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Vet Comp Orthop Traumatol 1999; 12(03): 128-133
DOI: 10.1055/s-0038-1632477
Clinical Communication
Schattauer GmbH

Evaluation of a Method for Biopsy of the Distal Radial Physeal Growth Plate in Neonatal Foals

S. G. Pearce
1   From the Department of Veterinary Clinical Science (Pearce, Firth), Massey University, and AgResearch, Grasslands (Hunt), Palmerston North, New Zealand
,
E. C. Firth
1   From the Department of Veterinary Clinical Science (Pearce, Firth), Massey University, and AgResearch, Grasslands (Hunt), Palmerston North, New Zealand
,
W. F. Hunt
1   From the Department of Veterinary Clinical Science (Pearce, Firth), Massey University, and AgResearch, Grasslands (Hunt), Palmerston North, New Zealand
› Author Affiliations
Further Information

Publication History

Received 06 October 1998

Accepted 06 November 1998

Publication Date:
09 February 2018 (online)

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Summary

A technique was devised for taking biopsies from the distal radial physeal growth plate in neonatal foals to study cartilage morphology. The biopsy taken from the right forelimb of 21 Thoroughbred foals, four to 10 days of age, was performed under inhalation anaesthesia, using a 9 mm diameter trephine on a multi speed drill. Due to their exercise not being adequately restricted postoperatively, five foals (24%) developed excessive incisional granulation tissue at the biopsy site. Nine foals (43%) developed some flexion of the carpus (over at the knee) and 15 (71%) developed outward rotation of the left limb. There was significantly greater carpal valgus in the left limb when compared with the right limb (p <0.005), a situation which was not present in six agematched, unoperated, control foals. Abnormalities in the congruency of the antebrachiocarpal joint were not detected grossly at postmortem at five months of age. However, there were localised gross and histological abnormalities of the distal radial physeal growth plate. Postoperative morbidity, significant changes to conformation, and the unknown long term consequences of the disruption to the biopsied physeal growth plate are likely to make this procedure inappropriate for clinical usage. However, the biopsy procedure was easy to perform, and provided a suitable sample for both histological and histomorphometric examination of the physeal growth plate cartilage. Therefore this technique provides a useful research method for study of growth plate morphology in neonates.

A technique was developed for biopsy of the distal radial physeal growth plate in neonatal foals. Additionally, the effects on conformation, and on gross and histological abnormalities at five months of age, were reported. There were significant, mild conformational abnormalities, which self-corrected without intervention, and there were abnormalities detected at postmortem examination, but these abnormalities did not appear to affect the joint. The expected effects of this procedure are stated, and provided that these are accounted for, the technique is useful for harvesting and studying the physeal growth plate of neonates.

Keywords

Biopsy - physeal growth plate - conformation - foal
 

  • REFERENCES

  • 1 Auer JA, Martens RJ, Morris EL. Angular limb deformities in foals. Part II. Developmental factors. Comp Cont Educ Pract Vet 1983; 5: S27-S35.
    PubMedSearch in Google Scholar
  • 2 Belling TH, Glade MJ. A nondestructive biopsy method allowing the rapid removal of live growth-plate cartilage. Vet Med 1984; 528-31.
    PubMedSearch in Google Scholar
  • 3 Carlson CS, Cullins LD, Meutens DJ. Osteochondrosis of the articular-epiphyseal cartilage complex in young horses: evidence for a defect in cartilage canal blood supply. Vet Pathol 1995; 32: 641-7.
    CrossrefPubMedSearch in Google Scholar
  • 4 Craig E. Autogenous fat grafts to prevent recurrence following surgical correction of growth deformities of the radius and ulna in the dog. Vet Surg 1981; 10: 69-73.
    CrossrefPubMedSearch in Google Scholar
  • 5 Duff SRI. Morphological and radiological findings in induced abnormal bone growth in lambs. J Comp Path 1986; 96: 3-13.
    CrossrefPubMedSearch in Google Scholar
  • 6 Firth EC. Poulos PW. Retained cartilage in the distal radial physis of foals. Vet Pathol 1984; 21: 10-17.
    CrossrefPubMedSearch in Google Scholar
  • 7 Fretz PB. Angular limb deformities in foals. Vet Clin North Am 1980; 2: 125-50.
    CrossrefPubMedSearch in Google Scholar
  • 8 Jeffcott LB. Osteochondrosis in the horse - searching for the key to pathogenesis. Equine Vet J 1991; 23: 331-8.
    CrossrefPubMedSearch in Google Scholar
  • 9 Hunt WF, Thomas VG, Stiefel W. Video camera recorded image analysis to determine linear and angular dimensions in the growing horse. In preparation.
    PubMed
  • 10 Lillich- JD, Bertone AL, Malemud CJ, Weisbrode SE, Ruggles AJ, Stevenson S. Biochemical, histochemical, and immunohistochemical characterization of distal tibial osteochondrosis in horses. Am J Vet Res 1997; 58: 89-98.
    PubMedSearch in Google Scholar
  • 11 Misheff MM, Stover SM, Pool RR. Corticocancellous bone biopsy from the 12th rib of standing horses. Vet Surg 1992; 21: 133-8.
    CrossrefPubMedSearch in Google Scholar
  • 12 O'Donohue DD, Smith FH, Strickland KL. The incidence of abnormal limb development in the Irish Thoroughbred from birth to 18 months. Equine Vet J 1992; 24: 303-9.
    PubMedSearch in Google Scholar
  • 13 Savage CJ, Jeffcott LB, Melsen F. et al Bone biopsy in the horse 1. Method using the wing of the ilium. J Vet Med 1991; 38: 776-83.
    CrossrefPubMedSearch in Google Scholar
  • 14 Savage CJ, McCarthy RN, Jeffcott LB. Histomorphometric assessment of bone biopsies from foals fed diets high in phosphorus and digestible energy. Equine Vet J 1993; Suppl 16: 89-93.
    PubMedSearch in Google Scholar