A Rapid Turbidimetric Determination of Fibrinogen Degradation Products (FDP)

Summary

A rapid and simple turbidimetric determination of fibrinogen degradation products is described. This method is based on the increase of the turbidity due to the formation of precipitating antigen-antibody complexes after addition of rabbit antihuman fibrinogen antiserum to human serum. The increase in turbidity correlates very well with results obtained with the haemagglutination inhibition technique and has the advantage of a more objective quantitation. The reproducibility appears to be quite good. The turbidimetric method is independent of the colour of serum samples.

• References

• 1 Clauss A. 1957; Gerinnungsphysiologische Schnellmethode zur Bestimmung des Fibrinogens. Acta Haematologica 17: 237
• 2 Das PC, Allan AGE, Woodfield DG, Cash JD. 1967; Fibrin degradation products in sera of normal subjects. British Medical Journal 4: 718
• 3 Ferreira HC, Murat LG. 1963; An immunological method for demonstrating fibrin degradation products in serum and its use in the diagnosis of fibrinolytic states. British Journal of Haematology 9: 299
• 4 Gordon YB, Mc Neile AT, Martin MJ, Chard T. 1973; Specific and sensitive determination of fibrinogendegradation products by radioimmunoassay. Lancet II: 1168
• 5 Hjort P, Rapaport SI, Owren PA. 1955; A simple specific one-stage prothrombin assay. Journal of Laboratory and Clinical Medicine 46: 89
• 6 Marder V, Matchett MO, Sherry S. 1971; Detection of serum fibrinogen and fibrin degradation products. American Journal of Medicine 51: 71
• 7 Merskey C, Kleiner GJ, Johnson AJ. 1966; Quantitative estimation of split products of fibrinogen in human serum, relation to diagnosis and treatment. Blood 28: 1
• 8 Mertens BF, McDuffie FC, Bowie EJW, Orven CA. 1969; Rapid sensitive method for measuring fibrinogen split products in human serum. Mayo Clinic Proceedings 44: 144
• 9 Niléhn 1967; Separation and estimation of splitproducts of fibrinogen and fibrin in human serum. Thrombosis et Diathesis Haemorrhagica 18: 487
• 10 Vermijlen C, De Vreeker R, Verstraete M. 1963; A rapid enzymatic method for assay fibrinogen, Fibrin Polymerization Time (FPT-test). Clinica Chimica Acta 8: 418