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Vet Comp Orthop Traumatol 2000; 13(04): 178-184
DOI: 10.1055/s-0038-1632657
Original Research
Schattauer GmbH

The presence of collagen types II and X in medial coronoid processes of 21 dogs

D. T. Crouch
1   Comparative Orthopaedic Laboratory, University of Missouri- Columbia, Columbia, MO, USA
,
J. L. Cook
1   Comparative Orthopaedic Laboratory, University of Missouri- Columbia, Columbia, MO, USA
,
D. D. Lewis
2   The Department of Small Animal Clinical Sciences and the Centre for Veterinary Sports Medicine, University of Florida, Gainesville, FL, USA
,
J. M. Kreeger
1   Comparative Orthopaedic Laboratory, University of Missouri- Columbia, Columbia, MO, USA
,
J. L. Tomlinson
1   Comparative Orthopaedic Laboratory, University of Missouri- Columbia, Columbia, MO, USA
› Author AffiliationsThe authors thank Dr. Gary Gibson from Henry Ford Hospital for generously supplying the collagen type X antibody used in this study and for his help with the immunohistochemistry technique.
Further Information

Publication History

Received 05 April 2000

Accepted 25 June 2000

Publication Date:
09 February 2018 (online)

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Summary

The purpose of this study was to determine collagen type II and X immunohistochemical staining characteristics of naturally-occurring fragmented medial coronoid processes (FMCP) in order to help delineate potential pathophysiological events associated with FMCP.

34 surgically excised FMCP from 21 client-owned dogs and 16 intact medial coronoid processes from 8 mongrel dogs were examined. The specimens were categorized by the dog's age: <12 months, 12–18 months, 18–24 months, >24 months.

The excised FMCP and normal medial coronoid processes were sectioned and stained with haematoxylin and eosin, safranin-O fast green, and toluidine blue. Sections were subjectively evaluated for tissue morphology, cell and matrix content, and proteoglycan staining. Immunohistochemical staining was performed for collagen types II and X. Sections were then subjectively evaluated for the location and intensity of staining.

FMCP tissue demonstrated a wide variety of histological and immunohistochemical characteristics compared to normal medial coronoid processes. Significantly (P = 0.016) more normal dogs stained positive for collagen type X than dogs with FMCP in the <12 months old group. No other significant differences between affected and normal dogs were noted for either collagen type in any age group. No significant difference in age was noted for the presence or absence of collagen type II among affected dogs, and no statistically significant correlation was observed between age of those with collagens type X and type II present. Although not statistically significant (P = 0.100), there was a trend for the presence of collagen type II when collagen type X was present. The results of this study have not provided a definitive answer regarding the role of collagen type X in the etiopathogenesis of FMCP, but suggest that it may be an important factor in some cases, warranting further investigation.

Excised fragmented medial coronoid processes were examined to determine the collagen immunohistochemical staining characteristics when compared to intact processes.

Keywords

Fragmented medial coronoid process - collagen type II - collagen type X - immunohistochemistry
 

