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Thromb Haemost 1987; 58(03): 866-871
DOI: 10.1055/s-0038-1646022
Original Article
Schattauer GmbH Stuttgart

Redistribution of Platelet Glycoproteins Induced by Allo- and Autoantibodies

S Santoso
1   The Institute of Clinical Immunology and Blood Transfusion, Justus-Liebig-University, Gießen, FRG
,
V Kiefel
1   The Institute of Clinical Immunology and Blood Transfusion, Justus-Liebig-University, Gießen, FRG
,
C Mueller-Eckhardt
1   The Institute of Clinical Immunology and Blood Transfusion, Justus-Liebig-University, Gießen, FRG
› Institutsangaben
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Publikationsverlauf

Received 20. Februar 1987

Accepted after revision 02. Juni 1987

Publikationsdatum:
28. Juni 2018 (online)

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Summary

The redistribution of platelet glycoproteins (GP) Ib, IIb and IIIa in response to stimulation with human platelet specific alloantibodies, autoantibodies and a quinidine-dependent antibody was investigated using immunofluorescence and a quantitative radioimmunoassay. The platelet GPs carrying the corresponding epitopes were determined using immunoblot technique or radioimmunoprecipitation. In accordance with previous results obtained with murine monoclonal platelet specific antibodies, redistribution upon stimulation with human platelet reactive antibodies was observed only due to reactions with epitopes on GP IIb, IIIa, respectively, but not on GP Ib. We therefore believe that membrane redistribution of human platelets induced by various antibodies is a function of the GP recognized by those antibodies rather than the source of the platelet reactive antibody.

Keywords

Platelet glycoprotein - Platelet specific antibodies - Redistribution - Endocytosis
 

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