Klin Padiatr 2018; 230(03): 169
DOI: 10.1055/s-0038-1645000
Georg Thieme Verlag KG Stuttgart · New York
Circular RNAs in Neuroblastoma
C Danßmann
1
Charité – Universitätsmedizin Berlin
,
J Toedling
1
Charité – Universitätsmedizin Berlin
3
German Cancer Consortium (DKTK), Berlin
4
German Cancer Research Center (DKFZ), Heidelberg; * Authors contributed equally
,
F Klironomos
1
Charité – Universitätsmedizin Berlin
,
A Winkler
1
Charité – Universitätsmedizin Berlin
,
F Hertwig
1
Charité – Universitätsmedizin Berlin
3
German Cancer Consortium (DKTK), Berlin
4
German Cancer Research Center (DKFZ), Heidelberg; * Authors contributed equally
,
A Eggert
1
Charité – Universitätsmedizin Berlin
2
Berlin Institute of Health (BIH)
3
German Cancer Consortium (DKTK), Berlin
4
German Cancer Research Center (DKFZ), Heidelberg; * Authors contributed equally
,
JH Schulte
1
Charité – Universitätsmedizin Berlin
2
Berlin Institute of Health (BIH)
3
German Cancer Consortium (DKTK), Berlin
4
German Cancer Research Center (DKFZ), Heidelberg; * Authors contributed equally
,
S Fuchs
1
Charité – Universitätsmedizin Berlin
2
Berlin Institute of Health (BIH)
3
German Cancer Consortium (DKTK), Berlin
4
German Cancer Research Center (DKFZ), Heidelberg; * Authors contributed equally
› Author Affiliations
Circular RNAs (circRNA) are a class of non-coding RNA, which originate from back-splicing events during mRNA processing. They are highly abundant in neural tissues and play a role in cancer biology. This project investigates the role of circRNAs in neuroblastoma, the most common extracranial solid tumor of childhood.
We analyzed preliminary RNA sequencing data to identify circRNAs in neuroblastoma. A selection of identified circRNAs was validated in a panel of neuroblastoma cell lines, using qRT-PCR, RNaseR treatment, Sanger sequencing and northern blotting.
We identified 959 circRNAs in the neuroblastoma cell line IMR-5/75, of which many were derived from chromatin-modifying genes. A selected number of these circRNAs was validated.
Overall, we find circRNA expression to be uncorrelated with the host gene mRNA expression, suggesting an independent mechanism regulating circRNAs biogenesis in neuroblastoma.
