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Thromb Haemost 1994; 71(01): 054-061
DOI: 10.1055/s-0038-1642384
Review Article
Schattauer GmbH Stuttgart

Recombinant Rat Protein C: Comparative Studies of Structure, Function and Synthesis with Plasma Protein C

Mayumi Ono
The Molecular Biology Research Laboratory, Daiichi Pharmaceutical Co., Ltd., Tokyo 134, Japan
,
Hiroyuki Fujiwara
The Molecular Biology Research Laboratory, Daiichi Pharmaceutical Co., Ltd., Tokyo 134, Japan
,
Takaaki Okafuji
The Molecular Biology Research Laboratory, Daiichi Pharmaceutical Co., Ltd., Tokyo 134, Japan
,
Tomoko Enjoh
The Molecular Biology Research Laboratory, Daiichi Pharmaceutical Co., Ltd., Tokyo 134, Japan
,
Katsuhiko Nawa
The Molecular Biology Research Laboratory, Daiichi Pharmaceutical Co., Ltd., Tokyo 134, Japan
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Further Information

Publication History

Received: 09 June 1993

Accepted after revision 21 September 1993

Publication Date:
12 July 2018 (online)

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Summary

In order to elucidate the role of protein C (PC) in the rat, we expressed, purified, and characterized recombinant rat PC. The purified recombinant rat PC was 70–90% two-chain (41 kDa heavy chain; 22 and 23 kDa light chain) and 10–30% single-chain (61 kDa). Amino acid analysis confirmed the presence of 10 moles of γ-carboxyglutamic acid residues per mol of protein. For comparison, plasma rat PC was purified from a barium citrate precipitate using similar method. Plasma rat PC was a two-chain form (41 kDa heavy chain; 22 kDa light chain) with no detectable single-chain nor 23 kDa light chain. For determination of the in vitro secreted species, primary cultured rat hepatocytes were incubated for 6 h with methionine-free MEM containing vitamin K1, aprotinin, and [35S]methionine. The supernatant was immunoprecipitated and analyzed by SDS-PAGE followed by autoradiography. Approximately 90% of the PC radioactivity migrated as a two-chain molecule. These results indicate that rat PC is secreted mainly as a two-chain molecule from the liver. PROTAC-activated forms of recombinant rat PC, plasma rat PC, and plasma human PC hydrolyzed the S-2366 chromogenic substrate at the same rate Recombinant rat PC was also activated by the thrombin-thrombomodulin complex at a rate similar to plasma lat PC. The anticoagulant activities of the three activated PCs were examined in rat plasma. Both recombinant and plasma rat PC prolonged the activated partial thromboplastin time in a dose-dependent manner, but plasma human PC was less effective. These results suggest that recombinant rat PC is applicable for in vivo thrombosis studies in the rat.

 

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