPubMedGoogle Scholar
  • 12 Domingo M, Visa J, Pumarola M, Marco AJ, Ferrer L, Rabanal R, Kennedy S. Pathologic and immunocytochemical studies of morbillivirus infection in striped dolphins (Stenella coeruleoalba). Vet Pathol 1992; 29: 1-10.
    CrossrefPubMedGoogle Scholar
  • 13 Florez-Ramirez S, Urban-Ramirez J, Miller RD. Major histocompatibility complex class I loci from the gray whale (Eschrichtius robustus). J Hered 2000; 91: 279-82.
    CrossrefPubMedGoogle Scholar
  • 14 Hoelzel AR, Stephens JC, O’Brien SJ. Molecular genetic diversity and evolution at the MHC DQB locus in four species of pinnipeds. Mol Biol Evol 1999; 16: 611-8.
    CrossrefPubMedGoogle Scholar
  • 15 Jaber JR, Fernandez A, Herraez P, Espinosa Ade los Monteros, Ramirez GA, Garcia PM, Fernandez T, Arbelo M, Perez J. Cross-reactivity of human and bovine antibodies in striped dolphin paraffin wax-embedded tissues. Vet Immunol Immunopathol 2003; 15: 65-72.
    PubMedGoogle Scholar
  • 16 Jaber JR, Perez J, Arbelo M, Herraez P, Espinosa Ade los Monteros, Rodriguez F, Fernandez T, Fernandez A. Immunophenotypic characterization of hepatic inflammatory cell infiltrates in common dolphins (Delphinus delphis). J Comp Pathol 2003; 129: 226-30.
    CrossrefPubMedGoogle Scholar
  • 17 Jensen ED, Lipscomb T, van Bonn B, Miller G, Fradkin JM, Ridgway SH. Disseminated histoplasmosis in an atlantic bottlenose dolphin (Tursiops truncatus). J Zoo Wildl Med 1998; 29: 456-60.
    PubMedGoogle Scholar
  • 18 Jensen T, van de Bildt M, Dietz HH, Andersen TH, Hammer AS, Kuiken T, Osterhaus A. Another phocine distemper outbreak in Europe. Science 2002; 297: 209.
    CrossrefPubMedGoogle Scholar
  • 19 Kawashima M, Kuwamura M, Takeya M, Yamate J. Morphologic characteristics of pulmonary macrophages in cetaceans: particular reference to pulmonary intravascular macrophages as a newly identified type. Vet Pathol 2004; 41: 682-6.
    CrossrefPubMedGoogle Scholar
  • 20 Kawashima M, Nakanishi M, Kuwamura M, Takeya M, Yamate J. Distributive and phagocytic characteristics of hepatic macrophages in five cetaceans belonging to Delphinidae and Ziphiidae. J Vet Med Sci 2004; 66: 671-80.
    CrossrefPubMedGoogle Scholar
  • 21 Kawashima M, Nakanishi M, Kuwamura M, Takeya M, Yamate J. Immunohistochemical detection of macrophages in the short-finned pilot whale (Globicephala macrorhynchus) and Risso’s dolphin (Grampus griseus). J Comp Pathol 2004; 130: 32-40.
    CrossrefPubMedGoogle Scholar
  • 22 Kennedy S. Morbillivirus infections in aquatic mammals. J Comp Pathol 1998; 119: 201-25.
    CrossrefPubMedGoogle Scholar
  • 23 Kumar D, Cowan DF. Cross-reactivity of antibodies to human antigens with tissues of the bottlenose dolphin, Tursiops truncatus, using immunoperoxidase techniques. Mar Mamm Sci 1994; 10: 188-94.
    CrossrefPubMedGoogle Scholar
  • 24 Martineau D, Lair S, De Guise S, Lipscomb TP, Beland P. Cancer in beluga whales from the St Lawrence estuary, Quebec, Canada: a potential biomarker of environmental contamination. J Cetacean Res Manag 1999; 83: 249-65.
    PubMedGoogle Scholar
  • 25 Mikaelian I, Boisclair J, Dubey JP, Kennedy S, Martineau D. Toxoplasmosis in beluga whales (Delphinapterus leucas) from the St. Lawrence estuary: two case reports and a serological survey. J Comp Pathol 2000; 122: 73-6.
    CrossrefPubMedGoogle Scholar
  • 26 Moskov M, Schiwatschewa T, Bonev S. Vergleichshistologische Untersuchungen der Lymphknoten der Säuger. Anat Anz 1969; 124: 49-67.
    PubMedGoogle Scholar
  • 27 Müller G, Wohlsein P, Beineke A, Haas L, Greiser-Wilke I, Siebert U, Fonfara S, Harder T, Stede M, Gruber AD, Baumgartner W. Phocine distemper in German seals, 2002. Emerg Infect Dis 2004; 10: 723-5.
    CrossrefPubMedGoogle Scholar
  • 28 Murray BW, White BN. Sequence variation at the major histocompatibility complex DRB loci in beluga (Delphinapterus leucas) and narwhal (Monodon Monoceros). Immunogenetics 1998; 48: 242-52.
