Expression of Fibrinogen on the Surface of ADP-Stimulated Platelets: Comparison of Human and Rabbit Platelets

Summary

Fibrinogen is a cofactor in the aggregation of human platelets and must be added to suspensions of washed human platelets forextensive aggregation to occur in response to ADR Although thepatterns of fibrinogen binding and dissociation during plateletaggregation and deaggregation are similar for human and rabbitplatelets, washed rabbit platelets aggregate extensively whenstimulated with ADP even in the absence of added fibrinogen. Todetermine whether secreted fibrinogen was present on the surfaceof ADP-stimulated platelets and available to support aggregation, the binding of ¹²⁵I-F(ab’)₂ fragments of anti-fibrinogen antibodiesto formaldehyde-fixed platelets was measured. Although nofibrinogen was detected on human platelets either before or aftertreatment with ADP, fibrinogen was expressed on the surface ofrabbit platelets after stimulation with ADP. However, thissecreted fibrinogen did not dissociate during deaggregation of theplatelets. Thus, the aggregation of rabbit platelets in the absenceof exogeneous fibrinogen may be supported by secreted fibrino-gen, but a fibrinogen-independent component has not beencompletely ruled out.

Keywords

Human platelets - Rabbit platelets - Fibrinogen - ADP

• References

• 1 Bennett JS. The platelet-fibrinogen interaction. In: Platelet Membrane Glycoproteins George JN, Nurden AT, Phillips DR. (eds).pp 193-214 Plenum Press; New York: 1985
• 2 Peerschke EI B. The platelet fibrinogen receptor. Semin Hematol 1985; 22: 241-259
• 3 Mustard JF, Packham MA, Kinlough-Rathbone RL, Perry DW, Regoeczi E. Fibrinogen and ADP-induced platelet aggregation. Blood 1978; 52: 453-466
• 4 Bennett JS, Vilaire G. Exposure of platelet fibrinogen receptors by ADP and epinephrine. J Clin Invest 1979; 64: 1393-1401
• 5 Marguerie GA, Edgington TS, Plow EF. Interaction of fibrinogen with its platelet receptor as part of a multistep reaction in ADP- induced platelet aggregation. J Biol Chem 1980; 255: 154-161
• 6 Peerschke EI, Zucker MB, Grant RA, Egan JJ, Johnson MM. Correlation between fibrinogen binding to human platelets and platelet aggregability. Blood 1980; 55: 841-847
• 7 Harfenist EJ, Packham MA, Mustard JF. Reversibility of the association of fibrinogen with rabbit platelets exposed to ADP.. Blood 1980; 56: 189-198
• 8 Mustard JF, Perry DW, Ardlie NG, Packham MA. Preparation of suspensions of washed platelets from humans. Br J Haematol 1972; 22: 193-204
• 9 Harfenist EJ, Wrana JL, Packham MA, Mustard JF. Measurement of fibrinogen concentrations in suspensions of washed rabbit and human platelets by radioimmunoassays. Thromb Haemostas 1985; 53: 110-115
• 10 Ardlie NG, Packham MA, Mustard JF. Adenosine diphosphate- induced platelet aggregation in suspensions of washed rabbit platelets. Br J Haematol 1970; 19: 7-17
• 11 Regoeczi E. Fibrinogen catabolism: Kinetics of catabolism following a sudden elevation of the pool with exogenous fibrinogen. Clin Sci 1970; 38: 111-121
• 12 Johnstone A, Thorpe R. Immunochemistry in Practice. pp 52-56 Blackwell, Oxford 1982;
• 13 Molnar J, Lorand L. Studies on apyrases. Arch Biochem Biophys 1961; 93: 353-63
• 14 McFarlane AS. Efficient trace-labelling of proteins with iodine. Nature 1958; 182: 53-53
• 15 Kinlough-Rathbone RL, Mustard JF, Packham MA, Perry DW, Reimers H-J, Cazenave J-P. Properties of washed human platelets. Thromb Haemostas 1977; 37: 291-308
