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Thromb Haemost 1978; 39(01): 001-011
DOI: 10.1055/s-0038-1646118
Original Article
Schattauer GmbH Stuttgart

Radioimmunoassay of an Early Plasmin Degradation Product of Human Fibrinogen, “Fragment A”, and its Clinical Application

Kimiteru Takagi
The Department of Clinical Pathology, Jichi Medical School, 3311—1, Yakushiji, Minamikawachmachi, Kawachigun, Tochigiken, Japan
,
Tadashi Kawai
The Department of Clinical Pathology, Jichi Medical School, 3311—1, Yakushiji, Minamikawachmachi, Kawachigun, Tochigiken, Japan
› Author Affiliations
Further Information

Publication History

Received 25 May 1997

Accepted 25 June 1997

Publication Date:
12 July 2018 (online)

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Summary

Upon the plasmin digestion of human fibrinogen, an early cleavage product, which has been designated as fragment A, was isolated, and to study the action of plasmin in the circulation, radioimmunoassay for fragment A was carried out. This assay used rabbit immune serum obtained by injection of fragment A mixed with complete Freund’s adjuvant, and fragment A was labeled with 125I using the Chloramin-T method.

In 20 normal healthy donors its serum level was 3.57 ± 1.62 μg/ml (mean ± SD), and it was increased significantly in certain diseases, such as acute leukemias, cardiovascular disorders, malignancies, renal failure, systemic lupus erythematosus and sepsis.

 

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