Subscribe to RSS
DOI: 10.1055/s-0038-1645962
The Response of Megakaryocytes with Processes to Thrombin
Publication History
Received 15 July 1986
Accepted after revision 02 April 1987
Publication Date:
27 June 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198702/lookinside/thumbnails/10-1055-s-0038-1645962-1.jpg)
Summary
The response of megakaryocytes to thrombin (1-10 U/ml) has been examined by time-lapse cinemicrography and electron microscopy. The study was confined to mature megakaryocytes which had developed processes following incubation in vitro. The initial response of all cells was to undergo retraction of processes, behaviour thought to be linked with the depolymerization of microtubules which extend longitudinally through the processes. The majority of cells completely withdrew their processes, but about 30% responded differently and underwent only limited retraction, followed by secretion. Analysis of time-lapse film showed that processes from the latter group of cells had formed attachments with the coverslip prior to exposure to thrombin. Within the partially retracted processes of these cells, secretory granules were found to be clustered centripetally and enveloped by a rnicrofilamentous structure in the form of a cylinder. Vacuoles appeared, some of which were located outside the rnicrofilamentous structure. Microtubules were present, but many appeared disorientated. The shape of the rnicrofilamentous structure suggests that the cytoplasm is not organized into putative platelets at the time of process formation.
-
References
- 1 White JG, Gerrard JM. Interaction of microtubules and microfilaments in platelet contractile physiology. Meth Achiev exp Path 1979; 9: 1-39
- 2 Painter RG, Ginsberg MH. Centripetal myosin redistribution in thrombin-stimulated platelets. Exp Cell Res 1984; 155: 198-212
- 3 Stenberg PE, Shuman MA, Levine SP, Bainton DF. Redistribution of alpha-granules and their contents in thrombin-stimulated platelets. J Cell Biol 1984; 98: 748-760
- 4 Fedorko ME. The functional capacity of guinea-pig megakaryocytes. 1. Lab Invest 1977; 36: 310-320
- 5 Miller JL. Characterization of the megakaryocyte secretory response: studies of continuously monitored release of endogenous ATP. Blood 1983; 61: 967-972
- 6 Leven RM, Nachmias VT. Cultured megakaryocytes: changes in the cytoskele-ton after ADP-induced spreading. J Cell Biol 1982; 92: 313-323
- 7 Radley JM, Haller CJ. The demarcation membrane system of the megakaryocyte: a misnomer?. Blood 1982; 60: 213-219
- 8 Haller CJ, Radley JM. Time-lapse cinemicrography and scanning electron microscopy of platelet formation by megakaryocytes. Blood Cells 1983; 9: 407-418
- 9 Penington DG, Streatfield K, Roxburgh AE. Megakaryocytes and the heterogeneity of circulating platelets. Br J Haematol 1976; 34: 639-653
- 10 Radley JM, Scurfield G. Effects of 5-fluorouracil on mouse bone marrow. Br J Haematol 1979; 43: 341-351
- 11 Thiery JP, Bessis M. Mecamsme de la plaquettogenese. Rev Hematol 1956; 11: 162-174
- 12 Frojmovic MM, Milton JG. Human platelet size, shape, and related functions in health and disease. Physiol Rev 1982; 62: 185-261
- 13 Gear AR. Rapid platelet morphological changes visualized by scanning electron microscopy: kinetics derived from a quenched flow approach. Br J Haematol 1984; 56: 387-398
- 14 Deranleau DA, Dubler D, Rothen C, Luscher EF. Transient kinetics of the rapid shape change of unstirred human blood platelets stimulated with ADP. Proc Natl Acad Sci USA 1982; 79: 7297-7301
- 15 Hantgan RR. A study of the kinetics of ADP-triggered platelet shape change. Blood 1984; 64: 896-906
- 16 White JG. Fine structural alterations induced in platelets by adenosine diphosphate. Blood 1968; 31: 604-622
- 17 Steiner M, Ikeda Y. Quantitative assessment of polymerized and depolymerized platelet microtubules. Changes caused by aggregating agents. J Clin Invest 1979; 63: 443-448
- 18 Kenney DM, Chao FC. Ionophore-induced disassembly of blood platelet microtubules: effect of cyclic AMP and indomethacin. J Cell Physiol 1980; 103: 289-298
- 19 Mattson JC, Zuiches CA. Elucidation of the platelet cytoskeleton. Ann NY Acad Sci 1981; 370: 11-21
- 20 Lewis JC, White MS, Prater T, Taylor RG, Davis KS. Ultrastructural analysis of platelets in nonhuman primates. III. Stereo microscopy of microtubules during platelet adhesion and the release reaction. Exp Mol Pathol 1982; 37: 370-381
- 21 Debus E, Weber K, Osborn M. The cytoskeleton of blood platelets viewed by immunofluorescence microscopy. Eu J Cell Biol 1981; 24: 45-52
- 22 White JG, Sauk JJ. Microtubule coils in spread blood platelets. Blood 1984; 64: 470-478
- 23 White JG, Krumwiede M, Sauk JJ. Microtubule reassembly in surface-activated platelets. Blood 1985; 65: 1494-1503
- 24 White JG, Rao G HR. Influence of a microtubule stabilizing agent on platelet structural physiology. Am J Pathol 1983; 112: 207-217
- 25 Radley JM, Scurfield G. The mechanism of platelet release. Blood 1980; 56: 996-999
- 26 Yamada E. The fine structure of the megakaryocyte in the mouse spleen. Acta Anat (Basel) 1957; 29: 267-290
- 27 Becker RP, De Bruyn P PH. The transmural passage of blood cells into myeloid sinusoids and the entry of platelets into the sinusoidal circulation; a scanning electron microscopic investigation. Am J Anat 1976; 145: 183-206