  • REFERENCES

  • 1 Alini M, Kofsky Y, Wu W. et al. In serumfree culture thyroid hormones can induce full expression of chondrocyte hypertrophy leading to matrix calcification. J Bone Miner Res 1996; 11: 105-13.
    PubMedGoogle Scholar
  • 2 Boulay JP. Fragmented medial coronoid process of the ulna in the dog. Vet Clin N Amer 1998; 28 (01) 51-74.
    PubMedGoogle Scholar
  • 3 Buckwalter JA, Mankin HJ. Articular cartilage: tissue design and chondrocyte-matrix interactions. Instructional Course Lectures 1998; 47: 477-86.
    PubMedGoogle Scholar
  • 4 Collins KE, Cross AR, Lewis DD. et al. A comparison of the radius of curvature of the ulnar and trochlear notch of Rottweilers and Greyhounds using three dimensional digitization, in Proceedings. 27th Annu Vet Surg Forum 1999: 4.
    PubMedGoogle Scholar
  • 5 Denny HR, Gibbs C. The surgical treatment of osteochondritis dissecans and ununited coronoid process in the canine elbow joint. J Small Anim Pract 1980; 21: 323-31.
    CrossrefPubMedGoogle Scholar
  • 6 Fox SM, Bloomberg MS, Bright RM. Developmental anomalies of the canine elbow. J Am Anim Hosp Assoc 1983; 19: 605-15.
    PubMedGoogle Scholar
  • 7 Girkontaite I, Frischholz S, Lammi P. et al. Immunolocalization of type X collagen in normal fetal and adult osteoarthritic cartilage with monoclonal antibodies. Mat Bio 1996; 15 (04) 231-8.
    PubMedGoogle Scholar
  • 8 Grøndalen J. Arthrosis of the elbow joint of young rapidly growing dogs (V). A. pathoanatomical investigation. Nord Vet Med 1981; 33: 1-16.
    PubMedGoogle Scholar
  • 9 Guthrie S, Plummer J, Vaughan L. Post natal development of the canine elbow joint: A light and electron microscopical study. ‘Res Vet Sci 1992; 52: 67-71.
    PubMedGoogle Scholar
  • 10 Guthrie S, Plummer J, Vaughan L. Aetiopathogenesis of canine elbow osteochondrosis: A study of loose fragments removed at arthrotomy. Res Vet Sci 1992; 52: 284-91.
    CrossrefPubMedGoogle Scholar
  • 11 Hare WCD. The ages at which the centres of ossification appear roentgenographically in the limb bones of the dog. Am J Vet Res 1961; 22: 825-35.
    Google Scholar
  • 12 Iyama K-I, Ninomiya Y, Olsen BR. et al. Spatiotemporal pattern of type X collagen gene expression and collagen deposition in embryonic chick vertebrae undergoing endochondral ossification. Anat Rec 1991; 229: 462-72.
    CrossrefPubMedGoogle Scholar
  • 13 Kuivaniemi H, Tromp G, Prockop DJ. Mutations in fibrillar collagens (types I, II, III, XI), fibril-associated collagen (type IX), and network-forming collagen (type X ) cause a spectrum of diseases of bone, cartilage, and blood vessels. Hum Mut 1997; 09 (04) 300-15.
    CrossrefPubMedGoogle Scholar
  • 14 Kwan KM, Pang MK, Zhou S. et al. Abnormal compartmentalization of cartilage matrix components in mice lacking collagen X: Implications for function. J Cell Bio 1997; 136 (02) 459-71.
    CrossrefPubMedGoogle Scholar
  • 15 Lewis DD, Parker RB, Hager DA. Fragmented medial coronoid process of the canine elbow. Compend Contin Educ Pract Vet 1989; 11: 703-16.
    PubMedGoogle Scholar
  • 16 Linsenmayer TF, Chen Q, Gibney E. et al. Collagen types IX and X in the developing chick tibiotarsus: analysis of mRNAs and proteins. Development 1991; 111: 91-6.
    PubMedGoogle Scholar
  • 17 Long F, Linsenmayer TF. Regulation of growth region cartilage proliferation and differentiation by perichondrium. Development 1998; 125 (06) 1067-73.
    PubMedGoogle Scholar
  • 18 Long F, Sonenshein GE, Linsenmayer TF. Multiple transcriptional elements in the avian type X collagen gene. Identification of Spl family proteins as regulators for high level expression in hypertrophic chondrocytes. J Bio Chem 1998; 273 (11) 6542-9.
