Expression of LOXs and MMP-1, 2, 3 by ACL Fibroblasts and Synoviocytes Impact of Coculture and TNF-α

Chunli Wang; Qingjia Chi; Chunming Xu; Kang Xu; Yanjun Zhang; Yan Liu; Li Yang; K. L. Paul Sung

doi:10.1055/s-0038-1641592

The Journal of Knee Surgery, Inhaltsverzeichnis

J Knee Surg 2019; 32(04): 352-360
DOI: 10.1055/s-0038-1641592

Original Article

Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Expression of LOXs and MMP-1, 2, 3 by ACL Fibroblasts and Synoviocytes Impact of Coculture and TNF-α

Autor*innen

Chunli Wang

¹“111” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, Republic of China
Qingjia Chi

²Department of Mechanics and Engineering Structure, Hubei Key Laboratory of Theory and Application of Advanced Materials Mechanics, Wuhan University of Technology, Wuhan, Hubei, Republic of China
Chunming Xu

¹“111” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, Republic of China
Kang Xu

¹“111” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, Republic of China
Yanjun Zhang

⁴Department of Life Science, Hunan University of Science and Technology, Xiangtan, Hunan, Republic of China
Yan Liu

¹“111” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, Republic of China
Li Yang

¹“111” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, Republic of China
K. L. Paul Sung

¹“111” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, Republic of China

³Department of Bioengineering and Orthopaedics, University of California, San Diego, La Jolla, California

Abstract

This study aims to confirm the effects of synoviocytes (SCs) on regulating lysyl oxidases (LOXs) and matrix metalloproteinase (MMP)-1, 2, 3 in the normal and injured anterior cruciate ligament (ACL) fibroblasts response to tumor necrosis factor-α(TNF-α). The gene and protein expression levels of LOXs and MMP-1, 2, 3 in SCs cocultured ACL fibroblasts (ACLfs) induced by TNF-α and mechanical injury were analyzed by real-time polymerase chain reaction (PCR) and western bolting; the MMP-2 activity were analyzed by zymography. The results exhibited that TNF-α alone slightly downregulated the expressions of LOXs and upregulated the expression of MMP-1, 2, 3 in both normal and injured ACL fibroblasts. The decrease of LOXs and increase of MMP-1, 2, 3 in ACLfs response to TNF-α were further promoted by coculture. Taken together, these results show for the first time that the crosstalk between ACLfs and SCs could modulate the LOXs and MMP-1, 2, 3 synthesis in ACLfs in the presence of TNF-α. Accumulation of MMPs in the isolated fluid-containing space not only disrupts the balance of ACL healing, but also increases cartilage degradation and accelerates osteoarthritis (OA) in injured joint. Based on this mechanism, targeting inhibition of MMPs could provide a promising therapeutic strategy for acute ligament injury.

Keywords

ACL - synoviocytes - MMPs - LOXs - coculture

Volltext

Referenzen

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