An Animal Model for Research on Wound Healing over Exposed Bone

D. M. Bradley; S. F. Swaim; S.W. Stuart

doi:10.1055/s-0038-1632534

Veterinary and Comparative Orthopaedics and Traumatology, Inhaltsverzeichnis

Vet Comp Orthop Traumatol 1998; 11(03): 131-135
DOI: 10.1055/s-0038-1632534

Original Research

Schattauer GmbH

An Animal Model for Research on Wound Healing over Exposed Bone

D. M. Bradley

¹Wound Healing/Reconstructive Surgery Program, Scott-Ritchey Research Center, Auburn University College of Veterinary Medicine, Auburn University, AL, USA

,

S. F. Swaim

¹Wound Healing/Reconstructive Surgery Program, Scott-Ritchey Research Center, Auburn University College of Veterinary Medicine, Auburn University, AL, USA

,

S.W. Stuart

¹Wound Healing/Reconstructive Surgery Program, Scott-Ritchey Research Center, Auburn University College of Veterinary Medicine, Auburn University, AL, USA

› Institutsangaben

Abstract

Summary

Many wounds such as degloving injuries, or dermal ulcerative processes may have exposed bone. This presents a problem in wound healing. Various procedures such as tissue flaps, skin expansion, skin grafts, or open wound healing may be used to treat wounds with exposed bone. The development of a healthy granulation tissue bed provides the supportive structural matrix and vasculature needed to promote wound healing over exposed bone. This is beneficial when flaps or expanded skin are used and is necessary if skin grafts or open wound healing are used for treatment. This paper describes an animal model and surgical technique that can be used in studying factors that might enhance wound healing over exposed bone. A technique is described whereby a rectangular defect with exposed bone is made over the second metatarsal bone of New Zealand white rabbits. Using subjective observations and objective planimetry, the rate of healing can be evaluated. The animal model and technique provide a means of evaluating effects of medications and bandages on the healing of wounds with exposed bone. The model could also be considered a model for wounds with delayed healing.

This paper describes an animal model and surgical technique that can be used to study wound healing over exposed bone. The surgical technique involves the creation of a rectangular defect over the second metatarsal bone of New Zealand White rabbits, with removal of the overlying periosteum. Various topical medications and/or bandage materials can be evaluated as to their effects on the healing of wounds with exposed bone. Bilateral defects can be created, with the animal serving as its own control. Subjective and objective evaluations involving planimetry, histopathologic, biochemical, or immunohistochemical analyses of harvested tissues may be conducted.

Key words

New Zealand white rabbits - wound healing - exposed bone - animal model - granulation tissue

Volltext

Referenzen

REFERENCES
1 Ashhurst DE, Hogg J, Perren SM. A method for making reproducible experimental fractures of the rabbit tibia. Injury 1982; 14: 236-42.
2 Beardsley SL, Schrader SC. Treatment of dogs with wounds of the limbs caused by shearing forces: 98 cases (1975-1993). J Am Med Assoc 1995; 207 1071;
3 Cabbabe EB, Korock SW, Malik PA. Skin grafting denuded skull. Ann Plastic Surg 1982; 8: 318-21.
4 Davison TC. Replacement of scalp on a denuded dry skull by granulations secured through holes drilled in the bone. J Am Med Assoc 1918; 70: 1368.
5 Feierabend T, Bindra R. Injuries causing major loss of scalp. Plast Reconstr Surg 1985; 76: 189-94.
6 Gangjee T, Colaizzo R, von Recum AF. Species-related differences in percutaneous wound healing. Ann Biomed Eng 1985; 13: 451-67.
7 Haalboom JRE, van Everdingen JJE, Cullum N. Incidence, prevalence, and classification. In The Decubitus Ulcer in Clinical Practice.. Parish LC, Witkowski JA, Crissey JT. (eds). New York: Springer; 1997: 12-23.
8 Howard LL, Armstrong TM, Smith D. Avulsions of the scalp with plastic repair. Am J Surg 1950; 80: 714-7.
9 Hurwitz DJ. Osseous interference of soft tissue healing. In Surgical Clinics of North America.. Romm S. (ed). Philadelphia: W. B. Saunders; 1984: 699-704.
10 Kazanjian VH, Webster RC. The treatment of extensive losses of the scalp. Plastic Reconstr Surg 1946; 1: 360-85.
11 Lee AH, Swaim SF, Newton JC. et al. Wound healing over denuded bone. J Am Anim Hosp Assoc 1987; 23: 75-84.
12 McGregor IA. Fundamental Techniques of Plastic Surgery. 8th ed. New York: Churchill Livingstone; 1989: 155-68.
13 Osaki K, Miura A, Koide M, Katakura T, Sugihara T. The effects of a collagenous implant on deep dermo-periosteal defects in the rabbit. J Derm 1996; 23: 83-8.
14 Pavletic MM. Atlas of Small Animal Reconstructive Surgery. Philadelphia: JB Lippincott Co.; 1993: 38-64.
15 Robertson J. Remarks on management of the scalped head. In Surgery in America: From the Colonial Era to the Twentieth Century. 2^nd ed. Earle AS. (ed). New York: Praeger Publishers; 1983: 36-40.
16 Rudolph R, Vande Berg J, Ehrlich HP. Wound contraction and scar contracture. In Wound Healing: Biochemical and Clinical Aspects.. Cohen IK, Diegelmann RF, Lindblad WJ. (eds.) Philadelphia: W. B. Saunders; 1992: 96-114.
17 Stashak TS. Equine Wound Management. Philadelphia: Lea and Febiger; 1991: 163-217.
18 Strayer LM. Augustin Belloste and the treatment for avulsion of the scalp. New Eng J Med 1939; 220: 901-5.
19 Stuart SW. A new rabbit handling and restraint technique. Lab Anim 1997; 26: 30-1.
20 Stuzin JM, Zide BM. Scalp, calvarium, and forehead reconstruction. In Grabb and Smith's Plastic Surgery. 4th ed.. Smith JW, Aston SJ. (eds). Boston: Little, Brown and Company; 1991: 397-423.
21 Swaim SF, Pope ER. Early management of limb degloving injuries. Sem Vet Med Surg (Small Anim) 1988; 3: 274-81.
22 Swaim SF, Hanson Jr. RR, Coates JR. Pressure wounds in animals. Compend Contin Educ 1996; 18: 203-19.
23 Swaim SF, Henderson RA. Small Animal Wound Management. 2^nd ed. Media PA: Williams and Wilkins; 1997: 305-6.
24 Thompson LW, Spivack J, Bennett JE. Management of large scalp defects. J Trauma 1970; 10: 153-62.