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DOI: 10.1055/s-0038-1623671
Analysis of the culture-derived soluble Babesia canis canis antigens derived from the Polish strains of the parasites
Analyse des löslichen parasitären Antigens aus In-vitro-Kulturen der endemischen polnischen Stämme von Babesia canis canisPublication History
Received:
26 February 2012
Accepted after revision:
23 May 2012
Publication Date:
06 January 2018 (online)
Summary
Objective, material and methods: The aim of this study was to analyse the protein fractions of the soluble parasitic antigen (SPA) from in vitro cultures of the native Polish strains of Babesia canis canis and to determine their immunogenicity through Western blotting using the sera of dogs vaccinated with this antigen.
Results: Polyacrylamide gel electrophoresis revealed 21 protein fractions with molecular weights from 12 to 205 kDa. The most intense reaction in Western blotting was observed between the serum antibodies of the SPA-vaccinated dogs and the fraction with the molecular weight of 52 kDa.
Conclusion: Detailed studies on the composition of SPA of Babesia canis canis and reactivity of its individual protein fractions can be a starting point for the development of subunit vaccines against babesiosis. Using a preparation with only some electrophoretic fractions of SPA in the production of vaccines would allow to avoid putting an unnecessary protein burden in the vaccine which could cause side effects.
Zusammenfassung
Ziel, Material und Methoden: Ziel dieser Studie war, aus In-vitro- Kulturen der endemischen polnischen Stämme von Babesia canis canis einzelne Proteinfraktionen des löslichen parasitären Antigens (soluble parasitic antigen, SPA) zu analysieren und deren Immunogenität im Western Blot mit den Seren der mit diesem Antigen geimpften Hunde zu bestimmen.
Ergebnisse: Durch Polyacrylamid-Gelelektrophorese ließen sich 21 Proteinfraktionen mit den Molekulargewichten von 12 bis 205 kDa isolieren. Die intensivste Reaktion in Western Blot ergab sich zwischen den Serumantikörpern der SPA-geimpften Hunde und der Fraktion mit einem Molekulargewichten von 52 kDa.
Schlussfolgerung: Detaillierte Untersuchungen zur Zusammensetzung des SPA von Babesia canis canis und zur Reaktivität der einzelnen Proteinfraktionen können als Grundlage für die Entwicklung von Subunit-Impfstoffen gegen die Babesiose des Hundes dienen. Bei Verwendung einzelner SPA- Fraktionen lassen sich belastende Fremdproteine im Impfstoff und daraus möglicherweise resultierende Nebenwirkungen bei geimpften Tieren reduzieren.
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