Download PDF
Thromb Haemost 1987; 58(03): 866-871
DOI: 10.1055/s-0038-1646022
Original Article
Schattauer GmbH Stuttgart

Redistribution of Platelet Glycoproteins Induced by Allo- and Autoantibodies

S Santoso
1   The Institute of Clinical Immunology and Blood Transfusion, Justus-Liebig-University, Gießen, FRG
,
V Kiefel
1   The Institute of Clinical Immunology and Blood Transfusion, Justus-Liebig-University, Gießen, FRG
,
C Mueller-Eckhardt
1   The Institute of Clinical Immunology and Blood Transfusion, Justus-Liebig-University, Gießen, FRG
› Author Affiliations
Further Information

Publication History

Received 20 February 1987

Accepted after revision 02 June 1987

Publication Date:
28 June 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

The redistribution of platelet glycoproteins (GP) Ib, IIb and IIIa in response to stimulation with human platelet specific alloantibodies, autoantibodies and a quinidine-dependent antibody was investigated using immunofluorescence and a quantitative radioimmunoassay. The platelet GPs carrying the corresponding epitopes were determined using immunoblot technique or radioimmunoprecipitation. In accordance with previous results obtained with murine monoclonal platelet specific antibodies, redistribution upon stimulation with human platelet reactive antibodies was observed only due to reactions with epitopes on GP IIb, IIIa, respectively, but not on GP Ib. We therefore believe that membrane redistribution of human platelets induced by various antibodies is a function of the GP recognized by those antibodies rather than the source of the platelet reactive antibody.

Keywords

Platelet glycoprotein - Platelet specific antibodies - Redistribution - Endocytosis
 

