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Vet Comp Orthop Traumatol 2006; 19(03): 133-141
DOI: 10.1055/s-0038-1632989
Review Article
Schattauer GmbH

Osteoinductive properties of the bone marrow Myth or reality

M. Tshamala
1   Departments of Medicine and Clinical Biology of Small Animals
2   Diagnostic Imaging of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
,
H. van Bree
2   Diagnostic Imaging of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
› Institutsangaben
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Publikationsverlauf

Received 04. Juli 2005

Accepted 29. Januar 2006

Publikationsdatum:
08. Februar 2018 (online)

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Summary

The osteogenic potential of red bone marrow was first reported more than 100 years ago. Since then, studies have reported controversial results that do not confirm nor disprove the capacity of fresh red bone marrow to produce bone. Researches have been focused on techniques that improve the efficiency of the bone marrow, including: the increase of the concentration of the mesenchymal stem cells in the aspirated bone marrow, the combination with a ‘carrier’ that helps to maintain the mesenchymal stem cells and guides and supports the vascular ingrowth in the defect, or the combination with bone growth factors that stimulate the marrow stromal cells to differentiate into bone forming cells. Each of these techniques has its drawbacks and increases the expenses of an operation. On the other hand, the synergistic effect observed with these combinations does not resolve the problem of the osteogenic capacity of pure bone marrow, which still remains questionable.

