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Thromb Haemost 1987; 58(04): 982-987
DOI: 10.1055/s-0038-1646041
Original Article
Schattauer GmbH Stuttgart

Two Genes Homologous with Human Protein S cDNA Are Located on Chromosome 3

J K Ploos van Amstel
The Hemostasis and Thrombosis Research Unit, Department of Hematology, Leiden University Hospital, Leiden, The Netherlands
,
A L van der Zander
The Hemostasis and Thrombosis Research Unit, Department of Hematology, Leiden University Hospital, Leiden, The Netherlands
,
E Bakker
1   Department of Human Genetics, Leiden University Hospital, Leiden, The Netherlands
,
P H Reitsma
The Hemostasis and Thrombosis Research Unit, Department of Hematology, Leiden University Hospital, Leiden, The Netherlands
,
R M Bertina
The Hemostasis and Thrombosis Research Unit, Department of Hematology, Leiden University Hospital, Leiden, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 18 March 1987

Accepted after revision 17 July 1987

Publication Date:
29 June 2018 (online)

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Summary

A cDNA coding for the carboxy-terminal region of human protein S and containing a complete 3’-untranslated region, was isolated by a combination of antibody screening of a λgt11 human liver cDNA expression library and in situ hybridization of a pUC9 human liver cDNA library.

Hybridization analysis of human total DNA with radiolabelled non-overlapping cDNA restriction fragments revealed the existence of two genes per haploid genome homologous with the protein S cDNA. Both genes were mapped to chromosome 3 using human-rodent cell hybrids. Neither of the genes showed polymorphism for sixteen different enzymes upon hybridization with the protein S cDNA.

Keywords

Protein S - Gene - Chromosome - RFLP - Thrombosis
 

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