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Thromb Haemost 1988; 59(02): 197-201
DOI: 10.1055/s-0038-1642753
Original Articles
Schattauer GmbH Stuttgart

Anticoagulant Activity in Cell Homogenate of Adult T Cell Leukemia

H Wada
The 2nd Division, Department of Internal Medicine, Faculty of Medicine, Mie University, Mie, Japan
,
M Tomeoku
The 2nd Division, Department of Internal Medicine, Faculty of Medicine, Mie University, Mie, Japan
,
A Deguchi
The 2nd Division, Department of Internal Medicine, Faculty of Medicine, Mie University, Mie, Japan
,
H Suzuki
The 2nd Division, Department of Internal Medicine, Faculty of Medicine, Mie University, Mie, Japan
,
Y Mori
The 2nd Division, Department of Internal Medicine, Faculty of Medicine, Mie University, Mie, Japan
,
M Ito
*   The Department of Obstetrics and Gynecology, Faculty of Medicine, Mie University, Mie, Japan
,
K Deguchi
The 2nd Division, Department of Internal Medicine, Faculty of Medicine, Mie University, Mie, Japan
,
S Shirakawa
The 2nd Division, Department of Internal Medicine, Faculty of Medicine, Mie University, Mie, Japan
› Author Affiliations
Further Information

Publication History

Received 12 August 1986

Accepted after revision 16 November 1987

Publication Date:
21 May 2018 (online)

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Summary

The hemostatic abnormality in 18 patients with adult T cell leukemia (ATL) was studied. Activated partial thromboplastin time (APTT) was slightly prolonged and prekallikrein activity was markedly low in these patients. The leukemic cell homogenate from these patients prolonged the recalcification time (RCT) of normal plasma; homogenates containing more than 3 ×103 cells/μi prolonged it, although a lower cell concentration shortened it. The crude anticoagulant fraction from the gel filtration, with a molecular weight of about 34,000, prolonged RCT. The crude anticoagulant did not affect prothrombin time (PT), thrombin activity or activated X activity at any concentration, but prolonged the contact activation test, inhibited the activation of prekallikrein and prolonged RCT of Fletcher trait, Fitzgerald trait and F XII deficient plasma. These effects of ATL cell homo-genate were stronger on platelet poor plasma than on platelet rich plasma. Although ATL cells had low procoagulant activity, increase of leukemic cells made anticoagulant activity predominant, might be the cause of hemostatic abnormality or amplify the bleeding tendency in patients with ATL.

Keywords

Anticoagulant activity - Adult T cell leukemia (ATL) - Leukemic cell homogenate - Prekallikrein - Contact phase of coagulation system
 

