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DOI: 10.1055/s-0038-1646539
Thrombin Stimulates Inositol Phosphate Accumulation and Prostacyclin Synthesis in Human Endothelial Cells from Umbilical Vein but Not from Omentum
Publikationsverlauf
Received 17. Juni 1988
Accepted after revision 28. September 1988
Publikationsdatum:
24. Juli 2018 (online)
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Summary
We have compared the effects of thrombin on the accumulation of inositol phosphates and the synthesis of prostacyclin in cultured human endothelial cells from umbilical vein and the microvasculature of omentum. Active human thrombin induced a dose-dependent accumulation of inositol phosphates and a concomitant synthesis of prostacyclin in endothelial cells from human umbilical vein. However, thrombin at all concentrations tested was unable to stimulate inositol phosphate accumulation and prostacyclin synthesis in microvascular endothelial cells from human omentum. Bradykinin was able to stimulate these effects in both types of cell. These results demonstrate that although inositol phosphate turnover is an initial event associated with prostacyclin synthesis in endothelial cells, there are differences in the way microvascular endothelial cells respond to thrombin.
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References
- 1 Gimbrone MA. Vascular endothelium: nature’s blood container. In: Vascular Endothelium in Hemostasis and Thrombosis. Gimbrone MJ. (ed) Churchill Livingstone; Edinburgh: 1986. pp 1-13
- 2 Weksler B, Ley CW, Jaffe EA. Stimulation of endothelial cell prostacyclin production by thrombin, trypsin, and the ionophore A 23187. J Clin Invest 1978; 62: 923-930
- 3 Czervionke RL, Smith JB, Hoak JC, Fry GL, Haycraft DL. Use of a radioimmunoassay to study thrombin-induced release of PGI2 from cultured endothelium. Thromb Res 1979; 14: 781-786
- 4 Hong SL. Effect of bradykinin and thrombin on prostacyclin synthesis in endothelial cells from calf and pig aorta and human umbilical cord vein. Thromb Res 1986; 18: 787-795
- 5 Moncada S, Grygelewski R, Bunting S, Vane JR. An enzyme isolated from arteries transforms prostaglandin endoperoxides to an unstable substance that inhibits platelet aggregation. Nature 1976; 263: 663-665
- 6 Johnson RA, Morton DR, Kinner JH, Gorman RR, McGuire JC, Sun FF. The chemical structure of prostaglandin X (prostacyclin). Prostaglandins 1976; 12: 915-928
- 7 Berridge MJ, Irvine RF. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature 1984; 312: 315-321
- 8 Nishizuka Y. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature 1984; 308: 693-698
- 9 Derian CK, Moskowitz MA. Polyphosphoinositide hydrolysis in endothelial cells and carotid artery segments. J Biol Chem 1986; 261: 3831-3837
- 10 Lambert TL, Kent RS, Whorton AR. Bradykinin stimulation of inositol polyphosphate production in porcine aortic endothelial cells. J Biol Chem 1986; 261: 15288-15293
- 11 Resink TJ, Grigorian GY, Moldabaeva AK, Danilov SM, Buehler FR. Histamine-induced phosphoinositide metabolism in cultured human umbilical vein endothelial cells. Association with thromboxane and prostacyclin release Biochem Biophys Res Commun 1987; 144: 438-446
- 12 Jaffe EA, Grulich J, Weksler BB, Hempel G, Watanabe K. Correlation between thrombin-induced prostacyclin production and inositol trisphosphate and cytosolic free calcium levels in cultured human endothelial cells. J Biol Chem 1987; 262: 8557-8565
- 13 Zetter BR. The endothelial cells of large and small vessels. Diabetes 1981; 30 Suppl (Suppl. 02) 24-38
- 14 Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphological and immunological criteria J Clin Invest 1973; 52: 2745-2756
- 15 Kern PA, Knedler A, Eckel RH. Isolation and culture of micro vascular endothelium from adipose tissue. J Clin Invest 1983; 71: 1822-1829
- 16 Alles JU, Bosslet K. Immunohistochemical and immunochemical characterization of a new endothelial cell-spezific antigen. J His- tochem Cytochem 1986; 34: 209-214
- 17 Voyta JC, Via DP, Butterfield CE, Zetter BR. Identification and isolation of endothelial cells based on their increased uptake of acetylated low-density lipoprotein. J Cell Biol 1984; 99: 2034-2040
- 18 Richards DE, Irvine RF, Dawson RM C. Hydrolysis of membrane phospholipases of rat liver. Biochem J 1979; 182: 599-606
- 19 Downes CP, Michell RH. The polyphosphoinositide phosphodiesterase of erythrocyte membranes. Biochem J 1981; 198: 133-140
- 20 Berridge MJ, Dawson RM C, Downes CP, Heslop JP, Irvine RF. Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides. Biochem J 1983; 212: 473-482
- 21 Tollefson DM, Feagler JR, Majerus PW. The binding of thrombin to the surface of human platelets. J Biol Chem 1974; 249: 2646-2651
- 22 Lapetina EG. Incorporation of synthetic 1, 2-diacylglycerol into platelet phosphatidylinositol is increased by cyclic AMP. FEBS Lett 1986; 195: 111-114
- 23 Petty RG, Pearson JD, Morgan DM L, Mahler RF. Stimulation of endothelial cell growth by sera from diabetic patients with retinopathy. Lancet 1988; 1: 208-211
- 24 Pearson JD, Carleton JS, Hutchings A. Prostacyclin releases stimulated by thrombin or bradykinin in porcine endothelial cells cultured from aorta and umbilical vein. Thromb Res 1983; 29: 115-124
- 25 Goldsmith JC, Kiskar CT. Thrombin-endothelial cell interactions: critical importance of endothelial cell vessel origin. Thromb Res 1983; 25: 131-136
- 26 Speiser W, Anders E, Preissner KT, Wagner O, Müller-Berghaus G. Differences in coagulant and fibrinolytic activities of cultured human endothelial cells derived from omental tissue microvessels and umbilical vein. Blood 1987; 69: 964-967