Epidermal Growth Factor Stimulates Prostacyclin Production by Cultured Human Vascular Endothelial Cells

Summary

Epidermal growth factor (EGF) stimulated prostacyclin (PGI₂) production by cultured human umbilical vein endothelial cells, as measured by radioimmunoassay of its stable metabolite 6-keto- prostaglandin F_lα. This effect of EGF was dose-dependent, the lowest stimulatory concentration of EGF was 1.0 ng/ml and 100 ng/ml caused a 2.7 ± 0.3 (mean ± SEM) fold increase in the PGI₂ synthesis. The stimulation appeared at 3-6 h of incubation and lasted at least 24 h. It was suppressed by EGF antibodies and blocked by protein synthesis inhibitor cycloheximide. Cells preincubated 12 h with EGF released also higher amounts of PGI₂when incubated with thrombin for 5 min. It is concluded that EGF liberated from platelets during aggregation may prevent local thrombogenesis and atherogenesis by stimulating the release of the antiaggregatory, vasodilatory PGI₂ from vascular endothelial cells.

Keywords

(Human) Endothelial cells - Epidermal growth factor - Prostacyclin

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