Thrombosis and Haemostasis, Inhaltsverzeichnis Thromb Haemost 1988; 59(02): 248-250DOI: 10.1055/s-0038-1642763 Original Articles Schattauer GmbH Stuttgart Epidermal Growth Factor Stimulates Prostacyclin Production by Cultured Human Vascular Endothelial Cells Ari Ristimäki 1 Children's Hospital, University of Helsinki, Helsinki, Finland , Olavi Ylikorkala 2 The Department of Obstetrics and Gynecology, University of Helsinki, Helsinki, Finland , Jaakko Perheentupa 1 Children's Hospital, University of Helsinki, Helsinki, Finland , Lasse Viinikka 1 Children's Hospital, University of Helsinki, Helsinki, Finland › Institutsangaben Artikel empfehlen Abstract als PDF herunterladen Artikel einzeln kaufen Summary Epidermal growth factor (EGF) stimulated prostacyclin (PGI2) production by cultured human umbilical vein endothelial cells, as measured by radioimmunoassay of its stable metabolite 6-keto- prostaglandin Flα. This effect of EGF was dose-dependent, the lowest stimulatory concentration of EGF was 1.0 ng/ml and 100 ng/ml caused a 2.7 ± 0.3 (mean ± SEM) fold increase in the PGI2 synthesis. The stimulation appeared at 3-6 h of incubation and lasted at least 24 h. It was suppressed by EGF antibodies and blocked by protein synthesis inhibitor cycloheximide. Cells preincubated 12 h with EGF released also higher amounts of PGI2when incubated with thrombin for 5 min. It is concluded that EGF liberated from platelets during aggregation may prevent local thrombogenesis and atherogenesis by stimulating the release of the antiaggregatory, vasodilatory PGI2 from vascular endothelial cells. Keywords Keywords(Human) Endothelial cells - Epidermal growth factor - Prostacyclin PDF (232 kb) Referenzen References 1 Moncada S, Vane JR. Pharmacology and endogenous roles of prostaglandin endoperoxides, thromboxane A2, and prostacyclin. Pharmacol Rev 1979; 30: 293-331 2 Ross R. 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