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Thromb Haemost 1988; 59(01): 040-044
DOI: 10.1055/s-0038-1642562
Review Article
Schattauer GmbH Stuttgart

Characterisation and Some Properties of the Protein C Activator from Agkistrodon Contortrix Contortrix Venom

Thomas Exner
The Haematology Department, Westmead Hospital, Westmead, Australia
,
Riita Vaasjoki
The Haematology Department, Westmead Hospital, Westmead, Australia
› Author Affiliations
Further Information

Publication History

Received 01 October 1986

Accepted after revision 28 September 1987

Publication Date:
18 April 2018 (online)

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Summary

The protein C activator (PCA) detectable in the venom of Agkistrodon Contortrix Contortrix (ACCV, Southern Copperhead) by specific immunochromometric assay and anticoagulant activity has been isolated and partially characterized. Chromatog raphy of the crude venom on SP-Sephadex followed by Con A Sepharose and finally on hydroxylapatite was necessary to achieve an electrophoretically – pure product. The isolated PCA is a single chain glycoprotein with strong positive charge and an apparent molecular weight of 36,000.

It had an immediate-inhibiting effect on the activated partial thromboplastin time (APTT) of normal plasma with no noticeable eilect Oii the piOtliiumbm time, its piolonging eifect oil the APTT was dependent on protein C and il appeared to interfere with the contact mechanism rather than with factors V and VIII. It had enzymatic activity on some tripeptide chromogenic substrates sensitive to thrombin and kallikrein. When mixed with normal plasma it generated activity on substrates sensitive to activated protein C and should be useful for studies of protein C.

Key words

Agkistrodon Contortrix Contortrix - Venom protein C activator - Anticoagulant - Chromatography - Immunochromometric assay - Chromogenic substrates
 

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