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DOI: 10.1055/s-0038-1646014
Platelet Density-Dependent Partition of Platelet- von Willebrand Factor Between Alpha Granule and Non-Alpha Granule Pools
Publication History
Received 27 January 1987
Accepted after revision 11 June 1987
Publication Date:
28 June 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198703/lookinside/thumbnails/10-1055-s-0038-1646014-1.jpg)
Summary
In this study, we demonstrate that platelets contain a small hut significant amount of platelet-von Willebrand factor(vWf) not associated with α-granules. When platelets free of plasma proteins are exposed to micromolar concentrations ofdigitonin, plasma membrane permeabilization occurs without disruption of platelet granules. Employing this technique, wehave found that upon exposure of a total platelet population to 8 μM digitonin, 5% of total platelet-vWf is released into the supernatant; this occurs without release of (β-TG from α-granules. When platelets of discrete buoyant density profiles are tested, this extragranular platelet-vWf increased with decreasing platelet density. These findings suggest that a redistribution of platelet-vWf from α-granule to non-granule sites occurs coincident with a decrease inplatelet buoyant density.
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References
- 1 Slot JW, Bouma BN, Montgomery R, Zimmerman TS. Platelet Factor VUI-related Antigen: Immunofluoresccnt Localization. Thromb Res 1978; 13: 871-881
- 2 Zucker MB, Brockman MJ, Kaplan KL. Factor VIII-related Antigen in Platelets. Localization and Release by Thrombin and Collagen. J Lab Clin Med 1979; 94: 675-682
- 3 Wencel-Drake JD, Painter RG, Zimmerman TS, Ginsberg MH. Ultrastructural Localization of Human Platelet Thrombospondin, Fibrinogen, Fibronectin and von Willebrand Factor in Frozen Thin Section. Blood 1985; 65: 929-938
- 4 Howard MA, Montgomery DC, Hardisty RM. Factor Vlll-related Antigen in Platelets. Thromb Res 1974; 4: 617-624
- 5 Corash L, Mok Y. Platelet Heterogeneity: Density Distribution of Alpha Granule and Lysosomal Constituents in Native and Thrombin Stimulated Platelet Density Cohorts. Blood 1984; 64 (05) Suppl 1): 245 a, Abstr 890
- 6 Corash L, Costa JL, Shafer B, Donlon JA, Murphy D. Heterogeneity of Human Whole Blood Platelet Subpopulations III. Density-Dependent Differences in Subcellular Constituents. Blood 1984; 64: 185-193
- 7 Zuurendonck PF, Tager FM. Rapid Separation of Particulate Components and Soluble Cytoplasm of Isolated Rat-liver Cells. Biochim Biophys Acta 1974; 333: 393-399
- 8 Akkerman J WN, Ebberink R HM, Lips J PM, Christiaens G CM L. Rapid Separation of Cytosol and Particle Fraction of Human Platelets by Digitonin-induced Cell Damage. Br. J. Haem. 1980; 44: 291-300
- 9 Parker RI, Rick ME, Gralnick HR. A Method to Minimize Platelet Activation during Platelet Isolation. Thromb Res 1984; 36: 265-270
- 10 Corash L, Tan H, Gralnick HR. Heterogeneity of Human Whole Blood Platelet Subpopulations. I. Relationship Between Buoyant Density, Cell Volume, and Ultrastructure. Blood 1977; 49: 71-87
- 11 Parker RI, Rick ME, Gralnick HR. The Effect of Calcium on the Availability of Platelet von Willebrand Factor. J Lab Clin Med 1985; 106: 336-342
- 12 Hoyer LW. Immunologic Studies of Antihemophilic Factor (AHF, Factor VIII). IV. Radioimmunoassay ot AHF Antigen. J Lab Clin Med 1972; 80: 922-933
- 13 Hoyer LW, Shainoff JR. Factor-Vlll-related Protein Circulates in Normal Human Plasma as High Molecular Weight Multimers. Blood 1980; 55: 1056-1059
- 14 Parker RI, Rick ME, Gralnick HR. Absence of the Largest von Willebrand Factor Multimers in the Platelets of a Patient with Lactoferrin Deficiency and a Bleeding Tendency. Clin Res 1984; 32 (02) 318 A
- 15 Gralnick HR, Rick MR, McKeown LP, Williams SB, Parker RI, Maisonneuve P, Jeanneau C, Sultan Y. Platelet, von Willebrand Factor: An Important Determinant of the Bleeding in Type I von Willebrand’s Disease. Blood 1986; 68: 58-61
- 16 Jeanneau C, Sultan Y. Immunocytochemical Localization of Factor VUI/von Willebrand Factor Antigen in Human Platelets. Biol Cell 1983; 49: 237-242
- 17 Wencel-Drake JD, Plow EF, Zimmerman TS, Painter RG, Ginsberg MH. Immunolocalization of Adhesive Glycoproteins in Resting and Thrombin-Stimulated Platelets. Am J Pathol 1984; 115: 156-164
- 18 Stenberg PE, Shuman MA, Levine SP, Bainton DF. Redistribution of Alpha-Granules and their Contents in Thrombin-Stimulated Platelets. J Cell Biol 1984; 98: 748-760
- 19 Parker RI, Gralnick HR. Identification of Platelet Glycoprotein IIb/IIIa as the Major Binding Site for Released Platelet von Willebrand Factor. Blood 1986; 68: 732-736