Keywords

Bone marrow - osteogenesis - osteoinduction - ceramics - growth factors
 

  • References

  • 1 Basset CAL. Clinical implications of cell function in bone grafting. Clin Orthop 1972; 87: 49-59.
    PubMedSuche in Google Scholar
  • 2 Tiedeman JJ, Connolly JF, Strates BS. et al. Treatment of nonunion by percutaneous injection of bone marrow and demineralised bone matrix. An experimental study in dogs. Clin Orthop 1991; 268: 294-302.
    PubMedSuche in Google Scholar
  • 3 Sasaki T, Watanabe C. Stimulation of osteoinduction in bone wound healing by high-molecular hyaluronic acid. Bone 1995; 16: 9-15.
    CrossrefPubMedSuche in Google Scholar
  • 4 Lane JM, Yasko AW, Tomin E. et al. Bone marrow and recombinant human BMP-2 in osseous repair. Clin Orthop 1999; 361: 216-27.
    CrossrefPubMedSuche in Google Scholar
  • 5 den Boer FC, Wippermann BW, Blokhuis TJ. et al. Healing of segmental bone defects with granular porous hydroxyapatite augmented with recombinant human osteogenic protein-1 or autologous bone marrow. J Orthop Res 2003; 21: 521-8.
    CrossrefPubMedSuche in Google Scholar
  • 6 Andrades JA, Han B, Becera J. et al. A recombinant human TGF-beta 1 fusion protein with collagen-binding domain promotes migration, growth, and differentiation ofbone marrow mesenchymal cells. Exp Cell Res 1999; 250: 485-98.
    CrossrefPubMedSuche in Google Scholar
  • 7 Aubin JE. Osteoprogenitor cell frequency: Role for heterotypic cell-cell interactions in osteoblast differentiation. J Cell Biochem 1999; 72: 396-410.
    CrossrefPubMedSuche in Google Scholar
  • 8 Grundel RE, Chapman MW, Yee T. et al. Autogeneic bone marrow and porous biphasic calcium phosphate ceramic for segmental bone defects in the canine ulna. Clin Orthop 1991; 266: 244-58.
    PubMedSuche in Google Scholar
  • 9 Muschler GF, Nitto H, Boehm CA. et al. Age-and gender-related changes in the cellularity of human bone marrow and the prevalence of osteoblast progenitors. J Orthop Res 2001; 19: 117-25.
    CrossrefPubMedSuche in Google Scholar
  • 10 Werntz JR, Lane JM, Burstein AH. et al. Qualitative and quantitative analysis of orthotopic bone regeneration by marrow. J Orthop Res 1996; 14: 85-93.
    CrossrefPubMedSuche in Google Scholar
  • 11 Deans RJ, Moseley AB. Mesenchymal stem cells: Biology and potential clinical uses. Exp Hematol 2000; 28: 875-84.
    CrossrefPubMedSuche in Google Scholar
  • 12 Muschler GF, Boehm C, Easley K. Aspiration to obtain osteoblast progenitor cells from human bone marrow: The influence of aspiration volume. J Bone Joint Surg Am 1997; 79-A: 1699-709.
    PubMedSuche in Google Scholar
  • 13 Muschler GF, Midura RJ. Connective tissue progenitors: Practical concepts for clinical applications. Clin Orthop 2002; 395: 66-80.
    CrossrefPubMedSuche in Google Scholar
  • 14 Connolly JF. Injectable bone marrow preparations to stimulate osteogenic repair. Clin Orthop 1995; 313: 8-18.
    PubMedSuche in Google Scholar
  • 15 Devine MJ, Mierisch CM, Jang E. et al. Transplanted bone marrow cells localize to fracture callus in a mouse model. J Orthop Res 2002; 20: 1232-9.
    CrossrefPubMedSuche in Google Scholar
  • 16 Majors AK, Boehm CA, Nitto H. et al. Characterization of human bone marrow stromal cells with respect to osteoblastic differentiation. J Orthop Res 1997; 15: 546-57.
    CrossrefPubMedSuche in Google Scholar
  • 17 Ekelund A, Brosjö O, Nilsson OS. Experimental induction of heterotopic bone. Clin Orthop 1991; 263: 102-12.
    PubMedSuche in Google Scholar
  • 18 Gronthos S, Simmons PJ, Graves SE. et al. Integrin-mediated interactions between human bone marrow stromal precursor cells and the extracellular matrix. Bone 2001; 28: 174-81.
    CrossrefPubMedSuche in Google Scholar
  • 19 Ongpipattanakul B, Nguyen T, Zioncheck TF. et al. Development of tricalcium phosphate/amylopectin paste combined with recombinant human transforming growth factor beta 1 as abone defect filler. J Biomed Mater Res 1997; 36: 295-305.
    CrossrefPubMedSuche in Google Scholar
  • 20 Tagaki K, Urist M. The role of bone marrow in bone morphogenetic protein-induced repair of femoral massive diaphyseal defects. Clin Orthop 1982; 171: 224-31.
    PubMedSuche in Google Scholar
  • 21 Burwell R.G.. The function of bone marrowinthe incorporation of a bone graft. Clin Orthop 1985; 200: 125-41.
    PubMedSuche in Google Scholar
  • 22 Nade S. Stimulating osteogenesis. Injury 1994; 25: 577-83.
    CrossrefPubMedSuche in Google Scholar
  • 23 Connolly JF, Guse R, Tiedeman J. et al. Autologous marrow injection as a substitute for operative grafting of tibial nonunions. Clin Orthop 1991; 266: 259-70.
    PubMedSuche in Google Scholar
  • 24 Curylo LJ, Johnstone B, Petersilge CA. et al. Augmentation of spinal arthrodesis with autologous bone marrow in a rabbit posterolateral spine fusion model. Spine 1999; 24: 434-8.
    CrossrefPubMedSuche in Google Scholar
  • 25 Boden SD, Martin GJ, Morone M. et al. The use of coralline hydroxyapatite with bone marrow, autogenous bone graft, or osteoinductive bone protein extract for posterolateral lumbar spine fusion. Spine 1999; 24: 320-7.
    CrossrefPubMedSuche in Google Scholar
  • 26 Cinotti G, Patti AM, Vulcano A. et al. Experimental posterolateral spinal fusion with porous ceramics and mesenchymal stem cells. J Bone Joint Surg Br 2004; 86-B: 135-42.
    CrossrefPubMedSuche in Google Scholar
  • 27 Wippermann B, Donow C, Schratt HE. et al. The influence of hydroxyapatite granules on the healing of a segmental defect filled with autologous bone marrow. Ann Chir Gynaecol 1999; 88: 194-7.
