The Simultaneous Acute Release of Tissue-Type Plasminogen Activator and von Willebrand Factor in the Perfused Rat Hindleg Region

N Tranquille; J J Emeis

doi:10.1055/s-0038-1645065

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1990; 63(03): 454-458
DOI: 10.1055/s-0038-1645065

Original Article

Schattauer GmbH Stuttgart

The Simultaneous Acute Release of Tissue-Type Plasminogen Activator and von Willebrand Factor in the Perfused Rat Hindleg Region

N Tranquille

The Gaubius Institute TNO, Leiden, The Netherlands

,

J J Emeis

The Gaubius Institute TNO, Leiden, The Netherlands

› Author Affiliations

Abstract

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Summary

In perfused rat hindlegs, platelet-activating factor and bradyki-nin induced the acute release of both tissue-type plasminogen activator (t-PA) and von Willebrand Factor (vWF). The time course of release was similar for both proteins, and the amounts of t-PA and vWF released under various conditions were closely correlated. Release of both t-PA and vWF required extracellular calcium, and could be induced by the calcium ionophore A-23187. Protein synthesis was not required for release to occur.

Phorbol myristate acetate also induced release of t-PA and vWF, though with a different time course; DDAVP was inactive.

The results suggest that the release of t-PA, and that of vWF, are closely linked at the cellular level.

