Radioiodination of monoclonal antibodies, proteins and peptides for diagnosis and therapy

Th. M. Behr; M. Gotthardt; W. Becker; M. Béhé

doi:10.1055/s-0038-1625644

Nuklearmedizin - NuclearMedicine, Inhaltsverzeichnis

Nuklearmedizin 2002; 41(02): 71-79
DOI: 10.1055/s-0038-1625644

Originalarbeiten – Original Articles

Schattauer GmbH

Radioiodination of monoclonal antibodies, proteins and peptides for diagnosis and therapy

A review of standardized, reliable and safe procedures for clinical grade levels kBq to GBq in the Göttingen/Marburg Experience^[*] Radio-Iodierung von monoklonalen Antikörpern, Proteinen und Peptiden zur Diagnostik und TherapieEine Übersicht standardisierter, zuverlässiger und sicherer Verfahren im kBq- bis GBq-Bereich zum klinischen Gebrauch in der Göttingen/Marburg-Erfahrung

Autor*innen

Th. M. Behr

¹Department of Nuclear Medicine of the Philipps-University, Marburg, Göttingen, Germany
M. Gotthardt

¹Department of Nuclear Medicine of the Philipps-University, Marburg, Göttingen, Germany
W. Becker

²Department of Georg-August-University, Göttingen, Germany
M. Béhé

¹Department of Nuclear Medicine of the Philipps-University, Marburg, Göttingen, Germany

Abstract

Summary

Simple and reliable methodologies for radioiodination of proteins and peptides are described. The labeling systems are easy to assemble, capable of radioiodinating any protein or, with slight modifications, also peptide (molecular mass 1000-300,000) from kBq to GBq levels of activity for use in diagnosis and/or therapy. Furthermore, the procedures are feasible in any nuclear medicine department. Gigabecquerel amounts of activity can be handled safely. The most favored iodination methodology relies on the Iodogen system, a mild oxidating agent without reducing agents. Thus, protein degradation is minimized. Labeling yields are between 60 and 90%, and immunoreactivities remain ≥ 85%. Other radioiodination methods (chloramine-T, Bolton-Hunter) are described and briefly discussed.

Zusammenfassung

Einfache und zuverlässige Methoden zur Radioiodierung von Antikörpern, Proteinen und Peptiden mit Hilfe von Markierungssystemen werden beschrieben, die einfach und in jeder nuklearmedizinischen Abteilung zu handhaben sind. Sie sind zur Markierung von Proteinen und – mit leichten Modifikationen – auch von Peptiden geeignet, erlauben sichere Markierungen im kBqund GBq-Bereich. Das bevorzugte Verfahren beruht auf der Iodogen-Methodik, die ein mild oxidierendes Agens nutzt, so dass Proteindenaturierung weitgehend vermieden wird. Markierungsausbeuten liegen zwischen 60 und 90%, die Immunreaktivitäten liegen bei ≥85%. Andere Iodierungsmethoden (Chloramin-T, Bolton-Hunter) werden beschrieben und kurz diskutiert.

Keywords

Radioimmunotherapy - radioiodination

Schlüsselwörter

Radioimmuntherapie - Radioiodierung

Volltext

Referenzen

References
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