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Thromb Haemost 1987; 58(02): 682-685
DOI: 10.1055/s-0038-1645955
Original Article
Schattauer GmbH Stuttgart

Free Platelet Count and Size Distribution During C1q Inhibition of Collagen-Induced Platelet Aggregation

Gyorgy Csako
1   The Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, Md., USA
,
Eva A Suba
2   The Naval Medical Research Institute, Bethesda, Md., USA
› Author Affiliations
Further Information

Publication History

Received 29 September 1986

Accepted after revision 17 March 1987

Publication Date:
27 June 2018 (online)

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Summary

Electronic free platelet counting was more sensitive than turbidimetry to detect collagen-induced platelet activation in human platelet-rich plasma. Purified human Clq exhibited a greater inhibitory effect on collagen-induced platelet aggregation in turbidimetry than free platelet counting. Because the change from small to large platelet aggregates is responsible for the continuing increase in light transmission, Clq was likely more capable of blocking the formation of large platelet aggregates than the formation of small aggregates from single platelets. The iattr uf change by cullagcn in light tiansmissiun and fiec platelet count was reduced in the presence of Clq but the timing of the peak response remained the same. Electronic platelet sizing revealed that the volume of single platelets transiently increased during the turbidimetric “lag phase”. The mean, mode and median volume of the remaining free platelets then decreased, suggesting a selective loss of large, functionally more active platelets and/or platelet degranulation. Clq had no effect on the volume increment during the “lag phase”, but reduced the subsequent fall in the volume of free platelets.

Key words

Collagen - Subcomponent Clq - Platelet aggregation - Free platelet count - Platelet sizing
 

