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Thromb Haemost 1988; 59(01): 077-079
DOI: 10.1055/s-0038-1642569
Review Article
Schattauer GmbH Stuttgart

A Plasminogen Activator Inhibitor (PAI-2) Circulates in Two Molecular Forms During Pregnancy

Nuala A Booth
The Department of Medicine and Therapeutics, University of Aberdeen, UK
,
Alison Reith
The Department of Medicine and Therapeutics, University of Aberdeen, UK
,
Bruce Bennett
The Department of Medicine and Therapeutics, University of Aberdeen, UK
› Author Affiliations
Further Information

Publication History

Received 07 July 1987

Accepted after revision 09 October 1987

Publication Date:
18 April 2018 (online)

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Summary

During normal pregnancy maternal haemostasis alters to protect against bleeding with a rise in plasma clotting factor levels and increased inhibition of fibrinolysis. The latter is due in part to increased levels of the 48 kDa plasminogen activator inhibitor (PAI-1) present in the plasma of non-pregnant individuals. A second plasminogen activator inhibitor (PAI-2) occurs in placenta and in a cultured histiocytic lymphoma cell line. We report here the identification by SDS-PAGE and zymography of PAI-2 in plasma during normal pregnancy. PAI-2 was present in two molecular forms of about 75 and 130 kDa, which were detectable at 12 weeks gestation and which persisted in the maternal circulation for up to 7 days after delivery. These forms of PAI-2 appear to be distinct from purifed PAI-2, which has a molecular mass of 47–60 kDa and which is not normally detectable in this zymographic system, since it is sensitive to denaturants. The novel forms of PAI-2 may represent complexes or aggregates that retain activity after SDS-PAGE.

Keywords

Plasminogen activator inhibitor - Pregnancy - Inhibition of fibrinolysis
 

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