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Thromb Haemost 1994; 71(01): 026-031
DOI: 10.1055/s-0038-1642380
Review Article
Schattauer GmbH Stuttgart

Platelet Aggregation and In Vivo Shear Forces

Madeleine M Mazeaud
1   Pharmacology, CNRS URA 1482, Faculté de Médecine Necker, INSERM U 28, Hôpital Broussais, Paris, France
,
Jaime Levenson
2   Centre de Médecine Préventive Cardiovasculaire, INSERM U 28, Hôpital Broussais, Paris, France
,
Kim Hanh Le Quan Sang
1   Pharmacology, CNRS URA 1482, Faculté de Médecine Necker, INSERM U 28, Hôpital Broussais, Paris, France
,
Alain Simon
2   Centre de Médecine Préventive Cardiovasculaire, INSERM U 28, Hôpital Broussais, Paris, France
,
Marie-Aude Devynck
1   Pharmacology, CNRS URA 1482, Faculté de Médecine Necker, INSERM U 28, Hôpital Broussais, Paris, France
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Publikationsverlauf

Received: 28. Juni 1993

Accepted after revision 30. August 1993

Publikationsdatum:
12. Juli 2018 (online)

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Summary

Haemodynamic shear forces have been reported to exert direct and indirect effects on platelet reactivity. In vitro, they activate platelets leading to spontaneous or facilitated aggregation. In vivo, they stimulate the production of endothelium-derived antiaggregatory agents. This study was designed to evaluate in hypertensive patients, before and after antihypertensive treatment, the possible role of these haemodynamic forces, determined at the brachial artery level on the ex vivo platelet aggregatory response to ADP and collagen. Platelet reactivity, evaluated by EC50 for ADP and collagen, was found to be related to blood velocity, shear rate and shear stress (p <0.01 for each). These inverse correlations of platelet aggregation with stress levels did not depend on age, body mass index, mean blood pressure, serum cholesterol and triglycerides or haematocrit. They were also independent of platelet cytosolic Ca2+ and cyclic AMP.

The changes in shear forces and in aggregatory responses to ADP and collagen induced by nitrendipine treatment for 6 months remained negatively correlated, confirming the relationships existing between haemodynamic shear forces and platelet reactivity.

These results indicate that the shear antiaggregant effects, likely mediated by tlow-dependent endothelium-derived factors, prevail over its direct platelet aggregating effects.

 