  • References

  • 1 George JN, Nurden AT, Phillips DR. Molecular defects in interaction of platelets with the vessel wall. N Engl J Med 1984; 311: 1084-1098
    CrossrefPubMedGoogle Scholar
  • 2 Kornecki E, Chung SY, Holt JC, Cierniewski CS, Tuszynski GP, Niewiarowski S. Identification of P1A1 alloantigen domain on a 66 kDa protein derived from glycoprotein Ilia of human platelets. Biochim Biophys Acta 1985; 818: 285-290
    CrossrefPubMedGoogle Scholar
  • 3 Kunicki TJ, Aster RH. Isolation and immunologic characterization of the human platelet alloantigen, P1A1 . Mol Immunol 1979; 16: 353-360
    CrossrefPubMedGoogle Scholar
  • 4 Borne von dem Kr A EG, Riesz von E, Verheugt F WA, Cate ten JW, Koppe JG, Engelfriet CP, Nijenhuis LE. Baka, a new platelet-specific antigen involved in neonatal allo-immune thrombocytopenia. Vox Sang 1980; 39: 113-120
    PubMedGoogle Scholar
  • 5 Boizard B, Wautier JL. Leka, a new platelet antigen absent in Glanzmann’s thrombasthenia. Vox Sang 1984; 46: 47-54
    PubMedGoogle Scholar
  • 6 Mulder A, Leeuwen van EF, Veenboer G JM, Tetteroo P AT, Borne von dem Kr A EG. Immunochemical characterization of platelet-specific alloantigens. Scand J Haematol 1984; 33: 267-274
    PubMedGoogle Scholar
  • 7 Kieffer N, Boizard B, Didry D, Wautier J-L, Nurden AT. Immunochemical characterization of the platelet-specific alloantigen Leka. A comparative study with the P1A1 alloantigen. Blood 1984; 64: 1212-1219
    PubMedGoogle Scholar
  • 8 Kunicki TJ, Christie DJ, Aster RH. The human platelet receptor(s) for quinine/quinidine-dependent antibodies. Blood Cells 1983; 9: 293-301
    PubMedGoogle Scholar
  • 9 Berndt MC, Chong BH, Bull HA, Zola H, Castaldi PA. Molecular characterization of quinine/quinidine drug-dependent antibody platelet interaction using monoclonal antibodies. Blood 1985; 66: 1292-1301
    PubMedGoogle Scholar
  • 10 Woods Jr VL, Oh EH, Mason D, McMillan R. Autoantibodies against the platelet glycoprotein IIb/IIIa complex in patients with chronic ITP. Blood 1984; 63: 368-375
    PubMedGoogle Scholar
  • 11 Woods Jr VL, Kurata Y, Montgomery RR, Tani P, Mason D, Oh EH, McMillan R. Autoantibodies against platelet glycoprotein Ib in patients with chronic immune thrombocytopenic purpura. Blood 1984; 64: 156-160
    PubMedGoogle Scholar
  • 12 Szatkowski NS, Kunicki TJ, Aster RH. Identification of glycoprotein Ib as a target for autoantibody in idiopathic (autoimmune) thrombocytopenic purpura. Blood 1986; 67: 310-315
    PubMedGoogle Scholar
  • 13 Cines DB, Schreiber AD. Effect of anti-PlA1 antibody on human platelets. I. The role of complement. Blood 1979; 53: 567-577
    PubMedGoogle Scholar
  • 14 Schreiber AD, Cines DB, Zmijewski C, Colman RW. Effect of anti-PC1 antibody on human platelets. II. Mechanism of the complement-dependent release reaction. Blood 1979; 53: 578-587
    PubMedGoogle Scholar
  • 15 Leeuwen van EF, Leeksma OC, Mourik van JA, Engelfriet CP, Borne von dem Kr A EG. Effect of the binding of anti-Zwa antibodies on platelet function. Vox Sang 1984; 47: 280-289
    PubMedGoogle Scholar
  • 16 Saunders P WG, Durack BE, Narang HK. Zwa antigen distribution on the human platelet: an electron microscope study using a colloidal gold labelled marker. Br J Haematol 1985; 59: 209-219
    CrossrefPubMedGoogle Scholar
  • 17 Saunders P WG, Durack BE, Narang HK. Observations on the distribution of the Zwa (P1A1) antigen on fixed and unfixed platelets. Br J Haematol 1986; 62: 631-634
    CrossrefPubMedGoogle Scholar
  • 18 Santoso S, Zimmermann U, Neppert J, Mueller-Eckhardt C. Receptor patching and capping of platelet membranes induced by monoclonal antibodies. Blood 1986; 67: 343-349
    PubMedGoogle Scholar
  • 19 Heinrich D, Santoso S, Scharf T, Weinel R, Mueller-Eckhardt C. Monoclonal platelet antibodies with Zw(a) specificity have different inhibitory activity on platelet function. In: Monoclonal antibodies and human blood platelets. McGregor JL. (ed) Elsevier Science Publishers; Amsterdam, New York, Oxford: 1986. pp 167-177
    Google Scholar
  • 20 Schneider W, Schnaidt M. The platelet adhesion immunofluorescence test: a modification of the platelet suspension immunofluorescence test. Blut 1981; 43: 389-392
    CrossrefPubMedGoogle Scholar
  • 21 Kiefel V, Santoso S, Schmidt S, Salama A, Mueller-Eckhardt C. Metabolite-specific (IgG) and drug-specific antibodies (IgG, IgM) in two cases of trimethoprim-sulfamethoxazole-induced immune thrombocytopenia. Transfusion 1987; 27: 262-265
    CrossrefPubMedGoogle Scholar
  • 22 McFarlane AS. Efficient trace-labelling of proteins with iodine. Nature 1958; 182: 53
    PubMedGoogle Scholar
  • 23 Zola H, McNamara PJ, Beckman I GR, Brooks DA, Macardle PJ, Bradley J, Goodall K, Skinner J, Collins R, Berndt MC. Monoclonal antibodies against antigens of the human platelet surface: preparation and properties. Pathology 1984; 16: 73-78
    CrossrefPubMedGoogle Scholar
  • 24 Santoso S, Lohmeyer J, Rennich H, Clemetson KJ, Mueller-Eckhardt C. Platelet surface antigens: Analysis by monoclonal antibodies. Blut 1984; 48: 161-170
    CrossrefPubMedGoogle Scholar
  • 25 Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 1979; 76: 4350-4354
    CrossrefPubMedGoogle Scholar
  • 26 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T. Nature 1970; 227: 680
    CrossrefPubMedGoogle Scholar
  • 27 Steiner B, Clemetson KJ, Liischer EF. Improvement of the periodate-borhydride surface-labeling method for human blood platelets. Thromb Res 1983; 29: 43-52
    CrossrefPubMedGoogle Scholar
  • 28 Rosa J-P, Kieffer N, Didry D, Pidard D, Kunicki TJ, Nurden AT. The human platelet membrane glycoprotein complex GP IIb-IIIa expresses antigenic sites not exposed on the dissociated glycoproteins. Blood 1984; 64: 1246-1253
    PubMedGoogle Scholar
  • 29 Coller BS, Peerschke EI, Scudder LE, Sullivan CA. Studies with a murine monoclonal antibody that abolishes ristocetin-induced binding of von Willebrand factor to platelets: Additional evidence in support of GP Ib as a platelet receptor for von Willebrand factor. Blood 1983; 61: 99
    PubMedGoogle Scholar
  • 30 Phillips DR, Jennings LK, Edwards HH. Identification of membrane proteins mediating the interaction of human platelets. J Cell Biol 1980; 86: 77-86
    CrossrefPubMedGoogle Scholar
  • 31 Wheeler ME, Cox AC, Carroll RC. Retention of the glycoprotein IIb-IIIa complex in the isolated platelet cytoskeleton. Effects of separable assembly of platelet pseudopodal and contractile cytoskele-tons. J Clin Invest 1984; 74: 1080-1089
    CrossrefPubMedGoogle Scholar
  • 32 Fox J EB, Reynolds CC, Phillips DR. Calcium-dependent proteolysis occurs during platelet aggregation. J Biol Chem 1983; 258: 9973-9981
    PubMedGoogle Scholar
  • 33 Fox J EB. Linkage of a membrane skeleton to integral membrane glycoproteins in human platelets. Identification of one of the glycoproteins as glycoprotein Ib. J Clin Invest 1985; 76: 1673-1683
    CrossrefPubMedGoogle Scholar
  • 34 Asch AS, Leung L LK, Polley MJ, Nachman RL. Platelet membrane topography: Colocalization of thrombospondin and fibrinogen with the glycoprotein IIb-IIIa complex. Blood 1985; 66: 926-934
    PubMedGoogle Scholar