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Thromb Haemost 1988; 59(02): 299-303
DOI: 10.1055/s-0038-1642775
Original Articles
Schattauer GmbH Stuttgart

Fibrinolysis in Normal Subjects - Comparison Between Plasminogen Activator Inhibitor and Other Components of the Fibrinolytic System

Grazia Nicoloso
The Division d’;HématoIogie, Däpartement de Médecine Interne, CHUV, Lausanne, Lausanne, Switzerland
,
Jacques Hauert
The Division d’;HématoIogie, Däpartement de Médecine Interne, CHUV, Lausanne, Lausanne, Switzerland
,
Egbert K O Kruithof
The Division d’;HématoIogie, Däpartement de Médecine Interne, CHUV, Lausanne, Lausanne, Switzerland
,
Guy Van Melle
*   Lausanne, and the Institut de Médecine Sociale et Préventive, Lausanne, Switzerland
,
Fedor Bachmann
The Division d’;HématoIogie, Däpartement de Médecine Interne, CHUV, Lausanne, Lausanne, Switzerland
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Publikationsverlauf

Received 20. März 1987

Accepted after revision 24. Dezember 1987

Publikationsdatum:
21. Mai 2018 (online)

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Summary

We analyzed fibrinolytic parameters in 20 healthy men and 20 healthy women, aged from 25 to 59, before and after 10 and 20 min venous occlusion. The 10 min post-occlusion fibrinolytic activity measured directly in diluted unfractionated plasma by a highly sensitive 125I-fibrin plate assay correlated well with the activity of euglobulins determined by the classical fibrin plate assay (r = 0.729), but pre-stasis activities determined with these two methods did not correlate (r = 0.084). The enhancement of fibrinolytic activity after venous occlusion was mainly due to an increase of t-PA in the occluded vessels (4-fold increase t-PA antigen after 10 min and 8-fold after 20 min venous occlusion). Plasminogen activator inhibitor (PAI) activity and plasminogen activator inhibitor 1 (PAI-1)1 antigen levels at rest showed considerable dispersion ranging from 1.9 to 12.4 U/ml, respectively 6.9 to 77 ng/ml. A significant increase of PAI-1 antigen levels was observed after 10 and 20 min venous occlusion. At rest no correlation was found between PAI activity or PAI-1 antigen levels and the fibrinolytic activity measured by 125I-FPA. However, a high level of PAI-1 at rest was associated with a high prestasis antigen level of t-PA and a low fibrinolytic response after 10 min of venous stasis. Since the fibrinolytic response inversely correlated with PAI activity at rest, we conclude that its degree depends mainly on the presence of free PAI.

Keywords

Fibrinolysis - Tissue-type plasminogen activator - Urokinase - Plasminogen activator inhibitor - PAI - PAI-1
 

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