Download PDF
Thromb Haemost 1988; 59(02): 143-146
DOI: 10.1055/s-0038-1642743
Original Articles
Schattauer GmbH Stuttgart

Effect of 4-Hydroxy-2,3-trans-nonenal on Platelet Function

Michael L Selley
The Cardiovascular Research Group, Department of Medicine and Clinical Science, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
,
Julie A McGuiness
The Cardiovascular Research Group, Department of Medicine and Clinical Science, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
,
Lynn A Jenkin
The Cardiovascular Research Group, Department of Medicine and Clinical Science, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
,
Mark R Bartlett
The Cardiovascular Research Group, Department of Medicine and Clinical Science, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
,
Neville G Ardlie
The Cardiovascular Research Group, Department of Medicine and Clinical Science, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
› Author Affiliations
Further Information

Publication History

Received 16 June 1987

Accepted after revision 29 October 1987

Publication Date:
21 May 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

4-Hydroxy-2,3-trans-nonenal (HNE), an aldehyde end-product of lipid peroxidation, potentiated aggregation and increased thromboxane A2 formation in platelets challenged with ADP, thrombin or the ionophore A23187. These effects were observed at HNE concentrations in the range 10-100 μM. Platelet responses to collagen, epinephrine and arachidonic acid were not affected by PINE. Concentrations of HNE in excess of 100 pM inhibited platelet activation. HNE increased the release of 3H-arachidonic acid from prelabelled platelet phospholipids in response to thrombin or ADR It is proposed that HNE may play an important role in controlling platelet function by regulating the activity of phospholipase A2.