    CrossrefPubMedGoogle Scholar
  • 19 LuValle P, Hayashi M, Olsen BR. Transcriptional regulation of type X collagen during chondrocyte maturation. Dev Bio 1989; 133: 613-6.
    CrossrefPubMedGoogle Scholar
  • 20 Morrison EH, Ferguson MW, Bayliss MT. et al. The development of articular cartilage: The spatial and temporal patterns of collagen types. J Anat 1996; 189 (Pt 1): 9-22.
    PubMedGoogle Scholar
  • 21 Muir H. The chondrocyte, architect of cartilage. Biomechanics, structure, function and molecular biology of cartilage matrix macromolecules. Bioessays 1995; 17 (12) 1039-48.
    CrossrefPubMedGoogle Scholar
  • 22 Olsson SE. A new type of elbow dysplasia in the dog. A preliminary report. Svensk Vet tidn 1974; 05: 152-7.
    PubMedGoogle Scholar
  • 23 Olsson SE. Lameness in the dog. A review of lesions causing osteoarthritis of the shoulder, elbow, hip, stifle and hock joints. Am Anim Hosp Assoc Proc 1975; 01: 363.
    PubMedGoogle Scholar
  • 24 Olsson SE. The early diagnosis of fragmented coronoid process and osteochondritis dissecans of the canine elbow joint. J Am Anim Hosp Assoc 1983; 19: 616-26.
    PubMedGoogle Scholar
  • 25 Oshima O, Leboy PS, McDonald SA. et al. Developmental expression of genes in chick growth cartilage detected by in situ hybridization. Calcif Tiss Int 1989; 45: 182-92.
    CrossrefPubMedGoogle Scholar
  • 26 Poole AR, Pidoux I. Immunoelectron microscopic studies of type X collagen in endochondral ossification. J Cell Biol 1989; 109: 2547-54.
    CrossrefPubMedGoogle Scholar
  • 27 Poole AR. Cartilage macromolecules and the calcification of cartilage matrix. Anat Rec 1989; 224: 167.
    CrossrefPubMedGoogle Scholar
  • 28 Presnell KR. Surgical Treatment of Fragmented Medial Coronoid Process. In: Bojrab MJ, Ellison GW, Slocum B. ed 4. Current Techniques in Small Animal Surgery. Philadelphia: Williams and Wilkens; 1998: 1090-4.
    Google Scholar
  • 29 Preston CA, Schulz KS. Articular contact areas in the canine elbow joint, in Proceedings. 27th Annu Vet Surg Forum. 1999: 17.
    PubMedGoogle Scholar
  • 30 Rucklidge GJ, Milne G, Robins SP. Collagen type X: a component of the surface of normal human, pig, and rat articular cartilage. Biochem Biophys Res Comm 1996; 224 (02) 297-302.
    CrossrefPubMedGoogle Scholar
  • 31 Sasaki T, Kim TW, Debari K. et al. Cartilage-bone replacement in endochondral ossification of mandibular condylar heads in young beagles. J Electron Micro 1996; 45 (03) 213-22.
    PubMedGoogle Scholar
  • 32 Schmid TM, Conrad HE. A unique low molecular weight collagen secreted by cultured chick embryo chondrocytes. J Biol Chem 1982; 257: 12444-50.
    PubMedGoogle Scholar
  • 33 Schmid TM, Linsenmayer TF. Immunoelectron microscopy of type X collagen. Supra-molecular forms within embryonic chick cartilage. Dev Biol 1990; 138: 52-62.
    PubMedGoogle Scholar
  • 34 Wind AP. Elbow incongruity and developmental elbow diseases in the dog: Part I. J Am Anim Hosp Assoc 1986; 22: 711.
    PubMedGoogle Scholar
  • 35 Wind AP. Elbow incongruity and developmental elbow diseases in the dog: Part II. J Am Anim Hosp Assoc 1986; 22: 724.
    PubMedGoogle Scholar
  • 36 Wind AP. Elbow Dysplasia. In: Slatter D. (ed 2. Textbook of Small Animal Surgery. Philadelphia: WB Saunders Co; 1993: 1966-77.
    Google Scholar
  • 37 Wosar MA, Lewis DD, Neuwirth L. et al. Radiographic evaluation of elbow joints before and after surgery in dogs with possible fragmented medial coronoid process. J Am Vet Med Assoc 1999; 214 (01) 52-8.
    PubMedGoogle Scholar