  • References

  • 1 Gear AR L. Rapid platelet morphological changes visualized by scanning-electron microscopy: kinetics derived from a quenched-flow approach. British J Haematol 1984; 56: 387-98
    CrossrefPubMedSuche in Google Scholar
  • 2 O’Brien JR. Shear-induced platelet aggregation. Lancet 1990; 335: 711-3
    CrossrefPubMedSuche in Google Scholar
  • 3 Yung W, Frojmovic MM. Platelet aggregation in laminar flow. I. Adenosine diphosphate concentration, time and shear rate dependence. Thromb Res 1982; 28: 361-77
    CrossrefPubMedSuche in Google Scholar
  • 4 Bell DN, Spain S, Goldsmith HL. Adenosine diphosphate-induced aggregation of human platelets in flow through tubes. II. Effect of shear rate, donor sex and ADP concentration. Biophys J 1989; 56: 829-43
    CrossrefPubMedSuche in Google Scholar
  • 5 Ikeda Y, Handa M, Kawano K, Kamata T, Murata M, Araki Y, Anbo H, Kawai Y, Watanabe K, Itagaki I, Sakai K, Ruggieri ZM. The role of von Willebrand factor and fibrinogen in platelet aggregation under varying stress. J Clin Invest 1991; 87: 1234-40
    CrossrefPubMedSuche in Google Scholar
  • 6 Baumgartner HR, Turitto V, Weiss HJ. Effect of shear rate on platelet interaction with subendothelium in citrated and native blood. J Lab Clin Med 1980; 95: 208-21
    PubMedSuche in Google Scholar
  • 7 Olson JD, Zaleski A, Herrmann D, Flood PA. Adhesion of platelets to purified solid-phase von Willebrand faetoi. Effccb uf wall slieax rate, ADP, thrombin and ristocetin. J Lab Clin Med 1989; 114: 6-18
    PubMedSuche in Google Scholar
  • 8 Giorgio TD, Heliums JD. A cone and plate viscometer for the continuous measurement of blood platelet activation. Biorheology 1988; 25: 605-24
    CrossrefPubMedSuche in Google Scholar
  • 9 Kawakami K, Fukuyama M, Sakai K, Itagaki I, Kawano K, Handa M, Ikeda Y. Change in intracellular calcium during shear induced platelet aggregation. ASAIO Trans 1990; 36: M696-9
    PubMedSuche in Google Scholar
  • 10 Moritz MW, Reimers RC, Baker RK, Sutera SP, Joist JH. Role of cytoplasmic and releasable ADP in platelet aggregation induced by laminar shear stress. J Lab Clin Med 1983; 101: 537-44
    PubMedSuche in Google Scholar
  • 11 Wurzinger LJ, Opitz R, Blassberg P, Schmid-Schonbein H. Platelet and coagulaton parameters following millisecond exposure to laminar shear stress. Thromb Haemost 1985; 54: 381-6
    Thieme ConnectPubMedSuche in Google Scholar
  • 12 Kroll MH, Heliums JD, Guo Z, Durante W, Razdan K, Hrbolich JK, Schafer AI. Protein kinase C is activated in platelets subjected to pathological shear stress. J Biol Chem 1993; 268: 3520-4
    PubMedSuche in Google Scholar
  • 13 Schwartz CJ, Sprague EA. Vascular endothelium and hemodynamic stress. Nutr Metab Cardiovasc Dis 1992; 2: 99-100
    PubMedSuche in Google Scholar
  • 14 Larsen FL, Katz S, Roufogalis BD, Brookst DE. Physiological shear stresses enhance the Ca2+ permeability of human erythrocytes. Nature 1981; 294: 667-8
    CrossrefPubMedSuche in Google Scholar
  • 15 Frangos JA, Eskin SG, Mclntire LV. Flow effects on prostacyclin production by cultured human endothelial cells. Science 1985; 227: 1477-9
    CrossrefPubMedSuche in Google Scholar
  • 16 Grabovski EF, Jaffe EA, Weksler BB. Prostacyclin production by cultured endothelium cell monolayers exposed to step increases in shear stress. J Lab Clin Med 1985; 105: 36-43
    PubMedSuche in Google Scholar
  • 17 Rubanyi GM, Vanhoutte PM. Oxygen-derived free radicals, endothelium and responsiveness of vascular smooth muscle. Am J Physiol 1986; 250: H822-7
    PubMedSuche in Google Scholar
  • 18 Yoshizumi M, Kurihara H, Sugiyama T, Takaku F, Yanagisawa M, Masaki T, qYazaki Y. Hemodynamic shear stress stimulates endothelin production by cultured endothelium cells. Biochem Biophys Res Commun 1989; 161: 859-64
    CrossrefPubMedSuche in Google Scholar
  • 19 Milner P, Bodin P, Loesch A, Burnstock G. Rapid release of endothelin and ATP from isolated aortic endothelial cells exposed to increased flow. Biochem Biophys Res Commun 1990; 649-56
    PubMedSuche in Google Scholar
  • 20 Nollert MU, Diamond SK, Mc Intyre LV. Hydrodynamic shear stress and mass transport modulation of endothelial cell metabolism. Biotechnol Bioeng 1991; 38: 588-602
    CrossrefPubMedSuche in Google Scholar
  • 21 Radomski MW, Palmer RM J, Moncada S. The anti-aggregating properties of vascular endothelium: interactions between prostacyclin and nitric oxide. Br J Pharmacol 1987; 92: 639-46
    CrossrefPubMedSuche in Google Scholar
  • 22 Astarie-Dequeker C, Iouzalen L, David-Dufilho M, Devynck M-A. In vitro inhibition by endothelins of thrombin-induced aggregation and Ca2+ mobilization in human platelets. Br J Pharmacol 1992; 106: 966-71
    CrossrefPubMedSuche in Google Scholar
