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Thromb Haemost 1989; 61(02): 217-224
DOI: 10.1055/s-0038-1646562
Original Article
Schattauer GmbH Stuttgart

Endothelial Cells and Normal Circulating Haemopoietic Cells Share a Number of Surface Antigens

Emmanuel J Favaloro
The Department of Haematology Westmead Hospital, NSW, Australia
,
Nick Moraitis
The Department of Haematology Westmead Hospital, NSW, Australia
,
Jerry Koutts
The Department of Haematology Westmead Hospital, NSW, Australia
,
Thomas Exner
The Department of Haematology Westmead Hospital, NSW, Australia
,
Kenneth F Bradstock
The Department of Haematology Westmead Hospital, NSW, Australia
› Author Affiliations
Further Information

Publication History

Received 12 October 1988

Accepted after revision 30 December 1988

Publication Date:
30 June 2018 (online)

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Summary

Human endothelial cells, cultured from umbilical cord veins, have been evaluated for expression of a large number of cell surface antigens with known haemopoietic, particularly myeloid, cell distribution. This was achieved by evaluating endothelial reactivity (using non-fixed cells) with groups of monoclonal antibodies (MAB) belonging to distinct Clusters of Differentiation (CD), as defined by the Third International Workshop on Leukocyte Differentiation Antigens (ILWS). Results indicate that many antigens known to be present on haemopoietic cells, including those on platelets, are co-expressed on endothelial cells. The most intense degree of reactivity was seen using MAB to CD-9 and CD-13, although significant reactivity was also observed using MAB to CD-31 and CD-32. Data also suggests weak binding to endothelial cells of MAB belonging to CD-14, CD-15 and CD-16. A number of unclustered MAB reactive with haemopoietic antigens can also be shown to bind to endothelial cells.

Keywords

Endothelial cells - Cell surface antigens - Haemopoietic cell antigens - Monoclonal antibodies
 

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