    CrossrefPubMedGoogle Scholar
  • 29 Rawson AJ, Patton GW, Brooks JSJ. Lymphangiomyomatosis in the atlantic bottlenose dolphin (Tursiops truncatus). J Wildl Dis 1992; 28: 323-5.
    CrossrefPubMedGoogle Scholar
  • 30 Reidarson TH, Griner LA, Pappagianis D, McBain J. Coccidioidomycosis in a bottlenose dolphin. J Wildl Dis 1998; 34: 629-31.
    CrossrefPubMedGoogle Scholar
  • 31 Romana T, Felten SY, Olschowka JA, Felten DL. A microscopic investigation of the lymphoid organs of the beluga, Delphinapterus leucas. J Morphol 1993; 215: 261-87.
    CrossrefPubMedGoogle Scholar
  • 32 Romano T, Ridgway S, Quaranta V. MHC class II molecules and immunoglobulins on peripheral blood lymphocytes of the bottlenosed dolphin, Tursiops truncatus. J Exp Zool 1992; 263: 96-104.
    CrossrefPubMedGoogle Scholar
  • 33 Romano TA, Felten SY, Olschowka JA, Felten DL. Noradrenergic and peptidergic innervation of lymphoid organs in the beluga, Delphinapterus leucas: an anatomical link between the nervous and immune systems. J Morphol 1994; 221: 243-59.
    CrossrefPubMedGoogle Scholar
  • 34 Romano TA, Ridgway SH, Felten LF, Quaranta V. Molecular cloning and characterization of CD4 in an aquatic mammal, the white whale Delphinapterus leucas. Immunogenetics 1999; 49: 376-83.
    CrossrefPubMedGoogle Scholar
  • 35 Shirai K, Sakai T, Fukuda M, Oike T. A monoclonal antibody against dolphin lymphocytes (6E9) which recognizes bovine MHC class II antigen. J Vet Med Sci 1998; 60: 291-3.
    CrossrefPubMedGoogle Scholar
  • 36 Shirai K, Sakai T, Oike T. Molecular cloning of bottle-nosed dolphin (Tursiops truncatus) MHC class I cDNA. J Vet Med Sci 1998; 60: 1093-6.
    CrossrefPubMedGoogle Scholar
  • 37 Siebert U, Müller G, Desportes G, Weiss R, Hansen K, Baumgärtner W. Pyogranulomatous myocarditis due to Staphylococcus aureus septicemia in two harbour porpoises (Phocoena phocoena). Vet Rec 2002; 150: 273-7.
    CrossrefPubMedGoogle Scholar
  • 38 Siebert U, Wünschmann A, Weiss R, Frank H, Benke H, Frese K. Post-mortem findings in harbour porpoises (Phocoena phocoena) from the German North and Baltic Sea. J Comp Pathol 2001; 124: 102-14.
    CrossrefPubMedGoogle Scholar
  • 39 Slade RW. Limited MHC polymorphism in the southern elephant seal: implications for MHC evolution and the marine mammal population biology. Proceedings of the Royal Society of London. Series B. Biol Sci 1992; 22: 163-71.
    PubMedGoogle Scholar
  • 40 Smith TL, Turnbull BS, Cowan DF. Morphology of the complex laryngeal gland in the atlantic bottlenose dolphin, Tursiops truncatus. Anat Rec 1999; 254: 98-106.
    CrossrefPubMedGoogle Scholar
  • 41 Turnbull BS, Cowan DF. Angiomatosis, a newly recognized disease in atlantic bottlenose dolphins (Tursiops truncatus) from the Gulf of Mexiko. Vet Pathol 1999; 36: 28-34.
    CrossrefPubMedGoogle Scholar
  • 42 Wünschmann A, Kremmer E, Baumgärtner W. Phenotypical characterization of T and B cell areas in lymphoid tissues of dogs with spontaneous distemper. Vet Immunol Immunopathol 2000; 73: 83-98.
    CrossrefPubMedGoogle Scholar
  • 43 Wünschmann A, Siebert U, Weiss R. Rhizopusmycosis in a harbor porpoise from the Baltic Sea. J Wildl Dis 1999; 35: 569-73.
    CrossrefPubMedGoogle Scholar
  • 44 Wünschmann A, Siebert U, Frese K. Thymic cysts in harbor porpoises (Phocoena phocoena) from the German North Sea, Baltic Sea, and Waters of Greenland. Vet Pathol 1999; 36: 391-6.
    CrossrefPubMedGoogle Scholar
  • 45 Yonezawa M, Nakamine H, Tanaka T, Miyaji T. Hodgkin’s disease in a killer whale (Orcinus orca). J Comp Pathol 1989; 100: 203-7.
    CrossrefPubMedGoogle Scholar
  • 46 Zabka TS, Romano TA. Distribution of MHC II (+) cells in the skin of the atlantic bottlenose dolphin (Tursiops truncatus): an initial investigation of dolphin dentritic cells. Anat Rec 2003; 273: 636-47.
    PubMedGoogle Scholar
  • 47 Zwillenberg HHL. Über die Milz des Braunfisches [Phocoena phocoena (L.)]. Z Anat Entwicklungsgesch 1959; 121: 9-18.
    CrossrefPubMedGoogle Scholar