Luminographic Detection of von Willebrand Factor Multimers in Agarose Gels and on Nitrocellulose Membranes

U Budde; R Schneppenheim; H Plendl; J Dent; Z M Ruggeri; T S Zimmerman

doi:10.1055/s-0038-1645215

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1990; 63(02): 312-315
DOI: 10.1055/s-0038-1645215

Original Article

Schattauer GmbH Stuttgart

Luminographic Detection of von Willebrand Factor Multimers in Agarose Gels and on Nitrocellulose Membranes

Authors

U Budde

¹The Blood Transfusion Service AK Harburg, Hamburg, FRG
R Schneppenheim

²The Dept. of Pediatrics, University Clinic, Kiel, FRG
H Plendl

³The Dept. of Human Genetics, University Clinic, Kiel, FRG
J Dent

⁴The Scripps Clinic and Research Foundation, La Jolla, CA, USA
Z M Ruggeri

⁴The Scripps Clinic and Research Foundation, La Jolla, CA, USA
T S Zimmerman

⁴The Scripps Clinic and Research Foundation, La Jolla, CA, USA

Abstract

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Summary

Two methods for visualization of vWf multimers were compared with respect to sensitivity and detection of normal vWf and vWd variants IIA, IIB, IIC, IID, HE, and HE Autoradiography and luminography after electrotransfer of vWf multimers onto nitrocellulose showed comparable sensitivity with vWf: Ag detectable after 1:500 dilution of normal plasma. The least sensitive method was luminography in agarose gels with vWf: Ag detectable after 1:300dilution of normal plasma. No difference existed in the banding patterns of plasmas from patients with variant vWd.

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References

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