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Thromb Haemost 1988; 59(01): 023-028
DOI: 10.1055/s-0038-1642559
Review Article
Schattauer GmbH Stuttgart

Thromboembolic Reaction Following Wall Puncture in Arterioles and Venules of the Rabbit Mesentery

Mirjam G A oude Egbrink
The Departments of Physiology and Biophysics, Laboratory for Microcirculation, Biomedical Center, University of Limburg, Maastricht, The Netherlands
,
Geert Jan Tangelder
The Departments of Physiology and Biophysics, Laboratory for Microcirculation, Biomedical Center, University of Limburg, Maastricht, The Netherlands
,
Dick W Slaaf
The Departments of Physiology and Biophysics, Laboratory for Microcirculation, Biomedical Center, University of Limburg, Maastricht, The Netherlands
,
Robert S Reneman
The Departments of Physiology and Biophysics, Laboratory for Microcirculation, Biomedical Center, University of Limburg, Maastricht, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 10 March 1987

Accepted after revision 23 September 1987

Publication Date:
18 April 2018 (online)

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Summary

The walls of rabbit mesenteric arterioles and venules (diameter 20 to 40 pm) were punctured with glass micropipets (tip diameter 6 to 8 pm). Thromboembolic reactions resulting from this standardized, small mechanical vessel wall injury could be quantified in vivo with the use of intravital video-microscopy. Following induction of the injury thrombus growth started immediately (<0.1 s). Bleeding times were short, on the average less than 2 s, and did not differ between arterioles and venules. The duration of the embolization process was significantly longer in arterioles than in venules (median 101 and 17 s, respectively), and more emboli were produced in arterioles than in venules (median 6 and 1, respectively). Arteriolar thrombi were more effective in plugging the punctured holes than venular thrombi. The differences in thromboembolic reaction between arterioles and venules, as found in the present study, can probably not be explained by fluid dynamic factors.

Key words

Vessel wall injury - Thrombus formation - Blood platelets - Intravital microscopy - Fluid dynamics
 

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