Proteolytic and Immunologic Comparison of Human and Bovine von Willebrand Factor

Summary

The structures of bovine and human vWF were compared by proteolysis with Staphylococcus aureus V8 protease and rattlesnake venom Protease I. Fragments were analyzed for chain composition, heparin binding, collagen binding, platelet agglutinating activity and recognition by a panel of monoclonal antibodies which reacted with both bovine and human vWF. Similar large fragments from the C-terminal domain of vWF were seen in each case. The N-terminal domain resulting from cleavage of bovine vWF was much smaller than that seen upon digestion of human vWF with V8 protease. Protease I destroyed the heparin binding domain in human vWF. Bovine vWF was much less sensitive to proteolysis than was human vWF.

• References

• 1 Meyer D, Baumgartner HR. Role of von Willebrand factor in platelet adhesion to the subendothelium. Br J Haematol 1983; 54: 1-9
• 2 Titani K, Kumar S, Takio K, Ericsson LH, Wade RD, Ashida K, Walsh KA, Chopek MW, Sadler JE, Fujikawa K. Amino acid sequence of human von Willebrand factor. Biochemistry 1986; 25: 3171-84
• 3 Girma JP, Kalafatis M, Pietu G, Lavergne JM, Edgington TS, Meyer D. Mapping of distinct von Willebrand factor domains interacting with platelet GPIb and GPIIb/IIIa and with collagen using monoclonal antibodies. Blood 1986; 67: 1356-66
• 4 Fowler WE, Fretto LJ, Hamilton KK, Erickson HP, McKee PA. Substructure of human von Willebrand factor. J Clin Invest 1985; 76: 1491-500
• 5 Santoro SA. Adsorption of von Willebrand factor/factor VIII by the genetically distinct interstitial collagens. Thromb Res 1981; 21: 689-91
• 6 Fauvel F, Grant ME, Legrand YJ, Souchon H, Tobelem G, Jackson DS, Caen JP. Interaction of blood platelets with a microfibrillar extract from adult bovine aorta: requirement for von Willebrand factor. Proc Natl Acad Sci USA 1983; 80: 551-4
• 7 Mascelli MA, Kirby EP. Proteolytic studies on the structure of bovine von Willebrand Factor. Biochemistry 1988; 27: 1274-84
• 8 Bockenstedt P, Greenberg JM, Handin RI. Structural basis of von Willebrand factor binding to platelet glycoprotein Ib and collagen. Effects of disulfide reduction and limited proteolysis of polymeric von Willebrand factor. J Clin Invest 1986 77. 743-9
• 9 Girma JP, Chopek MW, Titani K, Davie EW. Limited proteolysis of human von Willebrand factor by Staphylococcus aureus V-8 protease: isolation and partial characterization of a platelet-binding domain. Biochemistry 1986; 25: 3156-63
• 10 Ohmori K, Fretto LJ, Harrison RL, Switzer ME, Erickson HP, McKee PA. Electron microscopy of human factor VII/von Willebrand glycoprotein. Effect of reducing reagents on structure and functions. J Cell Biol 1982 95. 632-40
• 11 Fujimura Y, Titani K, Holland LZ, Russel SR, Roberts JR, Elder JH, Ruggeri ZM, Zimmerman TS, Von Willebrand Factor. A reduced and alkylated 52/48 kDa fragment beginning at amino acid residue 449 contains the domain interacting with platelet glycoprotein Ib. J Biol Chem 1986; 261: 381-5
• 12 Fujimura Y, Titani K, Holland LZ, Roberts JR, Kostel P, Ruggeri ZM, Zimmerman TS. A heparin-binding domain of human von Willebrand factor. Characterization and localization to a tryptic fragment extending from amino acid residue val-449 to lys-728. J Biol Chem 1987 262. 1734-9
• 13 Roth GJ, Titani K, Hoyer LW, Hickey J. Localization of binding sites within human von Willebrand factor for monomeric Type III collagen. Biochemistry 1986; 25: 8357-61
• 14 Brinkhous KM, Thomas BD, Ibrahim SA, Read MS. Plasma levels of platelet aggregating factor/von Willebrand factor in various species. Thromb Res 1977; 11: 345-55
• 15 Furlan M, Stieger J, Beck EA. Exposure of platelet binding sites in von Willebrand factor by adsorption onto polystyrene latex particles. Biochim Biophys Acta 1987; 924: 27-37
• 16 Legrand YJ, Fauvel F, Gutman N, Muh JP, Tobelem G, Souchon H, Karniguian A, Caen JP. Microfibrils (MF) platelet interaction: requirement of von Willebrand factor. Thromb Res 1980; 19: 737-9
• 17 Pandya BV, Budzynski AZ. Anticoagulant protease from western diamondback rattlesnake (Crotalus atrox) venom. Biochemistry 1984; 23: 460-70
• 18 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-5
• 19 Fairbanks G, Steck TL, Wallach DF H. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 1971; 10: 2606-17
• 20 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem 1951; 193: 265
• 21 Mascelli MA, Edgington TS, Kirby EP. Characterization of a fragment of bovine von Willebrand factor that binds to platelets. Biochemistry 1986; 25: 6325-35
• 22 Newman J, Johnson AJ, Karpatkin MH, Puszkin S. Methods for the production of clinically effective intermediate and high-purity factor VIII concentrates. Br J Haematol 1971; 21: 1-19
• 23 Kirby EP. The agglutination of human platelets by bovine factor VIII : R. J Lab Clin Med 1982; 100: 963-76
• 24 Goodall AH, Meyer D. Registry of monoclonal antibodies to factor VIII and von Willebrand factor. Thromb Haemostas 1985; 54: 878-91
• 25 Meyer D, Zimmerman TS, Obert B, Edgington TS. Hybridoma antibodies to human von Willebrand factor. I. Characterization of seven clones. Br J Haematol 1984 57. 597-608
• 26 Legaz ME, Schmer G, Counts RB, Davie EW. Isolation and characterization of human factor VIII (Antihemophilic Factor). J Biol Chem 1973; 248: 3946-55