Two Genes Homologous with Human Protein S cDNA Are Located on Chromosome 3

J K Ploos van Amstel; A L van der Zander; E Bakker; P H Reitsma; R M Bertina

doi:10.1055/s-0038-1646041

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1987; 58(04): 982-987
DOI: 10.1055/s-0038-1646041

Original Article

Schattauer GmbH Stuttgart

Two Genes Homologous with Human Protein S cDNA Are Located on Chromosome 3

J K Ploos van Amstel

The Hemostasis and Thrombosis Research Unit, Department of Hematology, Leiden University Hospital, Leiden, The Netherlands

,

A L van der Zander

The Hemostasis and Thrombosis Research Unit, Department of Hematology, Leiden University Hospital, Leiden, The Netherlands

,

E Bakker

¹Department of Human Genetics, Leiden University Hospital, Leiden, The Netherlands

,

P H Reitsma

The Hemostasis and Thrombosis Research Unit, Department of Hematology, Leiden University Hospital, Leiden, The Netherlands

,

R M Bertina

The Hemostasis and Thrombosis Research Unit, Department of Hematology, Leiden University Hospital, Leiden, The Netherlands

› Author Affiliations

Abstract

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Summary

A cDNA coding for the carboxy-terminal region of human protein S and containing a complete 3’-untranslated region, was isolated by a combination of antibody screening of a λgt11 human liver cDNA expression library and in situ hybridization of a pUC9 human liver cDNA library.

Hybridization analysis of human total DNA with radiolabelled non-overlapping cDNA restriction fragments revealed the existence of two genes per haploid genome homologous with the protein S cDNA. Both genes were mapped to chromosome 3 using human-rodent cell hybrids. Neither of the genes showed polymorphism for sixteen different enzymes upon hybridization with the protein S cDNA.

Keywords

Protein S - Gene - Chromosome - RFLP - Thrombosis

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References

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