Cytomegalovirus Alters the von Willebrand Factor Content in Human Endothelial Cells

Summary

Cultured human endothelial cells were infected with human cytomegalovirus AD 169 and Kerr. The infection resulted in the appearance of viral antigens in the nuclei of about 10% of the endothelial cells and in the concomittant disappearance of vWF from the infected cells. No differences were observed between endothelial cells from different sources (umbilical cord veins or arteries, adult veins).

Keywords

von - Willebrand factor - Cytomegalovirus - Endothelial cells

• References

• 1 McGill HC. Persistent problems in the pathogenesis of atherosclerosis. Arteriosclerosis 1984; 4: 443-451
• 2 Majno G, Joris I, Zand T. Atherosclerosis: new horizons. Human Pathology 1985; 16: 3-5
• 3 Benditt EP, Barrett T, McDougall JK. Viruses in the etiology of atherosclerosis. Proc Natl Acad Sci. USA 1983; 80: 6386-6389
• 4 Melnick JL, Dreesman GR, McCollum CH, Petrie BL, Burek J, DeBakey ME. Cytomegalovirus antigen within human arterial smooth muscle cells. Lancet 1983; 2: 644-647
• 5 Reinders JH, Groot P G de, Dawes J, Hunter NR, Heugten HA A van, Zandbergen J, Gonsalves MD, Mourik J A van. Comparison of secretion and subcellular localization of von Willebrand protein with that of thrombospondin and fibronectin in cultured human vascular endothelial cells. Biochem Biophys Acta 1985; 844: 306-313
• 6 Wachem P B van, Reinders JH, Buul van Wortelboer M F, Groot P G de, Aken W G van, Mourik J A van. Von Willebrand factor in cultured human vascular endothelial cells from adult and umbilical cord arteries and veins. Thromb Haemostas 1986; 56: 189-192
• 7 Jaffe EA, Hoyer LW, Nachmann RL. Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J Clin Invest 1973; 52: 2757-2764
• 8 Maciag T, Cerundolo J, Ilsley S, Kelley PR, Foriand R. An endothelial cell growth factor from bovine hypothalamus: Identification and partial characterization. Proc Nat Acad Sci. USA 1979; 76: 5674-5678
• 9 Voyta JC, Via DP, Butterfield CE, Zetter BR. Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein. J Cell Biol 1984; 99: 2034-2040
• 10 Hendrix MGR, Bruggeman CA, Boven CP A van. Alterations of the functional state of peritoneal macrophages during rat cytomegalovirus infection in vivo. FEMS Microbiol Lett 1986; 33: 111-115
• 11 Bruggeman CA, Debie WH M, Grauls G, Boven CP A van. Cytomegalovirus infection of rat endothelial cells in vitro. Arch Virol 1986; 87: 265-272
• 12 Kim KS, Sapienza VJ, Chen CM J, Wisniewski K. Production and characterization of monoclonal antibodies specific for a glycosylated polypeptide of human cytomegalovirus. J Clin Microbiol 1983; 18: 331-343
• 13 Laurila P, Virtanen I, Wartiovaara J, Stenman S. Fluorescent antibodies and lectins strain intracellular structures in fixed cells treated with nonionic detergent. J Histochem Cytochem 1978; 26: 251-257
• 14 Goding JW. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods 1976; 13: 215-226
• 15 Dam-Mieras MC E van, Muller AD, Dieijen G van, Hemker HC. Blood coagulation enzymes, the factors II, V, VII, VIII, X and XI. Determination by clotting assays. p 352-394 In: Methods of enzymatic analysis Bergmeijer HU, Bergmeijer J, Grassl M. (eds.) Vol.5 Verlag Chemie GmbH; Weinheim, FRG: 1984
• 16 Lawrell CB. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem 1966; 15: 45-52
• 17 Blaes N, Piovella F, Samaden A, Boutherin-Falson O, Ricetti M. Nicotine alters fibronectin and factor VIII/vWF in human vascular endothelial cells. Br J Haematol 1986; 64: 675-687
• 18 Holthöfer H, Virtanen J, Kariniemi A-L, Hormia M, Linder E, Miettinen A. Ulex Europaeus I Lectin as a marker for vascular endothelium in human tissues. Lab Invest 1982; 47: 60-66
• 19 Keller R, Peitchel R, Goldman JN, Goldman M. An IgG-Fc receptor induced in cytomegalovirus-infected human fibroblasts. J Immunol 1976; 116: 772-777
• 20 Bruggeman CA, Reinders R, Debie WM H, Frederik PM, Engels W, Boven CP A van. Demonstration of an IgG-Fc receptor in rat cytomegalovirus infected cells. Arch Virol 1984; 81: 79-89
• 21 Ho HD, Rota TR, Andrews CA, Hirsch MS. Replication of human cytomegalovirus in endothelial cells. J Infect Dis 1984; 150: 956-957
• 22 Banji A, Salisbury R. Cytomegalovirus and vasculitis. Brit Med J 1978; 1: 623-624
• 23 Myerson D, Hackman RC, Nelson JA, Ward DC, McDougall JK. Widespread presence of histologically occult cytomegalovirus. Hum Pathol 1984; 15: 430-439
• 24 Mims CA, Gould J. Infection of salivary glands, kidneys, adrenals, ovaries and epithelial by murine cytomegalovirus. J Med Microbiol 1979; 12: 113-122
• 25 MacGregor RR, Friedman HM, Macarak EJ, Kefalides NA. Virus infection of endothelial cells increases granulocyte adherence. J Clin Invest 1980; 65: 1469-1477
• 26 Sacks T, Moldow CF, Craddock PR, Bowers TK, Jacob HS. Oxygen radicals mediate endothelial cell damage by complement-stimulated granulocytes. J Clin Invest 1978; 61: 1161-1167
• 27 Dam-Mieras MCE van, Bruggeman CA, Muller AD, Debie WH M, Zwaal RF A. Induction of endothelial cell procoagulant activity by cytomegalovirus infection. Thrombosis Research 1987; 47: 69-75
• 28 Meyer H, Dormans PH J, Boven CP A van. Studies on rat cytomegalovirus induced structural and non structural proteins present at (immediate) early and late times of infection. Arch Virol 1986; 89: 45-56
• 29 DeMarchi JM. Post-nonscriptional control of human cytomegalovirus gene expression. Virology 1983; 124: 390-402
• 30 Hormia M, Lehro VP, Virtanen I. Factor VUI-related antigen. A pericellular matrix component of cultured human endothelial cells. Exp Cell Res 1983; 149: 483-497