Expression of the Drug-Dependent Antigen for Quinine-Dependent Antiplatelet Antibodies on GP III a but not that on GPI b, II b or IX on Human Endothelial Cells

Sharron L Pfueller; Robyn A Bilston; Stephen Jane; Jannine Gibson

doi:10.1055/s-0038-1645209

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1990; 63(02): 279-281
DOI: 10.1055/s-0038-1645209

Original Article

Schattauer GmbH Stuttgart

Expression of the Drug-Dependent Antigen for Quinine-Dependent Antiplatelet Antibodies on GP III a but not that on GPI b, II b or IX on Human Endothelial Cells

Sharron L Pfueller

The Department of Medicine, Monash University Medical School, Alfred Hospital, Prahran, Victoria, Australia

,

Robyn A Bilston

The Department of Medicine, Monash University Medical School, Alfred Hospital, Prahran, Victoria, Australia

,

Stephen Jane

The Department of Medicine, Monash University Medical School, Alfred Hospital, Prahran, Victoria, Australia

,

Jannine Gibson

The Department of Medicine, Monash University Medical School, Alfred Hospital, Prahran, Victoria, Australia

› Author Affiliations

Abstract

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Summary

In quinine- and quinidine-induced thrombocytopenic purpura IgG antibodies are known to react in a drug-dependent manner with different combinations of surface glycoproteins (GP) Ib , IIb, IIIa and IX. Because endothelial cells share a number of properties of platelets, including the presence of GP Ilia and GPIb-like proteins, we have compared these two cell types for their quinine-dependent IgG binding abilities. By immunoblotting of endothelial cells, quinine-dependent IgG binding from four patient sera was observed only to a 93 kDa component corresponding to GP Ilia. Strong IgG binding independent of the drug was found at 170–180 kDa. Thus endothelial cells express the GP Ilia quinine-dependent epitope on platelet GP IIIa , but not those on other platelet glycoproteins.

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References

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