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Thromb Haemost 1994; 71(05): 670-674
DOI: 10.1055/s-0038-1642501
Review Article
Schattauer GmbH Stuttgart

Effect of Human IgG Antiphospholipid Antibodies on an In Vivo Thrombosis Model in Mice

Silvia S Pierangeli
1   The Division of Rheumatology, Department of Medicine, University of Louisville, Louisville, KY, USA
,
John H Barker
2   The Division of Plastic Surgery, Department of Surgery, University of Louisville, Louisville, KY, USA
,
Dejzi Stikovac
1   The Division of Rheumatology, Department of Medicine, University of Louisville, Louisville, KY, USA
,
Douglas Ackerman
3   The Department of Pathology, Alliant Health System, Norton and Kosair’s Children Hospital, Louisville, KY, USA
,
Gary Anderson
4   The Department of Physiology, University of Louisville, Louisville, KY, USA
,
Jordi Barquinero
1   The Division of Rheumatology, Department of Medicine, University of Louisville, Louisville, KY, USA
,
Robert Acland
2   The Division of Plastic Surgery, Department of Surgery, University of Louisville, Louisville, KY, USA
,
E Nigel Harris
1   The Division of Rheumatology, Department of Medicine, University of Louisville, Louisville, KY, USA
› Author Affiliations
Further Information

Publication History

Received 17 August 1993

Accepted after resubmission 25 January 1994

Publication Date:
06 July 2018 (online)

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Summary

High levels of IgG antiphospholipid antibodies (aPL) have been associated with clinical thrombosis. It is uncertain however whether these antibodies play a direct role in thrombosis or are merely epiphenomena. To investigate whether antiphospholipid antibodies might play a role in thrombosis, we utilized a novel mouse model in which the dynamics of in vivo thrombosis can be studied. CD1 mice (26-30 g) were passively immunized with 25 mg of human IgG from a patient with the Antiphospholipid Syndrome (IgG-APS) (n = 17), IgG from normal pooled sera (TgG-NHS) (n = 9). or saline solution (n = 17), followed by 40 mg of the same preparations at 48 h. At 72 h, levels of human aPL antibodies, detected using the anticardiolipin ELISA test (aCL ELISA test), in mice immunized with IgG-APS, were 50-100 GPL units. Each animal was anesthetized, femoral vein minimally mobilized and subjected to a standardized “pinch” injury to induce thrombosis. The vessel was transilluminated using acrylic optical fibers connected to a light source, and clot formation and dissolution were visualized by a standard surgical microscope equipped with a video camera, video recorder, and computer assisted analysis system. Results showed that average clot size was significantly larger in mice immunized with IgG-APS compared to those treated with saline (p <0.037). In addition, the thrombus persisted longer in a significantly higher number of mice immunized with IgG-APS (10/17) compared to mice immunized with IgG-NHS (1/9) or saline (2/12) (p <0.02). These data suggest that IgG-APS may play a role in thrombus formation in humans. In addition, this study shows the feasibility of using this in vivo murine thrombus model for study of the effects of aPL antibodies.

 

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