Expression of Fibrinogen on the Surface of ADP-Stimulated Platelets: Comparison of Human and Rabbit Platelets

Elizabeth J Harfenist; Marian A Packham; Fraser J Mustard

doi:10.1055/s-0038-1642779

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1988; 59(02): 319-322
DOI: 10.1055/s-0038-1642779

Original Articles

Schattauer GmbH Stuttgart

Expression of Fibrinogen on the Surface of ADP-Stimulated Platelets: Comparison of Human and Rabbit Platelets

Authors

Elizabeth J Harfenist

The Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
Marian A Packham

The Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
Fraser J Mustard

^*Department of Pathology, McMaster University, Hamilton, Ontario, Canada

Abstract

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Summary

Fibrinogen is a cofactor in the aggregation of human platelets and must be added to suspensions of washed human platelets forextensive aggregation to occur in response to ADR Although thepatterns of fibrinogen binding and dissociation during plateletaggregation and deaggregation are similar for human and rabbitplatelets, washed rabbit platelets aggregate extensively whenstimulated with ADP even in the absence of added fibrinogen. Todetermine whether secreted fibrinogen was present on the surfaceof ADP-stimulated platelets and available to support aggregation, the binding of ¹²⁵I-F(ab’)₂ fragments of anti-fibrinogen antibodiesto formaldehyde-fixed platelets was measured. Although nofibrinogen was detected on human platelets either before or aftertreatment with ADP, fibrinogen was expressed on the surface ofrabbit platelets after stimulation with ADP. However, thissecreted fibrinogen did not dissociate during deaggregation of theplatelets. Thus, the aggregation of rabbit platelets in the absenceof exogeneous fibrinogen may be supported by secreted fibrino-gen, but a fibrinogen-independent component has not beencompletely ruled out.

Keywords

Human platelets - Rabbit platelets - Fibrinogen - ADP

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Referenzen

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