Analysis of the culture-derived soluble Babesia canis canis antigens derived from the Polish strains of the parasites

L. Adaszek; A. Puchalski; M. Dec; S. Winiarczyk

doi:10.1055/s-0038-1623671

Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere, Inhaltsverzeichnis

Tierarztl Prax Ausg K Kleintiere Heimtiere 2012; 40(06): 399-403
DOI: 10.1055/s-0038-1623671

Original Article

Schattauer GmbH

Analysis of the culture-derived soluble Babesia canis canis antigens derived from the Polish strains of the parasites

Analyse des löslichen parasitären Antigens aus In-vitro-Kulturen der endemischen polnischen Stämme von Babesia canis canis

L. Adaszek

¹Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland

,

A. Puchalski

²Sub-Department of Veterinary Prevention, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland

,

M. Dec

²Sub-Department of Veterinary Prevention, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland

,

S. Winiarczyk

¹Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland

› Institutsangaben

Abstract

Summary

Objective, material and methods: The aim of this study was to analyse the protein fractions of the soluble parasitic antigen (SPA) from in vitro cultures of the native Polish strains of Babesia canis canis and to determine their immunogenicity through Western blotting using the sera of dogs vaccinated with this antigen.

Results: Polyacrylamide gel electrophoresis revealed 21 protein fractions with molecular weights from 12 to 205 kDa. The most intense reaction in Western blotting was observed between the serum antibodies of the SPA-vaccinated dogs and the fraction with the molecular weight of 52 kDa.

Conclusion: Detailed studies on the composition of SPA of Babesia canis canis and reactivity of its individual protein fractions can be a starting point for the development of subunit vaccines against babesiosis. Using a preparation with only some electrophoretic fractions of SPA in the production of vaccines would allow to avoid putting an unnecessary protein burden in the vaccine which could cause side effects.

Zusammenfassung

Ziel, Material und Methoden: Ziel dieser Studie war, aus In-vitro- Kulturen der endemischen polnischen Stämme von Babesia canis canis einzelne Proteinfraktionen des löslichen parasitären Antigens (soluble parasitic antigen, SPA) zu analysieren und deren Immunogenität im Western Blot mit den Seren der mit diesem Antigen geimpften Hunde zu bestimmen.

Ergebnisse: Durch Polyacrylamid-Gelelektrophorese ließen sich 21 Proteinfraktionen mit den Molekulargewichten von 12 bis 205 kDa isolieren. Die intensivste Reaktion in Western Blot ergab sich zwischen den Serumantikörpern der SPA-geimpften Hunde und der Fraktion mit einem Molekulargewichten von 52 kDa.

Schlussfolgerung: Detaillierte Untersuchungen zur Zusammensetzung des SPA von Babesia canis canis und zur Reaktivität der einzelnen Proteinfraktionen können als Grundlage für die Entwicklung von Subunit-Impfstoffen gegen die Babesiose des Hundes dienen. Bei Verwendung einzelner SPA- Fraktionen lassen sich belastende Fremdproteine im Impfstoff und daraus möglicherweise resultierende Nebenwirkungen bei geimpften Tieren reduzieren.

Key words

Babesia canis canis - Western blotting - SPA - dogs

Schlüsselwörter

Babesia canis canis - Western Blot - SPA - Hund

Volltext

Referenzen

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