Keywords

Platelet - 4-Hydroxy-2,3-trans-nonenal-lipid peroxidation
 

  • References

  • 1 Zucker MB, Nachmias VT. Platelet activation. Atherosclerosis 1985; 5: 2-18
    PubMedGoogle Scholar
  • 2 Ross R. Atherosclerosis: a problem of the biology of arterial cell walls and their interactions with blood components. Atherosclerosis 1981; 1: 293-311
    PubMedGoogle Scholar
  • 3 Hamberg M, Svensson J, Samuelsson B. Thromboxanes: a new group of biologically active compounds derived from prostaglandin endoperoxides. Proc Natl Acad Sci USA 1975; 72: 2994-2998
    CrossrefPubMedGoogle Scholar
  • 4 Van den Bosch H. Intracellular phospholipases. Biochim Biophys Acta 1980; 604: 191-246
    PubMedGoogle Scholar
  • 5 Slater TF. Free-radical mechanisms in tissue injury. Biochem J 1984; 222: 1-15
    CrossrefPubMedGoogle Scholar
  • 6 Benedetti A, Comporti M, Esterbauer H. Identification of 4-hydroxy-nonenal as a cytotoxic product originating from the peroxidation of liver microsomal lipids. Biochim Biophys Acta 1980; 620: 281-296
    CrossrefPubMedGoogle Scholar
  • 7 Dianzani MU. Biochemical effects of saturated and unsaturated aldehydes. In: Free Radicals, Lipid Peroxidation and Cancer McBrien DC H, Slater JF. (eds.) pp 129-158 Academic Press; London: 1982
    Google Scholar
  • 8 Curzio M, Torrielli MV, Giroud JP, Esterbauer H, Dianzani MU. Neutrophil chemotactic responses to aldehydes. Res Commun Chem Pathol Pharmacol 1982; 36: 463-476
    PubMedGoogle Scholar
  • 9 Jurgens G, Lang J, Esterbauer H. Modification of human low-density lipoprotein by the lipid peroxidation product 4-hydroxynonenal. Biochim Biophys Acta 1986; 875: 103-114
    CrossrefPubMedGoogle Scholar
  • 10 Brambilla G, Sciaba L, Faggin P, Maura A, Marinari UM, Ferro M, Esterbauer H. Cytotoxicity, DNA fragmentation and sister-chromatid exchange in Chinese hamster ovary cells exposed to the lipid peroxidation product 4-hydroxynonenal and homologous aldehydes. Mutat Res 1986; 171: 169-176
    CrossrefPubMedGoogle Scholar
  • 11 Esterbauer H. Aldehydic products of lipid peroxidation. In: Free Radicals, Lipid Peroxidation and Cancer. McBrien DC H, Slater TF. (eds.) Academic Press; London: 1982. pp 101-128
  • 12 Esterbauer H, Ertl A, Scholz N. The reaction of cysteine with α,β-unsaturated aldehydes. Tetrahedron 1976; 32: 285-289
    CrossrefPubMedGoogle Scholar
  • 13 Cadenas E, Muller A, Brigelius R, Esterbauer H, Sies H. Effects of 4-hydroxynonenal on isolated hepatocytes. Studies on chemiluminescence response, alkane production and glutathione status Biochem J 1983; 214: 479-487
    CrossrefPubMedGoogle Scholar
  • 14 Esterbauer H, Benedetti A, Lang J, Fulceri R, Faiiler G, Comporti M. Studies on the mechanism of formation of 4-hydroxynonenal during microsomal lipid peroxidation. Biochim Biophys Acta 1986; 876: 154-166
    CrossrefPubMedGoogle Scholar
  • 15 Esterbauer H, Cheeseman KH, Dianzani MU, Poli G, Slater TF. Separation and characterization of the aldehydic products of lipid peroxidation stimulated by ADP-Fe2+ in rat liver microsomes. Biochem J 1982; 208: 129-140
    CrossrefPubMedGoogle Scholar
  • 16 Cazenave JP, Packham MA, Mustard JF. Adherence of platelets to a collagen-coated surface: development of a quantitative model, j Lab Clin Med. 1973; 82: 978-990
    PubMedGoogle Scholar
  • 17 Cameron HA, Archie NG. The facilitating effects of adrenaline on platelet aggregation. Prostaglandins Leukotrienes Med 1982; 9: 117-128
    CrossrefPubMedGoogle Scholar
  • 18 Esterbauer H, Weger W. Über die Wirkungen von Aldehyden auf gesunde und maligne Zellen, 3. mitt: Synthese von homologen 4- hydroxy-2-alkenalen. II. Monatsh Chem 1967; 98: 1994-2000
    CrossrefPubMedGoogle Scholar
  • 19 Kannagi R, Koizumi K. Effect of different physical states of phospholipid substrates on partially purified platelet phospholipase A2activity. Biochim Biophys Acta 1979; 556: 423-433
    CrossrefPubMedGoogle Scholar
  • 20 Mauco G, Chap H, Douste-Blazy L. Characterization and properties of a phosphatidylinositol phosphodiesterase (phospholipase C) from platelet cytosol. FEBS Lett 1979; 100: 367-370
    CrossrefPubMedGoogle Scholar
  • 21 Vladimirov Yu A, Olenev VI, Suslova TB. Cheremisina Z R Lipid peroxidation in mitochondrial membrane. Adv Lipid Res 1980; 17: 173-249
    PubMedGoogle Scholar
  • 22 Esterbauer H, Roller E, Slee RG, Koster JF. Possible involvement of the lipid-peroxidation product 4-hydroxynonenal in the formation of fluorescent chromolipids. Biochem J 1986; 239: 405-409
    CrossrefPubMedGoogle Scholar
  • 23 Douglas CE, Chan AC, Choy C. Vitamin E inhibits platelet phospholipase A2 . Biochim Biophys Acta 1986; 876: 639-645
    CrossrefPubMedGoogle Scholar
  • 24 Yasuda M, Fujita T. Effect of lipid peroxidation on phospholipase A2activity of rat liver mitochondria. Jpn J Pharmacol 1977; 27: 429-435
    CrossrefPubMedGoogle Scholar
  • 25 Fujimoto Y, Tanioka H, Keshi I, Fujita T. The interaction between lipid peroxidation and prostaglandin synthesis in rabbit kidney medulla slices. Biochem J 1983; 212: 167-171
    CrossrefPubMedGoogle Scholar
  • 26 Sevanian A, Muakkassah-Kelly SF, Montestruque S. The influence of phospholipase A2 and glutathione peroxidase on the elimination of membrane lipid peroxides. Arch Biochem Biophys 1983; 223: 441-452
    CrossrefPubMedGoogle Scholar
  • 27 Tan KH, Meyer DJ, Belin J, Ketterer B. Inhibition of microsomal lipid peroxidation by glutathione and glutathione transferases B and AA. Role of endogenous phospholipase A? Biochem J 1984; 220: 243-252
    CrossrefPubMedGoogle Scholar
  • 28 Sevanian A, Kim E. Phospholipase A2 dependent release of fatty acids from peroxidized membranes. J Free Radic Biol Med 1985; 1: 263-271
    CrossrefPubMedGoogle Scholar
  • 29 Hemler ME, Cook HW, Lands WE M. Prostaglandin biosynthesis can be triggered by lipid peroxides. Arch Biochem Biophys 1979; 193: 340-355
    CrossrefPubMedGoogle Scholar
  • 30 Egan RW, Paxton J, Kuehl FA. Mechanism for irreversible selfdeactivation of prostaglandin synthetase. J Biol Chem 1976; 251: 7329-7335
    PubMedGoogle Scholar
  • 31 Silk ST, Wong KT H, Marcus AJ. Arachidonic acid releasing activity in platelet membranes: Effects of sulfhydryl-modifying agents. Biochemistry 1981; 20: 391-397
    CrossrefPubMedGoogle Scholar
  • 32 Graff G, Gellerman JL, Sand DM, Schlenk H. Inhibition of blood platelet aggregation by dioxo-ene compounds. Biochim Biophys Acta 1984; 799: 143-150
    CrossrefPubMedGoogle Scholar
  • 33 Yamada K, Shuto K, Nakamizo N. Inhibition of platelet aggregation by a new agent, 2,2’-dithiobis-(N-2-hydroxypropylbernzamide) (KF4939). Thromb Res 1983; 29: 197-206
    CrossrefPubMedGoogle Scholar
  • 34 Yamada K, Shuto K, Kubo K, Nakamizo N. Evidence that KF4939, a new anti-platelet agent, inhibits phospholipase activation in rabbit platelets: Different aspects from cyclic AMP increasing agents and calmodulin antagonist. Arch int Pharmacodyn Ther 1984; 268: 141-154
    PubMedGoogle Scholar
  • 35 Yamada K, Kubo K, Shuto K, Nakamizo N. Involvement of disulfide-sulfhydryl interaction in anti-platelet actions of KF4939. Thromb Res 1985; 38: 61-69
    CrossrefPubMedGoogle Scholar
  • 36 Lang J, Celotto C, Esterbauer H. Quantitative determination of the lipid peroxidation product 4-hydroxynonenal by high-performance liquid chromatography. Anal Biochem 1985; 150: 369-378
    CrossrefPubMedGoogle Scholar