  • References

  • 1 Gralnick HR, Bargley J, Abrell E. Heparin treatment for hemorrhagic diathesis of acute promyelocytic leukemia. Amer J Med 1972; 52: 167-174
    CrossrefPubMedGoogle Scholar
  • 2 Ogston D, Dawson AA, Adam HH. The fibrinolytic enzyme system in leukemia, myelomatosis and myeloproliferative disease. Acta Haemat 1972; 48: 323-330
    PubMedGoogle Scholar
  • 3 Rosenthal RL. Acute promyelocytic leukemia associated with hypofibrinogenemia. Blood 1962; 21: 495-508
    PubMedGoogle Scholar
  • 4 Gralnick HR. Cancer cell procoagulant activity. Malignancy and Hemostatic system. Donati MB. (ed.) Raven Press; New York: 1981: 57-61
    Google Scholar
  • 5 O’Meara RA Q. Coagulative properties of cancer. Irish J Med Sci 1958; 394: 474-485
    PubMedGoogle Scholar
  • 6 Gralnick HR, Abrell E. Studies on the procoagulant and fibrinolytic activity of promyelocytes in acute promyelocytic leukemia. Brit J Haematol 1973; 24: 89-98
    CrossrefPubMedGoogle Scholar
  • 7 Heilmann MG, Chardon E, Sulton C, Josso F. The procoagulant factor of leukemic promyelocytes. Brit J Haematol 1975; 30: 151-158
    CrossrefPubMedGoogle Scholar
  • 8 Wada H, Nagano T, Tomeoku M, Kuto M, Karitani Y, Deguchi K, Shirakawa S. Coagulant and fibrinolytic activities in the leukemic cell lysates. Thromb Res 1982; 30: 315-322
    PubMedGoogle Scholar
  • 9 Gordon SG, Franks JJ, Levis B. Cancer procoagulant A; Factor X activating procoagulant from malignant tissue. Thromb Res 1976; 8: 263-273
    CrossrefPubMedGoogle Scholar
  • 10 Sakuragawa N, Takahashi K, Hoshiyama M, Jimbo C, Matsuoka M, Onishi Y. Pathologic cells as procoagulant substance of disseminated intravascular coagulation syndrome in acute promyelocytic leukemia. Thromb Res 1976; 8: 263-273
    CrossrefPubMedGoogle Scholar
  • 11 Varadi K, Marossy K, Asboth G, Elodi P, Elodi S. Inactivation of human factor VIII by granulocyte protease. Thromb Haemostas 1980; 43: 45-48
    PubMedGoogle Scholar
  • 12 Uchiyama T, Yodoi J, Sagawa K, Takatsuki K, Uchino H. Adult T cell leukemia-Clinical and hematological features of 16 cases. Blood 1977; 50: 481-492
    PubMedGoogle Scholar
  • 13 Tomeoku M, Kuto M, Nagano T, Wada H, Murashima S, Ito T, Lee C-J, Morito T, Konishi M, Karitani Y, Deguchi K, Shirakawa S. Chemotherapy and disseminated intravascular coagulation in hematopoietic malignancy. J Clin Hemat 1982; 32: 1400-1410
    PubMedGoogle Scholar
  • 14 Langdell PD, Wagner RH, Brinkhous KM. Effect of antihemophilic factor on one-stage clotting time. J Lab Clin Med 1953; 47: 637-647
    PubMedGoogle Scholar
  • 15 Quick AJ, Brown MS, Bancroft FW. A study of the coagulation defect in hemophilia and in jaundice. Am J Med Sci 1935; 190: 501-511
    CrossrefPubMedGoogle Scholar
  • 16 Ferreira HC, Murat LG. An immunological method for demonstrating fibrin degradation products in serum and its use in the diagnosis of fibrinolytic states. Brit J Haematol 1963; 9: 299-310
    CrossrefPubMedGoogle Scholar
  • 17 Foster JN T, Denatale A, Dotti LB. Determination of plasma fibrinogen by means of centrifugation after heating. Am J Clin Pathol 1959; 31: 42-5
    CrossrefPubMedGoogle Scholar
  • 18 Kaulla KN von, Schultz RL. Methods for the evaluation of human fibrinolysis. Am J Clin Path 1958; 29: 104-109
    CrossrefPubMedGoogle Scholar
  • 19 Colman RT, Robboy SJ, Minna JD. Disseminated intravascular coaculation (DIC); An approach. Am J Med 1974; 52: 679-89
    PubMedGoogle Scholar
  • 20 Catovsky D, Greaves MF, Rose M, Galton DA G, Goolden AW G, McCluskey DR, White JM, Lampert I, Bourikas G, Ireland R, Brownell AI, Bridger JM, Blattner WA, Gallo RC. Adult T cell lymphoma-leukemia in blacks from the west Indies. Lancet 1982; 1: 639-641
    PubMedGoogle Scholar
  • 21 Osterud B, Rapaport SI. Activation of factor IX by the reaction product of tissue factor and factor VII: Additional pathway for initiating blood coagulation. Proc Natl Acad Sci 1977; 74: 5260-5264
    CrossrefPubMedGoogle Scholar
  • 22 Walsh PN. The effect of collagen and kaolin on the intrinsic coagulation activity of platelets. Br J Haematol 1972; 22: 393-405
    CrossrefPubMedGoogle Scholar