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Thromb Haemost 1994; 71(01): 026-031
DOI: 10.1055/s-0038-1642380
Review Article
Schattauer GmbH Stuttgart

Platelet Aggregation and In Vivo Shear Forces

Madeleine M Mazeaud
1   Pharmacology, CNRS URA 1482, Faculté de Médecine Necker, INSERM U 28, Hôpital Broussais, Paris, France
,
Jaime Levenson
2   Centre de Médecine Préventive Cardiovasculaire, INSERM U 28, Hôpital Broussais, Paris, France
,
Kim Hanh Le Quan Sang
1   Pharmacology, CNRS URA 1482, Faculté de Médecine Necker, INSERM U 28, Hôpital Broussais, Paris, France
,
Alain Simon
2   Centre de Médecine Préventive Cardiovasculaire, INSERM U 28, Hôpital Broussais, Paris, France
,
Marie-Aude Devynck
1   Pharmacology, CNRS URA 1482, Faculté de Médecine Necker, INSERM U 28, Hôpital Broussais, Paris, France
› Author Affiliations
Further Information

Publication History

Received: 28 June 1993

Accepted after revision 30 August 1993

Publication Date:
12 July 2018 (online)

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Summary

Haemodynamic shear forces have been reported to exert direct and indirect effects on platelet reactivity. In vitro, they activate platelets leading to spontaneous or facilitated aggregation. In vivo, they stimulate the production of endothelium-derived antiaggregatory agents. This study was designed to evaluate in hypertensive patients, before and after antihypertensive treatment, the possible role of these haemodynamic forces, determined at the brachial artery level on the ex vivo platelet aggregatory response to ADP and collagen. Platelet reactivity, evaluated by EC50 for ADP and collagen, was found to be related to blood velocity, shear rate and shear stress (p <0.01 for each). These inverse correlations of platelet aggregation with stress levels did not depend on age, body mass index, mean blood pressure, serum cholesterol and triglycerides or haematocrit. They were also independent of platelet cytosolic Ca2+ and cyclic AMP.

The changes in shear forces and in aggregatory responses to ADP and collagen induced by nitrendipine treatment for 6 months remained negatively correlated, confirming the relationships existing between haemodynamic shear forces and platelet reactivity.

These results indicate that the shear antiaggregant effects, likely mediated by tlow-dependent endothelium-derived factors, prevail over its direct platelet aggregating effects.

 

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