  • 23 Diamond SL, Eskin SG, Mclntire LV. Fluid flow stimulates tissue plasminogen activator secretion by cultured human endothelium cells. Science 1989; 243: 1483-5
    CrossrefPubMedSuche in Google Scholar
  • 24 Simon A, Levenson J. Abnormal wall shear conditions in the brachial artery of hypertensive patients. J Hypertens 1990; 8: 109-14
    CrossrefPubMedSuche in Google Scholar
  • 25 Badimon JJ, Badimon L, Turitto VT, Fuster V. Platelet deposition at high shear rates is enhanced by high plasma cholesterol levels. Arterioscler Thromb 1991; 11: 395-402
    CrossrefPubMedSuche in Google Scholar
  • 26 Carvalho AC A, Colman RW, Lees RS. Platelet function in hyperlipoproteinemia. N Engl J Med 1974; 290: 434-8
    CrossrefPubMedSuche in Google Scholar
  • 27 Tremoli E, Maderna P, Colli S, Morazzoni G, Sirtori M, Sirtori CR. Increased platelet sensitivity and thromboxane B2 formation in type-II hyperlipoproteinaemic patients. Eur J Clin Invest 1984; 14: 329-33
    CrossrefPubMedSuche in Google Scholar
  • 28 Ellefson RD, Caraway WT. Blood lipids. In: Fundamentals of clinical chemistry. Trietz NM. (ed.) WB Saunders Co; Philadelphia: 1982: 509-10
    Suche in Google Scholar
  • 29 Simon A, Levenson J, Cambien F, Bouthier J. Combined effects of gender and hypertension on the geometric design of large arteries. Sexual differences in normal and hypertensive forearm arteries. Am J Hypertens 1988; 1: 119-23
    CrossrefPubMedSuche in Google Scholar
  • 30 Simon A, Flaud P, Levenson J. Pulsatile flow and oscillating wall shear stress in the brachial artery of normotensive and hypertensive subjects. Cardiovasc Res 1990; 2: 129-36
    PubMedSuche in Google Scholar
  • 31 Levenson J, Simon A, Cambien F, Beretti C. Cigarette smoking and hypertension: factors independently associated with blood hyperviscosity and arterial rigidity. Arteriosclerosis 1987; 2: 572-7
    PubMedSuche in Google Scholar
  • 32 Levenson J, Simon A, Pithois-Merli I. Brachial arterial changes in wrist exclusion in normotensive and hypertensive men. Am J Physiol 1987; 253: H217-H224
    PubMedSuche in Google Scholar
  • 33 Born GV R. Aggregation of blood platelets by adenosine and its reversal. Nature 1962; 195: 927-9
    CrossrefPubMedSuche in Google Scholar
  • 34 Le Quan Sang KH, Devynck MA. Increased platelet cytosolic free calcium concentration in essential hypertension. J Hypertension 1986; 4: 567-74
    CrossrefPubMedSuche in Google Scholar
  • 35 Mazeaud MM, Le Quan Sang KH, Devynck MA. Platelet cyclic AMP in essential hypertension. J Hypertens 1989; 7: 501-6
    CrossrefPubMedSuche in Google Scholar
  • 36 Moncada S, Gryglewski RT, Vane JR. An enzyme isolated from arteries transforms prostaglandin endoperoxydes to an unstable substance that inhibits platelet aggregation. Nature 1976; 263: 663-5
    CrossrefPubMedSuche in Google Scholar
  • 37 Azuma H, Ishikawa M, Sekisaki S. Endothelium-dependent inhibition of platelet aggregation. Br J Pharmacol 1986; 88: 411-5
    CrossrefPubMedSuche in Google Scholar
  • 38 Johnson M, Ramey E, Ramwell PW. Sex and age differences in human platelet aggregation. Nature 1975; 253: 355-7
    CrossrefPubMedSuche in Google Scholar
  • 39 Mazeaud MM, Driss F, Le Quan Sang KH, Duranthon V, Levenson J, Simon A, Devynck MA. Biochemical and functional alterations associated with hypercholesterolemia in platelets from hypertensive patients. Atherosclerosis 1992; 94: 201-11
    CrossrefPubMedSuche in Google Scholar
  • 40 Rink TJ, Smith SW, Tsien RY. Cytosolic free Ca2+ in human platelets: Ca2+ threshold and Ca2+-independent activation for shape-change and secretion. FEBS Lett 1982; 148: 21-6
    CrossrefPubMedSuche in Google Scholar
  • 41 Siess W. Molecular mechanisms of platelet activation. Physiol Rev 1989; 69: 58-178
    CrossrefPubMedSuche in Google Scholar
  • 42 Nyrop M, Zweifler AJ. Platelet aggregation in hypertension and the effects of antihypertensive treatment. J Hypertens 1988; 6: 263-9
    PubMedSuche in Google Scholar
  • 43 Blache D, Ojeda C. Comparative inhibitory effects of dihydropyridines on platelet aggregation, calcium uptake and cyclic AMP concentration. Pharmacology 1992; 45: 250-9
    CrossrefPubMedSuche in Google Scholar
  • 44 Godfraind T, Miller R, Wibo M. Calcium antagonism and calcium entry blockade. Pharmacol Rev 1986; 38: 321-416
    PubMedSuche in Google Scholar
  • 45 Zschauer A, Van Breemer C, Biihler FR, Nelson MT. Calcium channels in thrombin-activated human platelet membrane. Nature 1988; 334: 703-5
    CrossrefPubMedSuche in Google Scholar
  • 46 Vanhoutte PM. Role of calcium and endothelium in hypertension, cardiovascular disease, and subsequent vascular events. J Cardiovasc Pharmacol 1992; 19 Suppl. (Suppl. 03) S6-S10
    CrossrefPubMedSuche in Google Scholar