    PubMedSuche in Google Scholar
  • 28 Egrise D, Martin D, Vienne A. et al. The number of fibroblastic colonies formed from bone marrow is decreased and the in vitro proliferation rate of trabecular bone cells increased in aged rats. Bone 1992; 13: 355-61.
    CrossrefPubMedSuche in Google Scholar
  • 29 Krebsbach PH, Kuznetsov SA, Satomura K. et al. Bone formation in vivo: Comparison of osteogenesis by transplanted mouse and human marrow stromal fibroblasts. Transplantation 1997; 63: 1059-69.
    CrossrefPubMedSuche in Google Scholar
  • 30 Tsuchida H, Hashimoto J, Crawford E. et al. Engineered allogeneic mesenchymal stem cells repair femoral segmental defect in rats. J Orthop Res 2003; 21: 44-53.
    CrossrefPubMedSuche in Google Scholar
  • 31 Arinzeh TL, Peter SJ, Archambault MP. et al. Allogeneic mesenchymal stem cells regenerate bone in a critical-sized canine segmental defect. J Bone Joint Surg Am 2003; 85-A: 1927-35.
    PubMedSuche in Google Scholar
  • 32 Stenderup K, Justesen J, Eriksen EF. et al. Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis. JBone Miner Res 2001; 16: 1120-9.
    CrossrefPubMedSuche in Google Scholar
  • 33 Bruder SP, Jaiswal N, Haynesworth SE. Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J Cell Biochem 1997; 64: 278-94.
    CrossrefPubMedSuche in Google Scholar
  • 34 Bruder SP, Fink DJ, Caplan AI. Mesenchymal stem cells in bone formation, bone repair, and skeletal regeneration therapy. J Cell Biochem 1994; 56: 283-94.
    PubMedSuche in Google Scholar
  • 35 Connolly JF, Guse R, Tiedeman J. et al. Autologous marrow injection for delayed unions of the tibia – a preliminary report. J Orthop Trauma 1989; 3: 276.
    CrossrefPubMedSuche in Google Scholar
  • 36 Simmons PJ, Torok-Storb B. Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood 1991; 78: 55-62.
    PubMedSuche in Google Scholar
  • 37 Joyner CJ, Bennett A, Triffitt JT. Identification and enrichment of human osteoprogenitor cells by differentiation stage-specific monoclonal antibodies. Bone 1997; 21: 1-6.
    CrossrefPubMedSuche in Google Scholar
  • 38 Herbertson A, Aubin JE. Cell sorting enriches osteogenic populations in rat bone marrow stromal cell cultures. Bone 1997; 21: 491-500.
    CrossrefPubMedSuche in Google Scholar
  • 39 Martin GJ, Boden SD, Titus L. et al. New formulations of demineralised bone matrix as amore effective graft alternative in experimental posterolateral lumbar spine arthrodesis. Spine 1999; 24: 637-45.
    CrossrefPubMedSuche in Google Scholar
  • 40 Yuan HP, De Bruijn JD, Zhang XD. et al. Use of an osteoinductive biomaterial as a bone morphogenetic protein carrier. J Mater Sci Mater Med 2001; 12: 761-6.
    CrossrefPubMedSuche in Google Scholar
  • 41 Kadiyala S, Jaiswal N, Bruder SP. Culture-expanded, bone marrow-derived mesenchymal stem cells can regenerate a critical-sized segmental bone defect. Tissue Eng 1997; 3: 173-85.
    CrossrefPubMedSuche in Google Scholar
  • 42 Kuboki Y, Takita H, Koboyashi D. et al. BMP-induced osteogenesis on the surface of hydroxyapatite with geometrically feasible and nonfeasible structures: Topology of osteogenesis. J Biomed Mater Res 1998; 39: 190-9.
    CrossrefPubMedSuche in Google Scholar
  • 43 Oonishi H, Hench LL, Wilson Jetal. Comparative bone growth behavior in granules of bioceramic materials of various sizes. J Biomed Mater Res 1999; 44: 31-43.
    CrossrefPubMedSuche in Google Scholar
  • 44 Walsh WR. et al. A resorbable porous ceramic composite bone graft substitute in a rabbit metaphyseal defect model. J. Orthop Res 2003; 21: 655-61.
    CrossrefPubMedSuche in Google Scholar
  • 45 Mankani MH, Kuznetsov SA, Fowler B. et al. In vivo bone formation by human bone marrow stromal cells: Effect of carrier particle size and shape. Biotechnol Bioeng 2001; 72: 96-107.
    CrossrefPubMedSuche in Google Scholar
  • 46 Wozney JM. The bone morphogenetic protein family and osteogenesis. Mol Reprod Dev 1992; 32: 160-7.
    CrossrefPubMedSuche in Google Scholar
  • 47 Yasko AW, Lane JM, Fellinger EJ. et al. The healing of segmental bone defects, induced by recombinant human bone morphogenetic protein (RHBMP-2) – A radiographic, histological, and biomechanical study in rats. J Bone and Joint Surg Am 1992; 74-A: 659-70.
    PubMedSuche in Google Scholar
  • 48 Itoh H, Ebara S, Kamimura M. et al. Experimental spinal fusion with use of recombinant human bone morphogenetic protein 2. Spine 1999; 24: 1402.
    CrossrefPubMedSuche in Google Scholar
  • 49 Cheng H, Jiang W, Phillips FM. et al. Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J Bone Joint Surg Am 2003; 85-A: 1544-52.
    CrossrefPubMedSuche in Google Scholar
  • 50 Clarke SA, Brooks RA, Lee PTH. et al. Bone growth into ceramic-filled defect around an implant. J Bone Joint Surg Br 2004; 86-B: 126-34.
    CrossrefPubMedSuche in Google Scholar
  • 51 Buckwalter JA, Glimcher MJ, Cooper RR. et al. Bone biology. Part II: Formation, form, modelling, remodelling, and regulation of cell function. J Bone Joint Surg Am 1995; 77-A: 1276-89.
    PubMedSuche in Google Scholar
  • 52 Sellers RS, Peluso D, Morris EA. The effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on the healing of full-thickness defect of articular cartilage. J Bone Joint Surg Am 1997; 79-A: 1452-63.
    PubMedSuche in Google Scholar
  • 53 Beck LS, Wong RL, DeGuzman L. et al. Combination ofbone marrow and TGF-beta1 augment the healing of critical-sized bone defects. J Pharm Sci 1998; 87: 1379-86.
    CrossrefPubMedSuche in Google Scholar