PDF herunterladen
Thromb Haemost 1988; 59(01): 023-028
DOI: 10.1055/s-0038-1642559
Review Article
Schattauer GmbH Stuttgart

Thromboembolic Reaction Following Wall Puncture in Arterioles and Venules of the Rabbit Mesentery

Mirjam G A oude Egbrink
The Departments of Physiology and Biophysics, Laboratory for Microcirculation, Biomedical Center, University of Limburg, Maastricht, The Netherlands
,
Geert Jan Tangelder
The Departments of Physiology and Biophysics, Laboratory for Microcirculation, Biomedical Center, University of Limburg, Maastricht, The Netherlands
,
Dick W Slaaf
The Departments of Physiology and Biophysics, Laboratory for Microcirculation, Biomedical Center, University of Limburg, Maastricht, The Netherlands
,
Robert S Reneman
The Departments of Physiology and Biophysics, Laboratory for Microcirculation, Biomedical Center, University of Limburg, Maastricht, The Netherlands
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 10. März 1987

Accepted after revision 23. September 1987

Publikationsdatum:
18. April 2018 (online)

Auch verfügbar auf
  als PDF herunterladen  Lizenzen und Reprints

Summary

The walls of rabbit mesenteric arterioles and venules (diameter 20 to 40 pm) were punctured with glass micropipets (tip diameter 6 to 8 pm). Thromboembolic reactions resulting from this standardized, small mechanical vessel wall injury could be quantified in vivo with the use of intravital video-microscopy. Following induction of the injury thrombus growth started immediately (<0.1 s). Bleeding times were short, on the average less than 2 s, and did not differ between arterioles and venules. The duration of the embolization process was significantly longer in arterioles than in venules (median 101 and 17 s, respectively), and more emboli were produced in arterioles than in venules (median 6 and 1, respectively). Arteriolar thrombi were more effective in plugging the punctured holes than venular thrombi. The differences in thromboembolic reaction between arterioles and venules, as found in the present study, can probably not be explained by fluid dynamic factors.

Key words

Vessel wall injury - Thrombus formation - Blood platelets - Intravital microscopy - Fluid dynamics
 