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References

References
1 Wagner DD, Olmsted JB, Marder VJ. Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells. J Cell Biol 1982; 95: 355-60
2 Reinders JH, De Groot PG, Gonsalves MD, Zandbergen J, Loesberg C, Van Mourik JA. Isolation of a storage and secretory organelle containing von Willebrand protein from cultured human endothelial cells. Biochim Biophys Acta 1984; 804: 361-9
3 Ewenstein BM, Warhol MJ, Handin RI, Pober JS. Composition of the von Willebrand factor storage organelle (Weibel-Palade body) isolated from cultured human umbilical vein endothelial cells. J Cell Biol 1987; 104: 1423-33
4 Mannucci PM, Gagnatelli G, D’Alonzor R. Stress and blood coagulation. Thromb Diath Haemorrh 1982; 51: 105-13
5 Giddings JC. The control of intravascular blood coagulation and haemostasis at endothelial surfaces. In: Biochemical Interactions at the Endothelium Cryer A. (ed) Elsevier Science Publishers; Amsterdam: 1983: 167-206
6 Levine JD, Marker L, Joseph ML, Counts RB. Thrombin-mediated release of factor VIII antigen from human umbilical vein endothelial cells in culture. Blood 1982; 531-4
7 Loesberg C, Gonsalves MD, Zandbergen J, Willems C, Van Aken WG, Stel HV, Van Mourik JA, De Groot PG. The effect of calcium on the secretion of factor Vlll-related antigen by cultured human endothelial cells. Biochim Biophys Acta 1983; 763: 160-8
8 De Groot PG, Gonsalves MD, Loesberg C, Van Buul-Wortelboer MF, Van Aken WG, Van Mourik JA. Thrombin-induced release of von Willebrand factor from endothelial cells is mediated by phospholipid methylation. J Biol Chem 1984; 259: 13329-33
9 Reinders JH, De Groot PG, Dawes J, Hunter NR, Van Heugten AH A, Zandbergen J, Gonsalves MO, Van Mourik JA. Comparison of secretion and subcellular localisation of von Willebrand protein with that of thrombospondin and fibronectin in cultured human vascular endothelial cells. Biochim Biophys Acta 1985; 844: 306-13
10 McArthur MM, MacGregor IR, Prowse CV, Hunter YZ, Dawes J, Pepper DS. The use of human endothelial cells cultured in flat wells and on microcarrier beads to assess tissue plasminogen activator and factor VIII related antigen release. Thromb Res 1986; 41: 581-7
11 Hamilton KK, Sims PJ. Changes in cytosolic Ca++ associated with von Willebrand factor release in human endothelial cells exposed to histamine. J Clin Invest 1987; 79: 600-8
12 Booth F, Allington MJ, Cederholm-Williams SA. An in vitro model for the study of acute release of von Willebrand factor from human endothelial cells. Br J Haematol 1987; 67: 71-8
13 Booth F, Marshall JM, Cederholm-Williams SA. Effect of bradykinin on the exposure of tissue plasminogen activator from human endothelial cells. Fibrinolysis 1988; 2: 107-10
14 Schorer AF, Moldow CF, Rick ME. Interleukin 1 or endotoxin increases the release of von Willebrand factor from human endothelial cells. Br J Haematol 1987; 67: 193-7
15 Ribes JA, Francis CW, Wagner DD. Fibrin induces release of von Willebrand factor from endothelial cells. I Clin Invest 1987; 79: 117-23
16 Prowse CV, MacGregor IR. Regulation of the plasminogen activator level in blood. In: Tissue-type Plasminogen Activator, Physiological and Clinical Aspects Kluft C. (ed) CRC Press; Boca Raton, FL: 1988. 2 49-65
17 Emeis JJ. Mechanisms involved in short-term changes in blood levels of tissue-type plasminogen activator. In: Tissue-type Plasminogen Activator, Physiological and Clinical Aspects Kluft C. (ed) CRC Press; Boca Raton, FL: 1988. 2 21-35
18 Emeis JJ. Perfused rat hindlegs. A model to study plasminogen activator release. Thromb Res 1983; 30: 195-203
19 Emeis JJ, Kluft C. PAF-acether-induced release of tissue-type plasminogen activator from vessel walls. Blood 1985; 66: 86-91
20 Tranquille N, Emeis JJ. Release of tissue-type plasminogen activator is induced in rats by leukotrienes C4 and D4, but not by prostaglandins El, E2 an 12. Br J Pharmacol 1988; 93: 156-64
21 Cash JD, Gader AA M, Da Costa J. The release of plasminogen activator and factor VIII to lysine vasopressin, arginine vasopressin, l-desamino-8-D-arginine vasopressin, angiotensin and oxytocin in man. Br J Haematol 1974; 27: 363-4
22 Mannucci PM, Aberg M, Nilsson IM, Robertson B. Mechanism of plasminogen activator and factor VIII increase after vasoactive drugs. Br J Haematol 1975; 30: 81-93
23 Marsh N, Gaffney P. Some observations on the release of extrinsic and intrinsic plasminogen activators during exercise in man. Haemostasis 1980; 9: 238-47
24 Nilsson IM, Vilhardt H, Holmberg L, Åstedt B. Association between factor VIII related antigen and plasminogen activator. Acta Med Scand 1982; 211: 105-12
25 Prowse CV, Farrugia A, Boulton FE, Tucker J, Ludlam CA, McLaren M, Belch FJ J, Prentice CRM, Dawes J, MacGregor IR. A comparative study using immunological and biological assays of the haemostatic responses to DDAVP infusion, venous occlusion and exercise in normal men. Thromb Haemostas 1984; 51: 110-4
26 Verheijen JH, Mullaart G, Chang ZG T, Kluft C, Wijngaards G. A simple, sensitive spectrophotometric assay for extrinsic (tissue-type) plasminogen activator applicable to measurements in plasma. Thromb Haemostas 1982; 48: 266-9
27 Ingerslev J. A sensitive ELISA for von Willebrand factui (vWF: Ag). Scand J Clin Lab Invest 1987; 47: 143-9
28 Reidy MA, Chopek M, Chao S, McDonald T, Schwartz SM. Injury induces increase of von Willebrand factor in rat endothelial cells. Am J Pathol 1989; 134: 857-64
29 Suffredini AF, Harpel PC, Parrillo JE. Promotion and subsequent inhibition of plasminogen activation after administration of intravenous endotoxin to normal subjects. N Engl J Med 1989; 320: 1165-72
30 Gralnick HR, McKeown LP, Wilson OM, Williams SB, Elin RJ. Von Willebrand factor release induced by endotoxin. J Lab Clin Med 1989; 113: 118-22
31 Novotny MJ, Turrentine MA, Johnson GS, Adams HR. Experimental endotoxemia increases plasma von Willebrand factor antigen concentrations in dogs with and without free-radical scavenger therapy. Circ Shock 1987; 23: 205-13
32 Tranquille N, Emeis JJ. Protein synthesis inhibition by cycloheximide does not affect the acute release of tissue-type plasminogen activator. Thromb Haemostas 1989; 61: 442-7
33 Nilsson IM, Holmberg L, Aberg M, Vilhardt H. The release of plasminogen activator and factor VIII after injection of DDAVP in healthy volunteers and in patients with von Willebrand’s disease. Scand J Haematol 1980; 24: 351-9
34 Ludlam CA, Peake IR, Allen N, Davies BL, Furlong RA, Bloom AL. Factor VIII and fibrinolytic response to desamino-8-D-arginine vasopressine in normal subjects and dissociate response in some patients with haemophilia and von Willebrand’s disease. Br J Haematol 1980; 45: 499-511
35 Korninger C, Niessner M, Lechner K. Impaired fibrinolytic response, to DDAVP and venous occlusion in a sub-group of patients with von Willebrand’s disease. Thromb Res 1981; 23: 365-74
36 Holmberg L, Nilsson IM, Wallen P, Astedt B. Studies on the blood plasminogen activator induced by l-desamino-8-D-arginine vasopressin with observations in von Willebrand’s disease. Proc Soc Exp Biol Med 1982; 170: 126-32
37 Jeanneau CH, Bachouchi NO, Gorin I, Meimon G, Sultan Y. Absence of functional activity of tissue plasminogen activator in patients with severe forms of von Willebrand’s disease. Br J Haematol 1987; 67: 79-88