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DOI: 10.1055/s-0038-1646017
The Effect of the Intracellular Calcium Chelator Quin-2 on the Platelet Phosphoinositide Metabolism, Protein Phosphorylation and Morphology
Publication History
Received 13 March 1987
Accepted after revision 19 June 1987
Publication Date:
28 June 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198703/lookinside/thumbnails/10-1055-s-0038-1646017-1.jpg)
Summary
When human platelets prelabeled with [32P] orthophosphate were loaded with Quin-2, the 32P-incorporation in phosphatidic acid, phosphatidylinositol-4 phosphate and phosphatidylinositol-4,5 bisphosphate increased, that in phosphatidylinositol decreased. These effects occurred in a Quin-2-concentration- dependent manner. On stimulation of the serotonin-S2 receptor, signal transduction, measured as changes in labeling in phospholipids and phosphoproteins, was altered in the presence of the fluorophore. Microscopic evaluation illustrated that Quin-2 affected platelet morphology as well in resting as in stimulated platelets. A correlation between platelet shape change and myosin light chain phosphorylation was apparent.
The data evidence that the Quin-2 that is widely used for fluorometric determination of intracellular Ca2+, affects the metabolism of inositol-containing phospholipids whose breakdown is a key event in Ca2i+-mobilization on excitatory platelet activation. These fluorophore-induced alterations might, besides the Ca2-chelating properties, play an important role in the Ca2+ dependent signalling processes in these cells.
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