The Erythrina Protease Inhibitor: Interactions with Tissue Plasminogen Activator

Christa Heussen-Schemmer; Edwin H Merrifield; Eugene B D Dowdle

doi:10.1055/s-0038-1646395

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1991; 66(02): 226-231
DOI: 10.1055/s-0038-1646395

Review Article

Schattauer GmbH Stuttgart

The Erythrina Protease Inhibitor: Interactions with Tissue Plasminogen Activator

Christa Heussen-Schemmer

The Department of Clinical Science and Immunology, University of Cape Town and the South African Medical Research Council, Human Cell Biology Research Unit, Observatory, South Africa

,

Edwin H Merrifield

The Department of Clinical Science and Immunology, University of Cape Town and the South African Medical Research Council, Human Cell Biology Research Unit, Observatory, South Africa

,

Eugene B D Dowdle

The Department of Clinical Science and Immunology, University of Cape Town and the South African Medical Research Council, Human Cell Biology Research Unit, Observatory, South Africa

› Institutsangaben

Abstract

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Summary

The Kunitz-type trypsin and tissue plasminogen activator (t-PA)-inhibitor from Erythrina caffra seeds was cleaved by trypsin at low pH to yield a disulphide linked two-chain molecule with reduced hydrophobicity. This change was used to separate cleaved from native inhibitor by phenyl-Sepharose chromatography. The inhibitor was not cleaved by t-PA. Trypsin, but not t-PA, catalysed resynthesis of the cleaved bond.

Although the cleaved protein retained inhibitory activity for both trypsin and t-PA, 6-10 times higher concentrations were required for equivalent inhibition.

Removal of the active site arginine (Arg₆₃) from the cleaved inhibitor by digestion with carboxypeptidase B resulted in a further loss of inhibitory activity towards both proteases. The activity of the inhibitor could also be decreased by modification of one susceptible arginine residue with peptidyl arginine deiminase. These results suggest that the trypsin-reactive site of the Erythrina inhibitor is also involved in the interaction between the inhibitor and t-PA.

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Referenzen

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