• 16 Ardlie NG, Perry DW, Packham MA, Mustard JF. Influence of apyrase on stability of suspensions of washed rabbit platelets. Proc Soc Exp Biol Med 1971; 136: 1021-1023
• 17 Harfenist EJ, Guccione MA, Packham MA, Mustard JF. The use of the synthesic peptide, Gly-Pro-Arg-Pro, in the preparation of throm- bin-degranulated rabbit platelets. Blood 1982; 59: 952-955
• 18 Harfenist EJ, Guccione MA, Packham MA, Kinlough-Rathbone RL, Mustard JF. Arachidonate-induced fibrinogen binding to throm- bin-degranulated rabbit platelets is independent of released ADP. Blood 1982; 59: 956-962
• 19 Ginsberg MH, Plow EF. Fibronectin expression on the platelet surface occurs in concert with secretion. J Supramol Struct Cell Biochem 1981; 17: 91-98
• 20 Stenberg PE, Shuman MA, Levine SP, Bainton DF. Redistribution of alpha-granules and their contents in thrombin-stimulated platelets. J Cell Biol 1984; 98: 748-760
• 21 Wencel-Drake JD, Plow EF, Zimmerman TS, Painter RG, Ginsberg MH. Immunofluorescent localization of adhesive glyco- proteins in resting and thrombin-stimulated platelets. Am J Pathol 1984; 115: 156-164
• 22 Asch A, Leung LL K, Polley MJ, Nachman RL. Platelet membrane topography: Colocalization of thrombospondin and fibrinogen with the glycoprotein Ilb-IIIa complex. Blood 1985; 66: 926-934
• 23 Hourdille P, Hasitz M, Belloc F, Nurden AT. Immunocytochemical study of the binding of fibrinogen and thrombospondin to ADP- and thrombin-stimulated human platelets. Blood 1985; 65: 912-920
• 24 Isenberg WM, McEver RP, Phillips DR, Shuman MA, Bainton DF. The platelet fibrinogen receptor: An immunogold-surface replica study of agonist-induced ligand binding and receptor clustering. J Cell Biol 1987; 104: 1655-1663
• 25 Suzuki H, Kinlough-Rathbone RL, Packham MA, Tanoue K, Yamazaki H, Mustard JF. Immunocytochemical localization of fibrinogen during thrombin-induced aggregation of human platelets. Thromb Haemostas 1987; 58: 291 (Abstr)
• 26 Cattaneo M, Kinlough-Rathbone RL, Lecchi A, Bevicqua C, Packham MA, Mustard JF. Fibrinogen-independent aggregation and deaggregation of human platelets: Studies in two afibrinogenemic patients. Blood 1987; 70: 221-226
• 27 Zucker MB. Relationship of plasma clotting factors to platelets. Excejpta Medica 1974; International Congress Series 415, Topics in Hematology: 280-280
• 28 Courtois G, Ryckewaert J-J, Woods VL, Ginsberg MH, Plow EF, Marguerie GA. Expression of intracellular fibrinogen on the surface of stimulated platelets. Eur J Biochem 1986; 159: 61-67
• 29 Legrand C, Dubernard V, Nurden AT. A comparison of the surface expression of platelet fibrinogen with the binding of plasma fibrinogen to activated platelets. Thromb Res 1986; Suppl (Suppl. 01) VI 71 Abstr 141-141
• 30 Suzuki H, Kinlough-Rathbone RL, Packham MA, Tanoue K, Yamazaki H, Mustard JF. Immunocytochemical localization of fibrinogen during ADP-induced aggregation of human platelets. FedProc 1987; 46: 2381-2381 (Abstr)
• 31 Harfenist EJ, Packham MA, Kinlough-Rathbone RL, Mustard JF. Inhibitors of ADP-induced platelet aggregation prevent fibrinogen binding to rabbit platelets and cause rapid deaggregation and dissociation of bound fibrinogen. J Lab Clin Med 1981; 97: 680-688
• 32 Morgenstern E, Edelman L, Reimers H-J, Miyashita C, Haurand M. Fibrinogen distribution on surfaces and in organelles of ADP-stimulated human blood platelets. Eur J Cell Biol 1985; 38: 292-300