  • References

  • 1 Arfors K-E, Dhall DP, Engeset I, Hint H, Matheson NA, Tangen O. Biolaser endothelial trauma as a means of quantifying platelet activity in vivo. Nature 1968; 218: 887-888
    CrossrefPubMedGoogle Scholar
  • 2 Mc Kenzie FN, Arfors K-E, Matheson NA. Measurement of the platelet response to laser-induced microvascular injury. Assessment of determinants of platelet aggregation in vivo. Thromb Diathes Haemorrh 1974; 32: 704-713
    PubMedGoogle Scholar
  • 3 Wiedeman MP. Vascular reactions to laser in vivo. Microvasc Res 1974; 8: 132-138
    CrossrefPubMedGoogle Scholar
  • 4 Hovig T, Mc Kenzie FN, Arfors K-E. Measurement of the platelet response to laser-induced microvascular injury. Ultrastructural studies. Thromb Diathes Haemorrh 1974; 32: 695-703
    PubMedGoogle Scholar
  • 5 Herrmann KS. Platelet aggregation induced in the hamster cheek pouch by a photochemical process with excited fluorescein isothiocyanate-dextran. Microvasc Res 1983; 26: 238-249
    CrossrefPubMedGoogle Scholar
  • 6 Sato M, Ohshima N. Platelet thrombus induced in vivo by filtered light and fluorescent dye in mesenteric microvessels of the rat. Thromb Res 1984; 35: 319-334
    CrossrefPubMedGoogle Scholar
  • 7 Herrmann KS, Voigt W-H. Ultrastructural observations of an electron dense amorphous layer on selectively damaged endothelial cells, a possible trigger of thrombogenesis in vivo, and its inhibition by nafazatrom. Thromb Res 1984; 36: 205-215
    CrossrefPubMedGoogle Scholar
  • 8 Apitz K. Die Bedeutung der Gerinnung und Thrombose für die Blutstillung. Virchows Arch [A] 1942; 308: 540-614
    CrossrefPubMedGoogle Scholar
  • 9 Zucker MB. Platelet agglutination and vasoconstriction as factors in spontaneous hemostasis in normal, thrombocytopenic, heparinized and hypoprothrombinémie rats. Am J Physiol 1947; 148: 275-288
    PubMedGoogle Scholar
  • 10 Hugues J. Contribution à l’étude des facteurs vasculaires et sanguins dans l’hémostase spontanée. Arch Int Physiol 1953; 61: 565-711
    PubMedGoogle Scholar
  • 11 Bergqvist D. Haemostatic plug formation in the rabbit mesentery. A methodological study. Ups J Med Sci 1974; 79: 28-38
    CrossrefPubMedGoogle Scholar
  • 12 Chen TI, Tsai C. The mechanism of haemostasis in peripheral vessels. J Physiol 1948; 107: 280-288
    CrossrefPubMedGoogle Scholar
  • 13 Herrmann RG, Frank JD, Marlett DL. An in vivo technique for assessing the formation of a hemostatic platelet plug. Proc Soc Exp Biol Med 1968; 128: 960-964
    CrossrefPubMedGoogle Scholar
  • 14 Tangelder GJ, Slaaf DW, Reneman RS. Fluorescent labeling of blood platelets in vivo. Thromb Res 1982; 28: 803-820
    CrossrefPubMedGoogle Scholar
  • 15 Tangelder GJ, Slaaf DW, Teirlinck HC, Alewijnse R, Reneman RS. Localization within a thin optical section of fluorescent blood platelets flowing in a microvessel. Microvasc Res 1982; 23: 214-230
    CrossrefPubMedGoogle Scholar
  • 16 Tangelder GJ, Teirlinck HC, Slaaf DW, Reneman RS. Distribution of blood platelets flowing in arterioles. Am J Physiol 1985; 248: H318-H323
    PubMedGoogle Scholar
  • 17 Tangelder GJ, Slaaf DW, Muijtjens AM M, Arts T, oude Egbrink MG A, Reneman RS. Velocity profiles of blood platelets and red blood cells flowing in arterioles of the rabbit mesentery. Circ Res 1986; 59: 505-514
    CrossrefPubMedGoogle Scholar
  • 18 Slaaf DW, Alewijnse R, Wayland H. Use of telescopic imaging in intravital microscopy: a simple solution for conventional microscopes. Int J Microcirc Clin Exp 1982; 1: 121-134
    PubMedGoogle Scholar
  • 19 Siegenbeek van Heukelom J, Dijkstra K, van Ingcn H. A versatile and reproducibly operating microelectrode puller. Med Biol Eng 1976; 3: 644-652
    PubMedGoogle Scholar
  • 20 Intaglietta M, Tompkins WR. Microvascular measurements by video image shearing and splitting. Microvasc Res 1973; 5: 309-312
    CrossrefPubMedGoogle Scholar
  • 21 Slaaf DW, Rood JP S M, Tangelder GJ, Jeurens TJ M, Alewijnse R, Reneman RS, Arts T. A bidirectional optical (BDO) three-stage prism grating system for on-line measurement of red blood cell velocity in microvessels. Microvasc Res 1981; 22: 110-122
    CrossrefPubMedGoogle Scholar
  • 22 Slaaf DW, Tangelder GJ, Reneman RS, Arts T. Dual sensor model calculations applied to blood cell velocity profiles obtained by direct observation in vivo. Int J Microcirc Clin Exp 1986; 5: 2-12
    PubMedGoogle Scholar
  • 23 Burns KF, de Lannoy CW. Compendium of normal blood values of laboratory animals with indication of variations. I. Random-sexed populations of small animals. Toxicol Appl Pharmacol 1966; 8: 429-437
    CrossrefPubMedGoogle Scholar
  • 24 Kozma C, Mncltlin W, Cummins LM, Manor B. The anatomy, physiology, and the biochemistry of the rabbit. In: The biology of the laboratory rabbit. Weisbroth SH, Flatt RE, Kraus AL. (eds) Academic Press; New York: 1974: 50-72
    Google Scholar
  • 25 Arfors K-E, Arturson G, Bergqvist D, Svensjoe E. The effect of inhibition of prostaglandin synthesis on microvascular haemostasis and macromolecular leakage. Thromb Res 1976; 8: 393-402
    PubMedGoogle Scholar
  • 26 Schmid-Schoenbein H, Rieger H, Fischer Th. Fluid-dynamic boundary conditions for thrombotic processes in high shear environments in vivo. In: Blood vessels: problems arising at the border of natural and artificial blood vessels. Effert S, Meyer-Erkelenz JD. (eds) Springer; Berlin: 1976: 57-63
    Google Scholar
  • 27 Born GV R. Observations on the change in shape of blood platelets brought about by adenosine diphosphate. J Physiol 1970; 209: 487-511
    CrossrefPubMedGoogle Scholar
  • 28 Arfors K-E, Cockburn JS, Gross JF. Measurement of growth rate of laser-induced intravascular platelet aggregation and the influence of blood flow velocity. Microvasc Res 1976; 11: 79-87
    CrossrefPubMedGoogle Scholar
  • 29 Rogers AB. The effect of pH on human platelet aggregation induced by epinephrine and ADP. Proc Soc Exp Biol Med 1972; 139: 1100-1103
    CrossrefPubMedGoogle Scholar
  • 30 Tang SS, Frojmovic MM. The effects of pCO2 and pH on platelet shape change and aggregation for human and rabbit platelet-rich plasma. Thromb Res 1977; 10: 135-145
    CrossrefPubMedGoogle Scholar
  • 31 Coccheri S, Astrup T. Thromboplastic and fibrinolytic activities of large human vessels. Proc Soc Exp Biol Med 1961; 108: 369-372
    CrossrefPubMedGoogle Scholar
  • 32 Noordhoek Hegt V. Relations between activation and inhibition of fibrinolysis in the walls of human arteries and veins. Thromb Haemostas 1977; 38: 407-419
    PubMedGoogle Scholar
  • 33 Mehta J, Roberts A. Human vascular tissues produce thromboxane as well as prostacyclin. Am J Physiol 1983; 244: R839-R844
    PubMedGoogle Scholar