PAI-1 Plays an Important Role in the Expression of t-PA Activity in the Euglobulin Clot Lysis by Controlling the Concentration of Free t-PA

Tetsumei Urano; Kenichi Sumiyoshi; Michal H Pietraszek; Yumiko Takada; Akikazu Takada

doi:10.1055/s-0038-1646441

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1991; 66(04): 474-478
DOI: 10.1055/s-0038-1646441

Review Article

Schattauer GmbH Stuttgart

PAI-1 Plays an Important Role in the Expression of t-PA Activity in the Euglobulin Clot Lysis by Controlling the Concentration of Free t-PA

Authors

Tetsumei Urano

The Department of Physiology, Hamamatsu University, School of Medicine, Hamamatsu-shi, Japan
Kenichi Sumiyoshi

^*The Department of Surgery, Hamamatsu University, School of Medicine, Hamamatsu-shi, Japan
Michal H Pietraszek

The Department of Physiology, Hamamatsu University, School of Medicine, Hamamatsu-shi, Japan
Yumiko Takada

The Department of Physiology, Hamamatsu University, School of Medicine, Hamamatsu-shi, Japan
Akikazu Takada

The Department of Physiology, Hamamatsu University, School of Medicine, Hamamatsu-shi, Japan

Abstract

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Summary

The antigen levels of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) were assayed in the plasma and in the euglobulin fraction, and their contributions to the euglobulin clot lysis time (ECLT) and t-PA activity were analyzed. Total and free PAI-1 levels in both fractions showed significant positive correlation with ECLT (p <0.001), whereas t-PA antigen level did not have a high correlation coefficient with ECLT. t-PA activity showed significant negative correlation with ECLT (p <0.001) and positive correlation with free t-PA level (p <0.001), which was calculated by the ratio of the concentrations of t-PA-PAI-1 complex and the free PAI-1. Thus free t-PA seems to dissolve the euglobulin clot and its concentration seems to be controlled by the concentration of free PAI-1. These findings were confirmed by the analyses of the effects of C1-inactivator and antibody against t-PA to regular ECLT and kaolin activated ECLT, the latter of which was only inhibited by the addition of C1-inactivator whereas the former was inhibited by anti-t-PA antibody.

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References

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