  • References

  • 1 Porter RR, Reid K BM. The biochemistry of complement. Nature 1978; 275: 699-704
    CrossrefPubMedGoogle Scholar
  • 2 Hugues J. Accolement de plaquettes au collagen. C R Soc Biol 1960; 154: 866-868
    PubMedGoogle Scholar
  • 3 Suba EA, Csako G. Clq (Cl) receptor on human platelets: inhibition of collagen-induced platelet aggregation by Clq (Cl) molecules. J Immunol 1976; 117: 304-309
    PubMedGoogle Scholar
  • 4 Csákó G, Suba EA. A physiologic regulator of collagen-induced platelet aggregation. Inhibition by Clq. Thromb Haemostas 1981; 45: 110-115
    PubMedGoogle Scholar
  • 5 Csako G, Suba EA, Herp A. Similarities and dissimilarities between the binding ability of Clq and collagen. Clin Exp Immunol 1981; 44: 181-190
    PubMedGoogle Scholar
  • 6 Csako G, Suba EA, Herp A. Effect of collagen-like substances (Clq. acetylcholinesterase and elastin) on collagen-induced platelet aggregation. Haemostasis 1982; 11: 204-209
    PubMedGoogle Scholar
  • 7 Cazenave J-P, Assimeh SN, Painter RH, Packham MA, Mustard JE. Clq inhibition of the interaction of collagen with human platelets. J Immunol 1976; 116: 162-163
    PubMedGoogle Scholar
  • 8 Wautier J-L, Souchon H, Reid K BM, Peltier AP, Caen JP. Studies on the mode of reaction of the first component of complement with platelets: interaction between the collagen-like portion of Clq and platelets. Immunochemistry 1977; 14: 763-766
    CrossrefPubMedGoogle Scholar
  • 9 Mundschenk DD, Connelly DP, White JG, Brunning RD. An improved technique for the electronic measurement of platelet size and shape. J Lab Clin Med 1976; 88: 301-315
    PubMedGoogle Scholar
  • 10 Born G VR. Quantitative investigations into the aggregation of blood platelets. J Physiol (Lond) 1962; 162: 67P-8P
    PubMedGoogle Scholar
  • 11 Agnello V, Winchester RJ, Kunkel HG. Precipitin reactions of the Clq component of complement with aggregated y-globulin and immune complexes in gel diffusion. Immunology 1970; 19: 909-919
    PubMedGoogle Scholar
  • 12 McLean MR, Hause LL. Collagen-induced platelet aggregation as studied by electronic particle size analysis. Thromb Haemostas 1981; 46: 731-733
    PubMedGoogle Scholar
  • 13 Gear A RL. Rapid reactions of platelets studied by a quenched-flow approach: aggregation kinetics. J Lab Clin Med 1982; 100: 866-886
    PubMedGoogle Scholar
  • 14 Packham MA. Methods for detection of hypersensitive platelets. Thromb Haemostas 1978; 40: 175-195
    PubMedGoogle Scholar
  • 15 Manucci PM, Sharp AA. Platelet volume and shape in relation to aggregation and adhesion. Br J Haemat 1967; 13: 604-617
    CrossrefPubMedGoogle Scholar
  • 16 Paulus JM, Bury J, Grosdent JC. Control of platelet territory development in megakaryocytes. Blood Cells 1979; 5: 59-88
    PubMedGoogle Scholar
  • 17 Born G VR, Hume M. Effects of the numbers and sizes of platelet aggregates on the optical density of plasma. Nature 1967; 215: 1027-1029
    CrossrefPubMedGoogle Scholar
  • 18 Nichols AR, Bosmann HB. Platelet aggregation: newly quantified using nonempirical parameters. Thromb Haemostas 1979; 42: 679-693
    PubMedGoogle Scholar
  • 19 Frojmovic MM, Milton JG, Duchastel A. Microscopic measurements of platelet aggregation reveal a low ADP-deprendent process distinct from turbidimetrically measured aggregation. J Lab Clin Med 1983; 101: 964-976
    PubMedGoogle Scholar
  • 20 Milton JG, Frojmovic MM. Turbidimetric evaluations of platelet activation: Relative contributions of measured shape change, volume, and early aggregation. J Pharmacol Meth 1983; 9: 101-115
    CrossrefPubMedGoogle Scholar
  • 21 Cronberg S. Evaluation of platelet aggregation. Coagulation 1970; 3: 139-151
    PubMedGoogle Scholar
  • 22 Thompson NT, Scrutton MC, Wallis RB. Particle volume changes associated with light transmittance changes in the platelet aggregometer: dependence upon aggregating agent and effectiveness of stimulus. Thrombos Res 1986; 41: 615-626
    CrossrefPubMedGoogle Scholar
  • 23 Lumley P, Humphrey P PA. A method for quantitating platelet aggregation and analyzing drug-receptor interactions on platelets in whole blood in vitro. J Pharmacol Meth 1981; 6: 153-166
    CrossrefPubMedGoogle Scholar
  • 24 Fox SC, Burgess-Wilson M, Heptinstall S, Mitchell J RA. Platelet aggregation in whole blood determined using the Ultra-Flo 100 platelet counter. Thromb Haemostas 1982; 48: 327-329
    PubMedGoogle Scholar
  • 25 Csako G, Suba EA, Ahlgren A, Tsai C-M, Elin RJ. Relation of structure to function for the U.S. reference standard endotoxin after exposure to 60Co radiation. J Infect Dis 1986; 153: 98-108
    CrossrefPubMedGoogle Scholar
  • 26 Santoro SA, Cunningham LW. Collagen-mediated platelet aggregation: the role of multiple interactions between the platelet surface and collagen. Thromb Haemostas 1980; 43: 158-162
    PubMedGoogle Scholar
  • 27 Solis RT, Wright CB, Gibbs MB. Electronic particle size measurements of platelet aggregates formed in vitro. J Applied Physiol 1975; 38: 739-744
    CrossrefPubMedGoogle Scholar
  • 28 Karpatkin S. Heterogeneity of human platelets. II. Functional evidence suggestive of young and old platelets. J Clin Invest 1969; 48: 1083-1087
    CrossrefPubMedGoogle Scholar
  • 29 Corash L, Tan H, Gralnick HR. Heterogeneity of human whole blood platelet subpopulations. I. Relationship between buoyant density, cell volume and ultrastructure. Blood 1977; 49: 71-87
    PubMedGoogle Scholar
  • 30 Karpatkin S. Heterogeneity of human platelets. VI. Correlation of platelet function with platelet volume. Blood 1978; 51: 307-316
    PubMedGoogle Scholar
  • 31 Thompson CB, Eaton KA, Princiotta SM, Rushin CA. Valeri C R. Size dependent platelet subpopulations: relationship of platelet volume to ultrastructure, enzymatic activity, and function. Br J Haemat 1982; 50: 509-519
    CrossrefPubMedGoogle Scholar
  • 32 Morgenstern E, Kho A. Morphometrische Untersuchungen an Blutplattchen; Veranderungen der Plattchenstruktur bei Pseudopodienbildung und Aggregation. Cytobiologie 1977; 15: 233-249
    PubMedGoogle Scholar